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Journals / Turkish Journal of Veterinary and Animal Sciences / 2007 / Cilt: 31 - Sayı: 5

Expression of the b-(1,3-1,4)-Glucanase Gene in Streptococcus salivarius subsp. thermophilus

Meltem AŞAN
Numan ÖZCAN
Pages
319–324
Publication Date
2007-05-01
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

The enzyme expressed by all the recombinant bacteria resisted denaturation and somehow remained soluble after heat treatment from 37 to 100 ºC for 15 min.

📌 3 Key Takeaways

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Objective & Hypothesis:The purpose of this study was the transformation and expression of the b -(1,3-1,4)-glucanase (lichenase) gene in Streptococcus salivarius subsp. thermophilus to create a recombinant probiotic for poultry and improve the thermostability of the lichenase enzyme.
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Key Findings & Data:The recombinant plasmid TL1R containing the b -(1,3-1,4)-glucanase gene was introduced into S. salivarius subsp. thermophilus by electrotransformation.
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Conclusion & Impact:The enzyme expressed by all the recombinant bacteria resisted denaturation and somehow remained soluble after heat treatment from 37 to 100 ºC for 15 min.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Streptococcus salivarius subsp. thermophilus · b -(1 · 3-1

Abstract

The purpose of this study was the transformation and expression of the b -(1,3-1,4)-glucanase (lichenase) gene in Streptococcus salivarius subsp. thermophilus to create a recombinant probiotic for poultry and improve the thermostability of the lichenase enzyme. The recombinant plasmid TL1R containing the b -(1,3-1,4)-glucanase gene was introduced into S. salivarius subsp. thermophilus by electrotransformation. The expressing of the b -(1,3-1,4)-glucanase gene in S. salivarius subsp. thermophilus was confirmed on lichenan plate, SDS-PAGE, and zymogram analysis. The b -(1,3-1,4)-glucanase enzyme expressed by S. salivarius subsp. thermophilus cells seemed to increase its capacity for thermoresistance and so it maintained its activity at 70 ºC for 15 min. In contrast, the enzyme produced by Lactococcus lactis and Escherichia coli cells easily ceased activity when exposed to the same temperature. The enzyme expressed by all the recombinant bacteria resisted denaturation and somehow remained soluble after heat treatment from 37 to 100 ºC for 15 min.

Özet

The purpose of this study was the transformation and expression of the b -(1,3-1,4)-glucanase (lichenase) gene in Streptococcus salivarius subsp. thermophilus to create a recombinant probiotic for poultry and improve the thermostability of the lichenase enzyme. The recombinant plasmid TL1R containing the b -(1,3-1,4)-glucanase gene was introduced into S. salivarius subsp. thermophilus by electrotransformation. The expressing of the b -(1,3-1,4)-glucanase gene in S. salivarius subsp. thermophilus was confirmed on lichenan plate, SDS-PAGE, and zymogram analysis. The b -(1,3-1,4)-glucanase enzyme expressed by S. salivarius subsp. thermophilus cells seemed to increase its capacity for thermoresistance and so it maintained its activity at 70 ºC for 15 min. In contrast, the enzyme produced by Lactococcus lactis and Escherichia coli cells easily ceased activity when exposed to the same temperature. The enzyme expressed by all the recombinant bacteria resisted denaturation and somehow remained soluble after heat treatment from 37 to 100 ºC for 15 min.

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Cite This Article

AŞAN, M., ÖZCAN, N. (2007). Expression of the b-(1,3-1,4)-Glucanase Gene in Streptococcus salivarius subsp. thermophilus. Turkish Journal of Veterinary and Animal Sciences, Cilt: 31 - Sayı: 5, 319–324. https://ajindex.com/articles/123680

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