SavedAdd your journal

Journals / Turkish Journal of Veterinary and Animal Sciences / 2013 / Cilt: 37 - Sayı: 5

Evaluation of canine anti-Leishmania IgG subclasses and their relation with skin signs in naturally infected dogs in the northwest of Iran

Ali Shabestari ASL
Majid SHARIFI
Mehdi MOHEBALI
Zabihollah ZAREI
Zohreh BABALOO
Behnaz AKHOUNDI
Pages
512–515
Publication Date
2013-05-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It seems that measurements of IgG subclasses can be used as gold-standard markers for evaluation of susceptibility or resistance to this lethal chronic infectious disease in dogs.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Canine leishmaniasis (CanL) is a severe, intracellular parasitic zoonosis and is an endemic complaint in Iran.
📊
Key Findings & Data:The aims of this study were to evaluate the relation between anti-Leishmania antibody titers (IgG) and skin symptoms of CanL in naturally affected dogs and to assess the correlation of IgG1 and IgG2 subclasses of titers with these symptoms.
💡
Conclusion & Impact:It seems that measurements of IgG subclasses can be used as gold-standard markers for evaluation of susceptibility or resistance to this lethal chronic infectious disease in dogs.
🔬Design:Research Article
📍Scope:45 samples
⚙️Variables:Key words: Canine leishmaniasis · immunoglobulin G · direct agglutination test

Abstract

Canine leishmaniasis (CanL) is a severe, intracellular parasitic zoonosis and is an endemic complaint in Iran. Dogs are considered the reservoir host for Leishmania infantum in the Old World. The aims of this study were to evaluate the relation between anti-Leishmania antibody titers (IgG) and skin symptoms of CanL in naturally affected dogs and to assess the correlation of IgG1 and IgG2 subclasses of titers with these symptoms. Anti-Leishmania antibody titers were evaluated in 100 dogs' blood samples with the direct agglutination test (DAT) and enzyme-linked immunosorbent assay (ELISA) methods. Based on DAT results, 45 samples were positive. Twenty-five of the positive dogs had cutaneous signs of leishmaniasis and 20 of them were asymptomatic (DAT of 1:320 and above). In the dogs, high levels of total IgG were found in symptomatic dogs. In ELISA, we found an apparent correlation between IgG1 and symptomatic dogs and between IgG2 and asymptomatic dogs. It seems that measurements of IgG subclasses can be used as gold-standard markers for evaluation of susceptibility or resistance to this lethal chronic infectious disease in dogs.

Özet

Canine leishmaniasis (CanL) is a severe, intracellular parasitic zoonosis and is an endemic complaint in Iran. Dogs are considered the reservoir host for Leishmania infantum in the Old World. The aims of this study were to evaluate the relation between anti-Leishmania antibody titers (IgG) and skin symptoms of CanL in naturally affected dogs and to assess the correlation of IgG1 and IgG2 subclasses of titers with these symptoms. Anti-Leishmania antibody titers were evaluated in 100 dogs' blood samples with the direct agglutination test (DAT) and enzyme-linked immunosorbent assay (ELISA) methods. Based on DAT results, 45 samples were positive. Twenty-five of the positive dogs had cutaneous signs of leishmaniasis and 20 of them were asymptomatic (DAT of 1:320 and above). In the dogs, high levels of total IgG were found in symptomatic dogs. In ELISA, we found an apparent correlation between IgG1 and symptomatic dogs and between IgG2 and asymptomatic dogs. It seems that measurements of IgG subclasses can be used as gold-standard markers for evaluation of susceptibility or resistance to this lethal chronic infectious disease in dogs.

🔖

Cite This Article

ASL, A. S., SHARIFI, M., MOHEBALI, M., ZAREI, Z., BABALOO, Z., AKHOUNDI, B. (2013). Evaluation of canine anti-Leishmania IgG subclasses and their relation with skin signs in naturally infected dogs in the northwest of Iran. Turkish Journal of Veterinary and Animal Sciences, Cilt: 37 - Sayı: 5, 512–515. https://ajindex.com/articles/121883

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (13)

  1. References Baneth, G., Itamar, A.: Canine leishmaniasis: a diagnostic and clinical challenge. Vet. J., 2008; 175: 14–15.
  2. Moreno, J., Alvar, J.: Canine leishmaniasis: epidemiological risk and the experimental model. Trends Parasitol., 2002; 18: 399–405.
  3. Mohebali, M., Hamzavim, Y., Zarei, Z.: Study on canine visceral leishmaniasis in some parts of Iran and its health importance. J. Tehran Univ. Vet. Facul., 2001; 56: 55–59.
  4. Alborzi, A., Pouladfar, G.R., Aelami, M.H.: Visceral leishmaniasis; literature review and Iranian experience. Iranian J. Clin. Infect. Dis., 2007; 2: 99–108.
  5. Lappin, M.R.: Protozoal infections. In: Ettinger, S.J., Feldman, E.C., Eds. Textbook of Veterinary Internal Medicine. 7th edn., Saunders, St. Louis. 2010; 915–916.
  6. Oliva, G., Scalone, A., Manzillo, V.F., Gramiccia, M., Pagano, A., Di Muccio, T., Gradoni, L.: Incidence and time course of Leishmania infantum infections examined by parasitological serologic, and nested-PCR techniques in a cohort of naive dogs exposed to three consecutive transmission seasons. J. Clin. Microbiol., 2006; 44: 1318–1322.
  7. Özensoy Töz, S., Özbel, Y., Ertabaklar, H., Yıldızlı, N., Korkmaz, M, Alkan, M.Z.: Comparisons of clinical findings and serological data in the diagnosis of canine leishmaniosis. Turk. J. Vet. Anim. Sci., 2005; 29: 269–273.
  8. El Harith, A., Slappendel, R.J., Reiter, I., Van Knapen, F., De Korte, P., Huigen, E., Kolk, A.H.: Application of a direct agglutination test for detection of specific anti-Leishmania antibodies in the canine reservoir. J. Clin. Microbiol., 1989; 27: 2252–2257.
  9. Carrera, L., Fermín, M.L., Tesouro, M., Garcia, P., González, J.L., Mendez, S., Cuquerella, M., Alunda, J.M.: Antibody response in dogs experimentally infected with L. infantum infection course antigen markers. Exp. Parasitol., 1996; 82: 139–146.
  10. Riera, C., Valladares, J.E., Gállego, M., Aisa, M.J., Castillejo, S., Fisa, R., Ribas, N., Carrio, J., Alberola, J., Arboix, M.: Serological and parasitological follow-up in dogs experimentally infected with L. infantum and treated with meglumine antimoniate. Vet. Parasitol., 1999; 84: 33–47.
  11. Gradoni, L., Gramiccia, M.: Leishmaniasis. OIE Terrestrial Manual, 2008; 2.1.8: 241–250.
  12. Pinelli, E., Killick-Kendrick, R., Wagenaar, J., Bernadina, W., del Real, G., Ruitenberg, J.: Cellular and humoral immune responses in dogs, experimentally and naturally infected with L. infantum. Infect. Immun., 1994; 62: 229–235.
  13. Iniesta, L., Gállego, M., Portşs, M.: Immunoglobulin G and E resp