SavedAdd your journal

Journals / Turkish Journal of Veterinary and Animal Sciences / 2013 / Cilt: 37 - Sayı: 1

Effect of oocyte quality and activation protocols on bovine embryo development following intracytoplasmic sperm injection

Ömer KORKMAZ
Şükrü KÜPLÜLÜ
Yüksel AĞCA
İbrahim Mert POLAT
Pages
26–30
Publication Date
2013-01-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, using Ca ionophore + 6-DMAP improves the in vitro developmental competence for bovine embryos after ICSI.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The purpose of this study was to investigate the effects of oocyte quality and activation protocols on the in vitro developmental competence of bovine embryos after intracytoplasmic sperm injection (ICSI).
📊
Key Findings & Data:All of the oocytes were activated using a calcium ionophore only, ethanol only, and 6-dimethylaminopurine (6-DMAP) following calcium ionophore.
💡
Conclusion & Impact:In conclusion, using Ca ionophore + 6-DMAP improves the in vitro developmental competence for bovine embryos after ICSI.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Bovine oocyte · calcium ionophore · ethanol

Abstract

The purpose of this study was to investigate the effects of oocyte quality and activation protocols on the in vitro developmental competence of bovine embryos after intracytoplasmic sperm injection (ICSI). Bovine oocytes were grouped as being of excellent, good, and poor quality. All of the oocytes were activated using a calcium ionophore only, ethanol only, and 6-dimethylaminopurine (6-DMAP) following calcium ionophore. For the excellent quality oocytes, cleavage rates after ICSI were 70% in the Ca ionophore + 6-DMAP activation group, 47% in the ethanol activation group, 41.82% in the Ca ionophore group, and 30% in the nontreated control group. Cleavage rates for the good quality oocytes after activation with these different agents were 31%, 16.03%, 19.8%, and 12.9%, respectively, while the rates for the poor quality oocytes were lower (7%, 6%, 2%, and 1%, respectively). In conclusion, using Ca ionophore + 6-DMAP improves the in vitro developmental competence for bovine embryos after ICSI. Furthermore, the results for the excellent quality oocytes indicate the importance of the preselection of the oocytes and activation methods.

Özet

The purpose of this study was to investigate the effects of oocyte quality and activation protocols on the in vitro developmental competence of bovine embryos after intracytoplasmic sperm injection (ICSI). Bovine oocytes were grouped as being of excellent, good, and poor quality. All of the oocytes were activated using a calcium ionophore only, ethanol only, and 6-dimethylaminopurine (6-DMAP) following calcium ionophore. For the excellent quality oocytes, cleavage rates after ICSI were 70% in the Ca ionophore + 6-DMAP activation group, 47% in the ethanol activation group, 41.82% in the Ca ionophore group, and 30% in the nontreated control group. Cleavage rates for the good quality oocytes after activation with these different agents were 31%, 16.03%, 19.8%, and 12.9%, respectively, while the rates for the poor quality oocytes were lower (7%, 6%, 2%, and 1%, respectively). In conclusion, using Ca ionophore + 6-DMAP improves the in vitro developmental competence for bovine embryos after ICSI. Furthermore, the results for the excellent quality oocytes indicate the importance of the preselection of the oocytes and activation methods.

🔖

Cite This Article

KORKMAZ, Ö., KÜPLÜLÜ, Ş., AĞCA, Y., POLAT, İ. M. (2013). Effect of oocyte quality and activation protocols on bovine embryo development following intracytoplasmic sperm injection. Turkish Journal of Veterinary and Animal Sciences, Cilt: 37 - Sayı: 1, 26–30. https://ajindex.com/articles/122161

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (14)

  1. Gordon, I.: Laboratory Production of Cattle Embryos. 2nd ed., CAB International, Wallingford, UK. 2003.
  2. Hinrichs, K.: Update on equine ICSI and cloning. Theriogenology, 2005; 64: 535–541.
  3. Westhusin, M.E., Anderson, J.G., Harms, P.G., Kraemer, D.C.: Microinjection of spermatozoa into bovine eggs. Theriogenology, 1984; 21: 274. Terada, Y., Nakamura, S., Morita, J., Simerly, C., Hewitson, L., Murakami, T., Yaegashi, N., Schatten, G., Okamura, K.: Intracytoplasmic sperm injection: stiletto conception or a stab in the dark. Arch. Androl., 2003; 49: 169–177.
  4. Terada, Y., Nakamura, S., Morita, J., Tachibana, M., Morita, Y., Ito, K., Murakami, T., Yaegashi, N., Okamura, K.: Use of mammalian eggs for assessment of human sperm function: molecular and cellular analyses of fertilization by intracytoplasmic sperm injection. Am. J. Reprod. Immunol., 2004; 51: 290–293.
  5. Chung, J.T., Keefer, C.L., Downey, B.R.: Activation of bovine oocytes following intracytoplasmic sperm injection (ICSI). Theriogenology, 2000; 53: 273–284.
  6. Keller, D.L.: Oocyte activation and sperm lyophilization: implications for intracytoplasmic sperm injection in the bovine. PhD Dissertation, Texas A&M University, College Station, Texas, USA. 2001.
  7. Malcuit, C., Maserati, M., Takahaski, Y., Page, R., Fissore, R.A.: Intracytoplasmic sperm injection in the bovine induces abnormal [Ca2+] response and oocyte activation. Reprod. Fertil. Dev., 2006; 18: 39–51.
  8. Okitsu, O., Yamano, S., Aono, T.: Activation of bovine oocytes matured in vitro by injection of bovine and human spermatozoa or their cytosolic fractions. Zygote, 2001; 9: 89–95.
  9. Williams, C.J.: Signalling mechanisms of mammalian oocyte activation. Hum. Reprod. Update, 2002; 8(4): 313–321.
  10. Boni, R., Gualtieri, R., Talevi, R., Tosti, E.: Calcium and other ion dynamics during gamete maturation and fertilization. Theriogenology, 2007; 68: 156–164.
  11. Yoo, J.G., Choe, S.Y., Rho, G.J.: Efficient production of cloned bovine embryos using cdc2 kinase inhibitor. Reprod. Domest. Anim., 2003; 38: 444–450.
  12. Brackett, B.G., Zuelke, K.A.: Analysis of factors involved in the in vitro production of bovine embryos. Theriogenology, 1993; 39: 43–64.
  13. Chen, S.H., Seidel, G.E. Jr: Effects of oocyte activation and treatment of spermatozoa on embryonic development following intracytoplasmic sperm injection in cattle. Theriogenology, 1997; 48: 1265–1273.
  14. Oikawa, T., Takada, N., Kikuchi, T., Numabe, T., Takenaka, M., Horiuchi, T.: Evaluation of activation treatments for blastocyst pr