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Journals / Turkish Journal of Veterinary and Animal Sciences / 2017 / Cilt: 41 - Sayı: 6

Development of multiplex RT-PCR for detection and differentiation of foot-and-mouth disease virus O and A serotypes in Turkey

Beyhan SAREYYÜPOĞLU
İbrahim BURGU
Pages
764–769
Publication Date
2017-12-20
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The study showed that mRT-PCR was more efficient than ELISA and concluded that the technique can be used as an alternative to ELISA for molecular typing of FMDV samples.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, a two-step multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was developed and evaluated using designed primers for the simultaneous detection and differentiation of Turkish foot-and-mouth disease virus (FMDV) serotypes A and O from clinical samples.
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Key Findings & Data:In the method, a mix of one universal reverse primer designed from the 2B gene and two serotype-specific forward primers designed from the hypervariable regions of the capsid VP1-1D coding gene of FMDV were used.
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Conclusion & Impact:The study showed that mRT-PCR was more efficient than ELISA and concluded that the technique can be used as an alternative to ELISA for molecular typing of FMDV samples.
🔬Design:Research Article
📍Scope:2006 · 272 samples
⚙️Variables:Foot-and-mouth disease virus · diagnosis · mRT-PC

Abstract

In this study, a two-step multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was developed and evaluated using designed primers for the simultaneous detection and differentiation of Turkish foot-and-mouth disease virus (FMDV) serotypes A and O from clinical samples. In the method, a mix of one universal reverse primer designed from the 2B gene and two serotype-specific forward primers designed from the hypervariable regions of the capsid VP1-1D coding gene of FMDV were used. Totally, 272 FMDV-infected samples collected between 2006 and 2008 constituted the material of the study. mRT-PCR was compared to ELISA in terms of positivity percentage (%) and the difference between the two tests was found to be statistically significant (P < 0.05). In the study, 148 (54.4%) of 272 samples were undetermined by ELISA. Ninety-nine (66.8%) of these 148 undetermined samples were found FMDV RNA-positive with mRT-PCR. Consequently, the diagnostic sensitivity and specificity of mRT-PCR was determined as 95% and 84%, respectively. The study showed that mRT-PCR was more efficient than ELISA and concluded that the technique can be used as an alternative to ELISA for molecular typing of FMDV samples. This is the first report providing the differentiation of FMDV serotypes A and O in Turkey by mRT-PCR.

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Cite This Article

SAREYYÜPOĞLU, B., BURGU, İ. (2017). Development of multiplex RT-PCR for detection and differentiation of foot-and-mouth disease virus O and A serotypes in Turkey. Turkish Journal of Veterinary and Animal Sciences, Cilt: 41 - Sayı: 6, 764–769. https://ajindex.com/articles/122472

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