SavedAdd your journal

Journals / Turkish Journal of Veterinary and Animal Sciences / 2005 / Cilt: 29 - Sayı: 2

Cloning of Nucleoprotein Gene (NP) of Rinderpest Virus (RPV) RBOK Vaccine Strain

Şükrü TONBAK
Aykut ÖZDARENDELİ
Yusuf BOLAT
Pages
297–301
Publication Date
2005-02-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

PCR screening and enzyme digestion assays carried out to verify the presence of the NP gene.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In the present study cloning of the NP gene in a cloning vector is reported.
📊
Key Findings & Data:The NP gene, 1575 bp in length, was cloned into pGEM-3Zf (-) cloning vector.
💡
Conclusion & Impact:PCR screening and enzyme digestion assays carried out to verify the presence of the NP gene.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Rinderpest virus · nucleoprotein gene · cloning

Abstract

In the present study cloning of the NP gene in a cloning vector is reported. For this purpose, Vero cells were infected with rinderpest RBOK vaccine strain. Total RNA was obtained from the infected cells and reverse-transcription (RT) was performed to get cDNAs of NP gene. Afterwards, the NP gene was amplified by polymerase chain reaction (PCR) with the primers specific to the NP gene. The NP gene, 1575 bp in length, was cloned into pGEM-3Zf (-) cloning vector. PCR screening and enzyme digestion assays carried out to verify the presence of the NP gene.

Özet

In the present study cloning of the NP gene in a cloning vector is reported. For this purpose, Vero cells were infected with rinderpest RBOK vaccine strain. Total RNA was obtained from the infected cells and reverse-transcription (RT) was performed to get cDNAs of NP gene. Afterwards, the NP gene was amplified by polymerase chain reaction (PCR) with the primers specific to the NP gene. The NP gene, 1575 bp in length, was cloned into pGEM-3Zf (-) cloning vector. PCR screening and enzyme digestion assays carried out to verify the presence of the NP gene.

🔖

Cite This Article

TONBAK, Ş., ÖZDARENDELİ, A., BOLAT, Y. (2005). Cloning of Nucleoprotein Gene (NP) of Rinderpest Virus (RPV) RBOK Vaccine Strain. Turkish Journal of Veterinary and Animal Sciences, Cilt: 29 - Sayı: 2, 297–301. https://ajindex.com/articles/122365

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (12)

  1. Baron, M.D., Barrett, T.: Rinderpest viruses lacking the C and V proteins show specific defects in growth and transcription of viral RNAs. J. Virol., 2000; 74: 2603-2611.
  2. Kamata, H., Tsukiyama, K., Sugiyama, M., Kamata, Y., Yoshikawa, Y., Yamanouchi, K.: Nucleotide sequence of cDNA to the rinderpest virus mRNA encoding the nucleocapsid protein. Virus Genes, 1991; 5: 5-15.
  3. Ghosh, A., Joshi, V.D., Shaila, M.S.: Characterization of an in vitro transcription system from rinderpest virus. Vet. Microbiol., 1995; 44: 165-173.
  4. Baron, M.D., Barrett, T.: The sequence of the N and L genes of rinderpest virus, and the 5’and 3’extra-genic sequences: The completion of the genome sequence of the virus. Vet. Microbiol., 1995; 44: 175-186.
  5. Fooks, A.R., Schadeck, E., Liebert, U.W., Dowsett, A.B., Rima, B.K., Steward, M., Stephenson, J.R., Wilkinson, G.W.G.: High- level of the measles nucleocapsid protein by using a replication- deficient adenovirus vector: Induction of an MHC-1 restricted CTL response and protection in a murine model. Virology., 1995; 210: 456-465.
  6. Conzelmann, K.K.: Nonsegmented negative-strand RNA viruses: Genetics and manipulation of viral genomes. Annu. Rev. Genet., 1998; 32: 123-162.
  7. Buchholz, C.J., Retzler, C., Homann, H.E., Neubert, W.J.: The carboxy-terminal domain of sendai virus nucleocapsid protein is involved in complex formation between phosphoprotein and nucleocapsid-like particles. Virology., 1994; 204: 770-776.
  8. Curran, J., Homann, H., Buchholz, C., Rochat, S., Neubert, W., Kolakofsky, D.: The hypervariable C-terminal tail of the sendai paramyxovirus nucleocapsid protein is required for template function but not for RNA encapsidation. J. Virol., 1993; 67: 4358-4364.
  9. Ryan, K.W., Portner, A., Murti, K.G.: Antibodies to paramyxovirus nucleoproteins define regions important for immunogenicity and nucleocapsid assembly. Virology., 1993; 193: 376-384.
  10. Ismail, T., Ahmad, S., D’souza-Ault, M., Bassiri, M., Saliki, J., Mebus, C., Yilma, T.: Cloning and expression of the nucleocapsid gene of virulent Kabete O strain of rinderpest virus in baculovirus: Use in differential diagnosis between vaccinated and infected animals. Virology., 1994; 198: 138-147.
  11. Kamata, H., Ohkubo, S., Sugiyama, M., Matsuura, Y., Kamata, Y., Tsukiyama-Kohara, K., Imaoka, K., Kai, C., Yoshikawa, Y., Yamanouchi, K.: Expression in baculovirus vector system of the nucleocapsid protein gene of rinderpest virus. J. Virol. Meth., 1993; 43: 159-166.
  12. Grubman, M.J., Mebus, C., Dale, B., Yam