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Journals / Journal of Istanbul Veterinary Sciences / 2026 / Cilt: 10 Sayı: 2

The achievements of in vitro maturation after warming to the cryopreserved bovine oocytes by different vitrification methods

Farklı vitrifikasyon yöntemleri ile dondurulan sığır oositlerinin çözdürme sonrası in vitro maturasyon başarıları

Şöhret Güler(Burdur Mehmet Akif Ersoy Üniversitesi)0000-0003-1334-290X
Afşin Köker(Burdur Mehmet Akif Ersoy)0000-0001-6580-1309
Pages
138–147
Publication Date
2026-08-31
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, both OPS and SSV methods are appropriate for vitrification of immature bovine oocytes.

📌 3 Key Takeaways

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Objective & Hypothesis:The susceptibility of bovine oocytes to freezing damage highlights the need to develop a vitrification methodology that will reduce cryosensitivity.
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Key Findings & Data:Immature oocytes were aspirated from the follicles on the ovaries of slaughtered bovines from a local abattoir were investigated under a stereomicroscope and 450 immature oocytes that had at least three layers of compacted cumulus cells were selected for this study.
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Conclusion & Impact:In conclusion, both OPS and SSV methods are appropriate for vitrification of immature bovine oocytes.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Açık Uçlu Modifiye Payet · İmmatür oosit · Katı Yüzey Vitrifikasyonu

Abstract

The susceptibility of bovine oocytes to freezing damage highlights the need to develop a vitrification methodology that will reduce cryosensitivity. This study was conducted to use two different vitrification methods to cryopreservation of immature oocytes and then examine how they affect the maturation success. The methods were used are Open Pulled Straw (OPS) and Solid Surface Vitrification (SSV). Immature oocytes were aspirated from the follicles on the ovaries of slaughtered bovines from a local abattoir were investigated under a stereomicroscope and 450 immature oocytes that had at least three layers of compacted cumulus cells were selected for this study. Three different groups (OPS, SSV and Control), that each consists of 150 oocytes were formed. Oocytes in the OPS and SSV groups were incubated for maturation after vitrification-warming, while oocytes in the control group were incubated for maturation non-vitrified. After maturation, oocytes were examined for the cumulus expansion and presence of polar body. When the polar body in oocytes stripped from the cumulus cells were examined, the polar body 23.19% (32/138) in the OPS group, 20.90% (28/134) in the SSV group, and 78.83% (108/137) oocyte in the control group were determined. The difference between OPS and SSV groups was not statistically significant (p>0.05). However, the results of control group was significantly higher than the vitrification groups (p<0.001). In conclusion, both OPS and SSV methods are appropriate for vitrification of immature bovine oocytes.

Özet

Sığır oositlerinin donma hasarına duyarlılığı, kriyosensitiviteyi azaltacak bir vitrifikasyon metodolojisi geliştirme ihtiyacını vurgulamaktadır. Bu çalışma, immatür oositlerin dondurulması için iki farklı vitrifikasyon yönteminin kullanılması ve bunların maturasyon başarısını nasıl etkilediğinin incelenmesi amacıyla yapıldı. Kullanılan yöntemler Açık Uçlu Modifiye Payet (OPS) ve Katı Yüzey Vitrifikasyonu (SSV)'dur. Yerel bir mezbahada kesilen sığırların ovaryumlarındaki foliküllerden aspire edilen immatür oositler, stereomikroskop altında incelendi. Bu çalışma için en az üç kat sıkı kumulus hücresine sahip 450 immatür oosit seçildi. Her biri 150 oositten oluşan üç farklı grup (OPS, SSV ve Kontrol) oluşturuldu. OPS ve SSV gruplarındaki oositler, vitrifikasyon-çözdürme işleminden sonra maturasyon için inkübe edilirken, kontrol grubundaki oositler vitrifikasyon yapılmadan maturasyon için inkübe edildi. Maturasyondan sonra, oositler kumulus ekspansiyonu ve polar body varlığı açısından incelendi. Kumulus hücrelerinden soyulan oositlerde polar body incelendiğinde, OPS grubunda %23,19 (32/138), SSV grubunda %20,90 (28/134) ve kontrol grubunda %78,83 (108/137) oranında polar body tespit edildi. OPS ve SSV grupları arasındaki fark istatistiksel olarak anlamlı değildi (p>0,05). Bununla birlikte, kontrol grubunun sonuçları vitrifikasyon gruplarından anlamlı derecede daha yüksekti (p<0,001). Sonuç olarak hem OPS hem de SSV yöntemleri immatür sığır oositlerinin vitrifikasyonu için uygundur.

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Cite This Article

Güler, Ş., Köker, A. (2026). The achievements of in vitro maturation after warming to the cryopreserved bovine oocytes by different vitrification methods. Journal of Istanbul Veterinary Sciences, Cilt: 10 Sayı: 2, 138–147. https://doi.org/10.30704/http-www-jivs-net.1893194

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