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Journals / Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2026 / Cilt: 19 Sayı: 2

Investigation of Anticancer Potential of Prophet's Broom (Artemisia annua) in Tokat Populations

Elif Aktürk Bozdemir(TOKAT GAZİOSMANPAŞA ÜNİVERSİTESİ, TOKAT MESLEK YÜKSEKOKULU)0009-0008-4226-7834
Pages
288–308
Publication Date
2026-08-31
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

The results showed that ethanol extracts had the highest artemisinin content (0.76-0.98%) and the most potent anticancer activity.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, the anticancer effects of the extracts prepared with various solvents (ethanol, methanol, n-hexane, and water) of different parts (leaf, flower, and root) of Cornflower Broom (Artemisia annua L.) collected from the Tokat region were investigated.
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Key Findings & Data:The cytotoxic effects of the extracts on human prostate cancer (DU145, PC-3), colon cancer (HCT116), and lung cancer (A549) cell lines were assessed through MTT and CCK-8 assays.
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Conclusion & Impact:The results showed that ethanol extracts had the highest artemisinin content (0.76-0.98%) and the most potent anticancer activity.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Artemisia annua · Peygamber Süpürgesi · artemisinin

Abstract

In this study, the anticancer effects of the extracts prepared with various solvents (ethanol, methanol, n-hexane, and water) of different parts (leaf, flower, and root) of Cornflower Broom (Artemisia annua L.) collected from the Tokat region were investigated. The extracts' total phenolic and flavonoid contents were quantified using spectrophotometric techniques, while artemisinin and other bioactive compounds were analyzed via HPLC. The cytotoxic effects of the extracts on human prostate cancer (DU145, PC-3), colon cancer (HCT116), and lung cancer (A549) cell lines were assessed through MTT and CCK-8 assays. Apoptotic activity, cell cycle progression, ROS production, and DNA damage were also investigated. The results showed that ethanol extracts had the highest artemisinin content (0.76-0.98%) and the most potent anticancer activity. The extracts prepared from leaf samples showed a significant cytotoxic effect, especially in PC-3 and HCT116 cell lines (IC₅₀: 28.4-42.7 µg/mL), and increased apoptosis by 54-67% compared to the control. Our study showed that A. annua, which grows in the Tokat region, has significant anticancer potential thanks to its bioactive components, especially artemisinin. This study shows the possible use of A. annua in cancer treatment and sheds light on evaluating Turkey's natural resources that can contribute to Turkey's bioeconomy.

Özet

Bu çalışmada, Tokat yöresinden toplanan Peygamber Süpürgesi (Artemisia annua L.) bitkisinin farklı kısımlarının (yaprak, çiçek ve kök) çeşitli çözücülerle (etanol, metanol, n-hekzan ve su) hazırlanan ekstraktlarının antikanser etkileri incelenmiştir. Ekstraktların toplam fenolik ve flavonoid içerikleri spektrofotometrik yöntemlerle belirlenmiş, HPLC ile artemisinin ve diğer biyoaktif bileşenler tespit edilmiştir. İnsan prostat kanseri (DU145, PC-3), kolon kanseri (HCT116) ve akciğer kanseri (A549) hücre hatlarında sitotoksik etkileri MTT ve CCK-8 yöntemleri kullanılarak değerlendirilmiştir. Apoptotik aktivite, hücre döngüsü, ROS oluşumu ve DNA hasarı analizleri yapılmıştır. Araştırma sonuçları, etanol ekstraktlarının en yüksek artemisinin içeriğine (%0,76-0,98) ve en güçlü antikanser aktiviteye sahip olduğunu göstermiştir. Yaprak örneklerinden hazırlanan ekstraktlar, özellikle PC-3 ve HCT116 hücre hatlarında belirgin sitotoksik etki göstermiş (IC₅₀: 28,4-42,7 µg/mL), kontrole kıyasla apoptozu %54-67 oranında artırmıştır. Çalışmamız, Tokat yöresinde yetişen A. annua'nın, artemisinin başta olmak üzere içerdiği biyoaktif bileşenler sayesinde önemli antikanser potansiyeline sahip olduğunu göstermiştir. Bu çalışma, A. annua'nın kanser tedavisinde kullanım potansiyelini göstermekte ve Türkiye'nin biyoekonomisine katkıda bulunabilecek doğal kaynaklarının değerlendirilmesine ışık tutmaktadır.

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Cite This Article

Bozdemir, E. A. (2026). Investigation of Anticancer Potential of Prophet's Broom (Artemisia annua) in Tokat Populations. Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 19 Sayı: 2, 288–308. https://doi.org/10.18185/erzifbed.1771820

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