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Journals / Turkish Journal of Veterinary and Animal Sciences / 2012 / Cilt: 36 - Sayı: 4

Toxinotyping of Clostridium perfringens isolates by ELISA and PCR from lambs suspected of enterotoxemia

Hasan Hüseyin HADİMLİ*
Osman ERGANİŞ
Zafer SAYIN
Zeki ARAS
Pages
409–415
Publication Date
2012-04-01
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

The results showed that C. perfringens type A was the most common type by both ELISA and PCR in lambs with enterotoxemia.

📌 3 Key Takeaways

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Objective & Hypothesis:Clostridium perfringens is a gram-positive, anaerobic bacterium that causes a wide range of diseases in humans and animals.
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Key Findings & Data:The purposes of this study were to determine, using the enzyme-linked immunosorbent assay (ELISA), the clostridial toxins in intestinal samples of lambs with suspected enterotoxemia; to conduct molecular typing of C. perfringens isolates; and to investigate the presence of the genes of 4 major toxins (a, b, e, and i) in the isolates by polymerase chain reaction (PCR).
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Conclusion & Impact:The results showed that C. perfringens type A was the most common type by both ELISA and PCR in lambs with enterotoxemia.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Clostridium perfringens · lamb · enterotoxemia

Abstract

Clostridium perfringens is a gram-positive, anaerobic bacterium that causes a wide range of diseases in humans and animals. The purposes of this study were to determine, using the enzyme-linked immunosorbent assay (ELISA), the clostridial toxins in intestinal samples of lambs with suspected enterotoxemia; to conduct molecular typing of C. perfringens isolates; and to investigate the presence of the genes of 4 major toxins (a, b, e, and i) in the isolates by polymerase chain reaction (PCR). According to the ELISA results, clostridial toxins were determined in 32 intestinal samples. Of the samples, 13 (40.62%) were C. perfringens type A, 1 (3.125%) was C. perfringens type B, 9 (28.125%) were C. perfringens type C, and 9 (28.125%) were C. perfringens type D. C. perfringens was isolated from 13 (8.66%) out of 150 intestinal samples. A total of 13 C. perfringens isolates from lambs were genotyped by PCR. Of the isolates, 10 (76.92%) were type A, 2 (%15.38) were type D, and 1 (7.69%) was type C. Type B and type E were not identified. The results showed that C. perfringens type A was the most common type by both ELISA and PCR in lambs with enterotoxemia.

Özet

Clostridium perfringens is a gram-positive, anaerobic bacterium that causes a wide range of diseases in humans and animals. The purposes of this study were to determine, using the enzyme-linked immunosorbent assay (ELISA), the clostridial toxins in intestinal samples of lambs with suspected enterotoxemia; to conduct molecular typing of C. perfringens isolates; and to investigate the presence of the genes of 4 major toxins (a, b, e, and i) in the isolates by polymerase chain reaction (PCR). According to the ELISA results, clostridial toxins were determined in 32 intestinal samples. Of the samples, 13 (40.62%) were C. perfringens type A, 1 (3.125%) was C. perfringens type B, 9 (28.125%) were C. perfringens type C, and 9 (28.125%) were C. perfringens type D. C. perfringens was isolated from 13 (8.66%) out of 150 intestinal samples. A total of 13 C. perfringens isolates from lambs were genotyped by PCR. Of the isolates, 10 (76.92%) were type A, 2 (%15.38) were type D, and 1 (7.69%) was type C. Type B and type E were not identified. The results showed that C. perfringens type A was the most common type by both ELISA and PCR in lambs with enterotoxemia.

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Cite This Article

HADİMLİ*, H. H., ERGANİŞ, O., SAYIN, Z., ARAS, Z. (2012). Toxinotyping of Clostridium perfringens isolates by ELISA and PCR from lambs suspected of enterotoxemia. Turkish Journal of Veterinary and Animal Sciences, Cilt: 36 - Sayı: 4, 409–415. https://ajindex.com/articles/122063

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