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Journals / Turkish Journal of Veterinary and Animal Sciences / 2007 / Cilt: 31 - Sayı: 4

Coagulase Gene Polymorphism of Staphylococcus aureus Isolated from Subclinical Bovine Mastitis

Özkan ASLANTAŞ
Cemil DEMİR
Hülya TÜRÜTOĞLU
Zafer CANTEKİN
Pages
253–257
Publication Date
2007-04-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results indicated that many coagulase gene genotypes are present in the studied regions and only 1 or 2 genotypes predominated.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study was conducted to investigate the coagulase gene polymorphism of Staphylococcus aureus isolated from subclinical bovine mastitic milk samples from 3 different Turkish cities (Hatay, Gaziantep, and Burdur).
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Key Findings & Data:Amplification of the coagulase gene from 80 S. aureus isolates produced 5 different polymerase chain reaction (PCR) products; 79 isolates showed only 1 amplicon, and 1 isolate showed 2 amplicons.
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Conclusion & Impact:The results indicated that many coagulase gene genotypes are present in the studied regions and only 1 or 2 genotypes predominated.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Staphylococcus aureus · RFLP-PCR · mastitis

Abstract

This study was conducted to investigate the coagulase gene polymorphism of Staphylococcus aureus isolated from subclinical bovine mastitic milk samples from 3 different Turkish cities (Hatay, Gaziantep, and Burdur). Amplification of the coagulase gene from 80 S. aureus isolates produced 5 different polymerase chain reaction (PCR) products; 79 isolates showed only 1 amplicon, and 1 isolate showed 2 amplicons. The isolates were grouped into 9 genotypes by analysing the restriction fragment length polymorphism (RFLP) of the gene; the 2 most common genotypes accounted for 73.8% of the isolates. The results indicated that many coagulase gene genotypes are present in the studied regions and only 1 or 2 genotypes predominated.

Özet

This study was conducted to investigate the coagulase gene polymorphism of Staphylococcus aureus isolated from subclinical bovine mastitic milk samples from 3 different Turkish cities (Hatay, Gaziantep, and Burdur). Amplification of the coagulase gene from 80 S. aureus isolates produced 5 different polymerase chain reaction (PCR) products; 79 isolates showed only 1 amplicon, and 1 isolate showed 2 amplicons. The isolates were grouped into 9 genotypes by analysing the restriction fragment length polymorphism (RFLP) of the gene; the 2 most common genotypes accounted for 73.8% of the isolates. The results indicated that many coagulase gene genotypes are present in the studied regions and only 1 or 2 genotypes predominated.

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Cite This Article

ASLANTAŞ, Ö., DEMİR, C., TÜRÜTOĞLU, H., CANTEKİN, Z. (2007). Coagulase Gene Polymorphism of Staphylococcus aureus Isolated from Subclinical Bovine Mastitis. Turkish Journal of Veterinary and Animal Sciences, Cilt: 31 - Sayı: 4, 253–257. https://ajindex.com/articles/122140

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References (5)

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