Journals / Mediterranean Agricultural Sciences / 2006 / Cilt: 19 - Sayı: 2

A Direct RP-HPLC Determination of Phenolic Compounds in Turkish Red Wines

Türk Kırmızı Şaraplarında Fenolik Bileşiklerin Direkt RP-HPLC ile Belirlenmeleri

Pages
229–234
Publication Date
2006-06-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result, it was determined that types and concentrations of phenolics changed according to the wines from different cultivars.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Red wines from four different Turkish grape cultivars were analyzed in order to determine their phenolic contents.
📊
Key Findings & Data:For the analyses, reversed phase-high performance liquid chromatography (RP-HPLC) coupled with diode array detection was used.
💡
Conclusion & Impact:As a result, it was determined that types and concentrations of phenolics changed according to the wines from different cultivars.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Türk Şarapları · Fenolik Bileşikler · RP-HPLC

Abstract

Red wines from four different Turkish grape cultivars were analyzed in order to determine their phenolic contents. For the analyses, reversed phase-high performance liquid chromatography (RP-HPLC) coupled with diode array detection was used. The concentrations of eight phenolic acid standards (ferulic, o-coumaric, p-coumaric, caffeic, syringic, trans-cinnamic, chlorogenic and gallic acids) and five flavonoid standards ((+)-catechin, (-)-epicatechin, quercetin, vanillin and rutin) were used to determine characteristic differences among red wines from Kalecik karası, Öküzgözü, Boğazkere and Papazkarası cultivars grown in the viticultural region of Türkiye. In the wine samples, the most abundant phenolics (+)-catechin (17.82–33.59 mg L-1) as flavonoid and gallic acid (13.25-16.39 mg L-1) as phenolic acid while chlogenic acid was not detected in any samples. As a result, it was determined that types and concentrations of phenolics changed according to the wines from different cultivars.

Özet

Dört farklı Türk üzüm çeşidinden yapılan kırmızı şaraplar, fenolik içeriklerini belirlemek amacıyla analiz edilmişlerdir. Analiz için diode array detectör eşliğinde ters fazlı-yüksek basınçlı sıvı kromotografisi (RP-HPLC) kullanılmıştır. Türkiye’de yetiştirilen Kalecik karası, Öküzgözü, Boğazkere ve Papazkarası üzüm çeşitlerinden elde edilen kırmızı şaraplar arasındaki karakteristik farkları belirlemek için, sekiz fenolik asit (ferulik, o-kumarik, pkumarik, kaffeik, siyringik, trans-sinnamik, klorogenik ve gallik asitler) ile beş flavonoid ((+)-kateşin, (-)-epikateşin, kuersetin, vanillin ve rutin) standartı kullanılmıştır. Şarap örneklerinde en fazla bulunan fenolikler, flavonoidlerden (+)-kateşin (17,82–33,59 mg L-1) ve fenolik asitlerden de gallik asit (13,25-16,39 mg L-1) olurken, klorogenik asit hiçbir şarap örneğinde belirlenememiştir. Sonuç olarak, fenolik maddelerin tip ve konsantarsyonlarının farklı çeşitlerden elde edilen şaraplara göre değiştiği belirlenmiştir

🔖

Cite This Article

ÖZKAN, G., BAYDAR, N. G. (2006). A Direct RP-HPLC Determination of Phenolic Compounds in Turkish Red Wines. Mediterranean Agricultural Sciences, Cilt: 19 - Sayı: 2, 229–234. https://ajindex.com/articles/123748

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (11)

  1. Abril, M., Negueruela, A.I., Pérez, C., Juan, T. and Estopanan, G., 2005. Preliminary Study of Resveratrol Content in Aragón Red and Rosé Wines. Food Chemistry, 92(4): 729-736. Andrade,P. B., Mendes, Falco, G.V., Valentão, P. and Seabra, R. M., 2001. Preliminary Study of Flavonols in Port Wine Grape Varieties Food Chemistry,: 397-399.
  2. Bayhan, A., 2004. Determination of some phenolic compounds by using GC-MS methods in wines produced from different kalecik karası clones. Ankara University, p 37, Master Thesis. Briviba, K. and Sies, H., 1994. Nonenzymathic Antioxidant Defence Systems. In: B. Frei (Editor), Natural Antioxidant in Human Health and Disease. Academic Press Inc., New York, USA, pp. 107-128.
  3. Caponio, F., Alloggio, V. and Gomes, T., 1999. Phenolic Compounds of Virgin Olive Oil:Influence of Paste Preparation Techniques. Food Chemistry, 64: 203-209.
  4. Castellari M., Sartini E., Fabiani A., Arfelli G. and Amati A., 2002. Analysis of Wine Phenolics by High Performance Liquid Chromatography Using a Monolithic Type Column. Journal of Chromatography A, 973 (1-2): 221-227.
  5. De Villiers A., Vanhoenacker G., Majek P. and Sandra P., 2004. Determination of Anthocyanins in Wine by Direct Injection Liquid Chromatography-Diode Array Detection-Mass Spectrometry and Classification of Wines Using Discriminant Analysis. Journal of Chromatography A, 1054 (1-2): 195-204.
  6. Gambelli, L. and Santaroni, G. P., 2004. Polyphenols Content in Some Italian Red Wines of Different Geographical Origins. Journal of Food Composition and Analysis, 17(5): 613-618.
  7. Goldberg, D.M., Karumanchiri, A., Tsang, E., and Soleas, G.J., 1998. Catechin and epicatechin concentrations of red wines: Regional and cultivar-related differences. American Journal of Enology and Viticulture, 49: 23-34.
  8. Huang, M., Ho, C. and Lee, C.,1992. Phenolic Compounds in Foods and Their Effects on Health. American Chemical Society, Washington, USA. Husain, S.R., Cillart, J. and Cillart, P., 1987. Hydroxy Radical Scavenging Activity of Flavonoids. Phytochemistry, 26 (9): 2489-2491.
  9. Ibern-Gomez M., Andres-Lacueva C., Lamuela- Raventos R.M. and Waterhouse A.L., 2002. Rapid HPLC Analysis of Phenolic Compounds in Red Wines. American Journal of Enology And Viticulture, 53 (3): 218-221.
  10. Kallithraka, S., Arvanitoyannis, I., El-Zajouli, A. and Kefalas, P., 2001. The Application of an Improved Method for Trans-Resveratrol to Determine the Origin of Greek Red Wines. Food Chremistry, 75: 355-363.
  11. Kılınç, E. and Kalkan, H., 2003. Hi