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Journals / Turkish Journal of Veterinary and Animal Sciences / 2017 / Cilt: 41 - Sayı: 6

Identification of Candida strains with nested PCR in bovine mastitis and determination of antifungal susceptibilities

Göksel ERBAŞ
Uğur PARIN
Şükrü KIRKAN
Serap SAVAŞAN
Mehmet Volkan ÖZAVCI
Hafize Tuğba YÜKSEL
Pages
757–763
Publication Date
2017-12-20
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to antifungal test results, the isolates were found to be susceptible to ketoconazole (78.3%) and resistant to flucytosine (91.3%), amphotericin (82.6%), miconazole and nystatin (73.9%), and fluconazole (69.5%).

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Mastitis is one of the biggest problems of dairy cattle industries.
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Key Findings & Data:A total of 260 mastitic milk samples were collected from different farms.
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Conclusion & Impact:According to antifungal test results, the isolates were found to be susceptible to ketoconazole (78.3%) and resistant to flucytosine (91.3%), amphotericin (82.6%), miconazole and nystatin (73.9%), and fluconazole (69.5%).
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Mastitis · nested PCR · antifungal

Abstract

Mastitis is one of the biggest problems of dairy cattle industries. In recent years, fungal agents have been frequently reported among the causative agents for mastitis. Among the fungal agents, Candida species are the most common. This study's aims were the isolation, identification, and determination of antifungal susceptibility of Candida species causing mycotic mastitis in cattle. A total of 260 mastitic milk samples were collected from different farms. Identification was conducted by rapid diagnostic tests and nested PCR method; 6 different antifungal agents were examined. Candida sp. was detected in 46 (17.7%) of the 260 mastitic milk samples. Based on API 20 C AUX and nested PCR test results, 6 different species of Candida were identified. C. tropicalis was the predominant one (26.1%), followed by C. parasilosis (21.7%), C. kefyr and C. krusei (17.4% each), C. rugosa (13%), and C. glabrata (4.4%). A total of 46 strains were confirmed by PCR. According to antifungal test results, the isolates were found to be susceptible to ketoconazole (78.3%) and resistant to flucytosine (91.3%), amphotericin (82.6%), miconazole and nystatin (73.9%), and fluconazole (69.5%).

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Cite This Article

ERBAŞ, G., PARIN, U., KIRKAN, Ş., SAVAŞAN, S., ÖZAVCI, M. V., YÜKSEL, H. T. (2017). Identification of Candida strains with nested PCR in bovine mastitis and determination of antifungal susceptibilities. Turkish Journal of Veterinary and Animal Sciences, Cilt: 41 - Sayı: 6, 757–763. https://ajindex.com/articles/122460

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References (11)

  1. Krukowski H, Lisowski A, Rózański P, Skórka A. Yeasts and algae isolated from cows with mastitis in the southeastern part of Poland. Pol J Vet Sci 2006; 9: 181-184.
  2. Dworecka-Kaszak B, Krutkiewicz A, Szopa D, Kleczkowski M, Bieganska M. High prevalence of Candida yeast in milk samples from cows suffering from mastitis in Poland. Scientific World Journal 2012; 2012: 196347. Costa EO, Gandra CR, Pires MF, Coutinho SD, Castilho W, Teixeira CM. Survey of bovine mycotic mastitis in dairy herds in the state of Săo Paulo, Brazil. Mycopathologia 1993; 124: 13- 17. Staroniewicz Z, Włodarczak A, Florek M, Król J. Fungal flora in cows with mastitis and its susceptibility to antimycotics. Med Mycol 2007; 14: 257-259.
  3. Şeker E, Özenç E. In vitro biofilm activity of Candida species isolated from Anatolian buffaloes with mastitis in Western Turkey. Vet Arh 2011; 81: 723-730.
  4. Sartori LCA, Santos RC, Marin JM. Identification of Candida species isolated from cows suffering mastitis in four Brazilian states. Arq Bras Med Vet Zootec 2014; 66: 1615-1617.
  5. Kano R, Hattori Y, Okuzumi K, Miyazaki Y, Yamauchi R, Koie H, Watari T, Hasegawa A. Detection and identification of the Candida species by 25S ribosomal DNA analysis in the urine of candidal cystitis. J Vet Med Sci 2002; 64: 115-117.
  6. Dieffenbach CW, Lowe TMJ, Veksler GS. General concepts for PCR primer design. PCR Methods Appl 1993; 3: 30-37.
  7. Kanbe T, Horii T, Arishima T, Ozeki M, Kikuchi A. PCR-based identification of pathogenic Candida species using primer mixes specific to Candida DNA topoisomerase II genes. Yeast 2002; 19: 973-989.
  8. Kanbe T, Arishima T, Horii T, Kikuchi A. Improvements of PCR-based identification targeting the DNA topoisomerase ii gene to determine major species of the opportunistic fungi Candida and Aspergillus fumigatus. Microbiol Immunol 2003; 47: 631-638.
  9. Kato M, Ozeki M, Kikuchi A, Kanbe T. Phylogenetic relationship and mode of evolution of yeast DNA topoisomerase II gene in the pathogenic Candida species. Gene 2001; 272: 275-281. Krukowski H. Mycotic mastitis in cows. Med Weter 2001; 57: 18-20.
  10. Quinn PJ, Markey B, Carter ME, Donnelly WJ, Leonard FC. Veterinary Microbiology and Microbial Disease. Ames, IA, USA: Blackwell Publishing Professional; 2002. Clinical Laboratory Standards Institute (CLSI). Method for Antifungal Disk Diffusion Susceptibility Testing of Yeasts: Approved Guidelines. Second Edition. CLSI Document M44-A2. Wayne, PA, USA: Clinical Laboratory Standard Institute; 2009.
  11. Eldesouky I, Moha