AI Summary & Key Takeaways
AI InsightThis study revealed that ELISA was the most sensitive test for the detection of B. avium antibodies; however, it was determined that SPAT and AGID test can also be used in screening for B. avium antibodies.
📌 3 Key Takeaways
Abstract
The aims of this study were to develop a serum plate agglutination test (SPAT) antigen and agar gel immunodiffusion (AGID) test antigen for the serological detection of turkeys that have been exposed to Bordetella avium; to compare the sensitivity of commercial enzyme-linked immunosorbent assay (ELISA) with SPAT, and AGID test, and to survey B. avium antibodies in turkey flocks in Aydın, Turkey. For these purposes, serum samples collected from 300 turkeys were examined by ELISA, SPAT, and AGID test. The seroprevalence was 44%, 28%, and 15% for ELISA, SPAT, and AGID test, respectively. This study revealed that ELISA was the most sensitive test for the detection of B. avium antibodies; however, it was determined that SPAT and AGID test can also be used in screening for B. avium antibodies.
Özet
Bu çal›flman›n amaçlar› Bordetella avium’a maruz kalm›fl hindilerin serolojik olarak saptanmas› için serum pleyt aglutinasyon test (SPAT) ve agar jel immunodiffuzyon (AGID) test antijenlerinin gelifltirilmesi, ticari enzimle iflaretli immun deneyi (ELISA) ile SPAT ve AGID testinin duyarl›l›klar›n›n karfl›laflt›r›lmas›, Ayd›n’da hindi sürülerinde B. avium antikorlar›n›n serolojik olarak taranmas›d›r. Bu amaçla, 300 hindiden toplanan serum örnekleri ELISA, SPAT ve AGID test yöntemleriyle incelendi. Seroprevalens ELISA, SPAT ve AGID testi için s›ras›yla % 44, % 28, % 15 olarak belirlendi. Çal›flmada, B. avium antikorlar›n›n tespit edilmesinde en duyarl› testin ELISA oldu¤u; bununla birlikte, SPAT ve AGID testinin de B. avium antikorlar›n›n belirlenmesinde kullan›labilece¤i tespit edildi.