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Journals / Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2026 / Cilt: 19 Sayı: 2

Investigation of In Vitro Propagation Potentials of Prunus Rootstocks Developed by Selection and Hybridization

Seleksiyon ve Melezleme Yoluyla Geliştirilen Prunus Anaçlarının In Vitro Çoğaltım Potansiyellerinin Araştırılması

Remzi Uğur(Gaziantep Üniversitesi Nurdağı Meslek Yüksekokulu)0000-0001-6717-1689
Esra Bulunuz Palaz(East Mediterranean Transitional Agricultural Research Institute)0000-0001-7795-496X
Özkan Altun(East Mediterranean Transitional Agricultural Research Institute)0000-0002-5092-2519
Sevgi Paydaş(CUKUROVA UNIVERSITY)0000-0001-5781-8581
Pages
469–482
Publication Date
2026-08-31
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The findings are expected to contribute significantly to stone fruit rootstock breeding programs.

📌 3 Key Takeaways

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Objective & Hypothesis:This study investigated in vitro regeneration and rooting performance in 41 rootstock candidates obtained through selection and hybridization breeding methods.
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Key Findings & Data:The highest shoot formation (13.66 shoots/explant) was observed in the control rootstock Myrobolan 29C, followed by GN-22 (12.66), KL-30 (12.66), and KL-6 (12.00).
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Conclusion & Impact:The findings are expected to contribute significantly to stone fruit rootstock breeding programs.
🔬Design:Modeling & Simulation
📍Scope:2025 Verileri
⚙️Variables:In vitro · rejenerasyon · Prunus

Abstract

This study investigated in vitro regeneration and rooting performance in 41 rootstock candidates obtained through selection and hybridization breeding methods. The highest shoot formation (13.66 shoots/explant) was observed in the control rootstock Myrobolan 29C, followed by GN-22 (12.66), KL-30 (12.66), and KL-6 (12.00). Among the 46 genotypes, 26 (56.5%) achieved a success rate of ≥3.3 shoots/explant. Shoot lengths varied between 2.29–30.36 mm, with 71.7% of shoots reaching ≥14.6 mm. Callus formation (33.3–55.0%) and vitrification (11.7–33.3%) were observed in only three genotypes. Regeneration failure was detected in six rootstock candidates (13%). KL-59 exhibited the highest rooting performance (7.66 roots/explant), while KL-2 showed the lowest (1.33 roots). Among the genotypes, 87.5% achieved ≥2.33 roots/explant. Root lengths ranged from 4.9–25.75 mm, with 92.5% exceeding 10 mm. The results were consistent with previously reported genotype-dependent variations in Prunus species (Koaym, 2025). Notably, the rootstock coded KL-59 demonstrated superior performance. The low vitrification rates observed at the end of the study reflected the success of protocol optimization. Furthermore, this study revealed that in vitro micropropagation success in Prunus rootstocks varies significantly depending on genotype and highlighted the importance of identifying high-performance clones like KL-59 for commercial production. The findings are expected to contribute significantly to stone fruit rootstock breeding programs.

Özet

Bu çalışmada, seleksiyon ve melezleme ıslah yöntemleriyle elde edilen 41 anaç adayının in vitro rejenerasyon ve köklenme performansları araştırılmıştır. En yüksek sürgün oluşumu (13,66 sürgün/eksplant) kontrol anaç olan Myrobolan 29C'de gözlemlenmiş, bunu sırasıyla GN-22 (12,66), KL-30 (12,66) ve KL-6 (12,00) takip etmiştir. Toplam 46 genotipin 26'sında (%56,5) ≥3,3 sürgün/eksplant başarı oranına ulaşılmıştır. Sürgün uzunlukları 2,29–30,36 mm aralığında değişmiş ve sürgünlerin %71,7'si ≥14,6 mm uzunluğa erişmiştir. Yalnızca üç genotipte kallus oluşumu (%33,3–55,0) ve vitrifikasyon (%11,7–33,3) gözlenmiştir. Altı anaç adayında (%13) rejenerasyon başarısızlığı tespit edilmiştir. En yüksek köklenme performansı KL-59 genotipinde (7,66 kök/eksplant) kaydedilirken, en düşük performans KL-2'de (1,33 kök) gözlemlenmiştir. Genotiplerin %87,5'i ≥2,33 kök/eksplant oranına ulaşmıştır. Kök uzunlukları 4,9–25,75 mm aralığında olup, köklerin %92,5'i 10 mm'yi aşmıştır. Elde edilen sonuçlar, Prunus türlerinde daha önce rapor edilen genotip-bağımlı varyasyonlarla uyumludur (Koaym, 2025). Özellikle KL-59 koduyla belirtilen anaç, üstün performans sergilemiştir. Çalışma sonunda gözlemlenen düşük vitrifikasyon oranları, protokol optimizasyonunun başarısını yansıtmaktadır. Ayrıca bu çalışma, Prunus anaçlarında in vitro mikroçoğaltım başarısının genotipe bağlı olarak önemli ölçüde değiştiğini ortaya koymuş ve KL-59 gibi yüksek performanslı klonların ticari üretim için belirlenmesinin önemini vurgulamıştır. Bulguların, sert çekirdekli meyve anaç ıslah programlarına önemli katkı sağlaması beklenmektedir.

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Cite This Article

Uğur, R., Palaz, E. B., Altun, Ö., Paydaş, S., *, R. U. (2026). Investigation of In Vitro Propagation Potentials of Prunus Rootstocks Developed by Selection and Hybridization. Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 19 Sayı: 2, 469–482. https://doi.org/10.18185/erzifbed.1694924

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