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Journals / Turkish Journal of Veterinary and Animal Sciences / 2013 / Cilt: 37 - Sayı: 4

A survey study on hantavirus, cowpox virus, and Leptospira infections in Microtus hartingi in Kırşehir Province, Central Anatolia, Turkey

Ahmet Kürşat AZKUR
Sedat KAYGUSUZ
Muhammet Eren ASLAN
Pages
434–442
Publication Date
2013-04-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Therefore, additional studies are needed.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The aim of this study was to investigate whether hantaviruses, cowpox viruses, or Leptospira infections were prevalent in Microtus hartingi trapped in Kırşehir Province, located in Central Anatolia, Turkey.
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Key Findings & Data:Leptospira spp. was detected in 20 of the 43 (46.5%) Microtus hartingi kidney samples and confirmed by nested polymerase chain reaction (PCR) of the 16S ribosomal RNA gene.
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Conclusion & Impact:Therefore, additional studies are needed.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:hantavirus · cowpox virus · Leptospira spp.

Abstract

The aim of this study was to investigate whether hantaviruses, cowpox viruses, or Leptospira infections were prevalent in Microtus hartingi trapped in Kırşehir Province, located in Central Anatolia, Turkey. Leptospira spp. was detected in 20 of the 43 (46.5%) Microtus hartingi kidney samples and confirmed by nested polymerase chain reaction (PCR) of the 16S ribosomal RNA gene. Five of the 20 Leptospira spp. PCR positive amplicons were sequenced, analyzed, and confirmed as Leptospira spp. This is the first study to genetically characterize Leptospira in Microtus hartingi in Turkey. There was no evidence of cowpox viruses or hantaviruses in Microtus hartingi. Therefore, additional studies are needed.

Özet

The aim of this study was to investigate whether hantaviruses, cowpox viruses, or Leptospira infections were prevalent in Microtus hartingi trapped in Kırşehir Province, located in Central Anatolia, Turkey. Leptospira spp. was detected in 20 of the 43 (46.5%) Microtus hartingi kidney samples and confirmed by nested polymerase chain reaction (PCR) of the 16S ribosomal RNA gene. Five of the 20 Leptospira spp. PCR positive amplicons were sequenced, analyzed, and confirmed as Leptospira spp. This is the first study to genetically characterize Leptospira in Microtus hartingi in Turkey. There was no evidence of cowpox viruses or hantaviruses in Microtus hartingi. Therefore, additional studies are needed.

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Cite This Article

AZKUR, A. K., KAYGUSUZ, S., ASLAN, M. E. (2013). A survey study on hantavirus, cowpox virus, and Leptospira infections in Microtus hartingi in Kırşehir Province, Central Anatolia, Turkey. Turkish Journal of Veterinary and Animal Sciences, Cilt: 37 - Sayı: 4, 434–442. https://ajindex.com/articles/121727

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References (7)

  1. Ahmed, N., Devi, S.M., De los Á Valverde, M., Vijayachari, P., Machang’u, R.S., Ellis, W.A., Hartskeerl, R.A.: Multilocus sequence typing method for identification and genotypic classification of pathogenic Leptospira species. Ann. Clin. Microbiol. Antimicrob., 2006; 5: 28. Weisburg, W.G., Barns, S.M., Pelletier, D.A., Lane, D.J.: 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol., 1991; 173: 697–703.
  2. Dutta, T.K., Christopher, M.: Leptospirosis—an overview. J. Assoc. Physicians India, 2005; 53: 545–551.
  3. Ganoza, C.A., Matthias, M.A., Collins-Richards, D., Brouwer, K.C., Cunningham, C.B., Segura, E.R., Gilman, R.H., Gotuzzo, E., Vinetz, J.M.: Determining risk for severe leptospirosis by molecular analysis of environmental surface waters for pathogenic Leptospira. PLoS Med., 2006; 3: 308. Ko, A.I., Goarant, C., Picardeau, M.: Leptospira: the dawn of the molecular genetics era for an emerging zoonotic pathogen. Nat. Rev. Microbiol., 2009; 7: 736–747.
  4. Matthias, M.A., Ricaldi, J.N., Cespedes, M., Diaz, M.M., Galloway, R.L., Saito, M., Steigerwalt, A.G., Patra, K.P., Ore, C.V., Gotuzzo, E., Gilman, R.H., Levett, P.N., Vinetz, J.M.: Human leptospirosis caused by a new, antigenically unique Leptospira associated with a Rattus species reservoir in the Peruvian Amazon. PLoS Negl. Trop. Dis., 2008; 2: e213. Djadid, N.D., Ganji, Z.F., Gouya, M.M., Rezvani, M., Zakeri, S.: A simple and rapid nested polymerase chain reactionrestriction fragment length polymorphism technique for differentiation of pathogenic and nonpathogenic Leptospira spp. Diagn. Microbiol. Infect. Dis., 2009; 63: 251–256.
  5. Seixas, L.D.S., de Melo, C.B., Leite, R.C., Moreira, É.C., McManus, C.M., de Castro, M.B.: Anti-Leptospira spp. agglutinins in ewes in the Federal District, Brazil. Trop. Anim. Health Prod.: 2011: 43; 9–11. World Health Organization. Leptospirosis worldwide. Weekly epidemiological record, 2001: 76; 109–116.
  6. Laakkonen, J., Kallio-Kokko, H., Öktem, M.A., Blasdell, K., Plyusnina, A., Niemimaa, J., Karataş, A., Plyusnin, A., Vaheri, A., Henttonen, H.: Serological survey for viral pathogens in Turkish rodents. J. Wildl. Dis., 2006; 42: 672–676. Mérien, F., Amouriaux., P., Perolat, P., Baranton, G., Saint Girons, I.: Polymerase chain reaction for detection of Leptospira spp. in clinical samples. J. Clin. Microbiol., 1992; 30: 2219–2224.
  7. Serif, H.: The morbus well cases Istanbul. Istanbul seririyati, 1922; 2: 101. Bulu, A.A., Dörterler, R., Özkan, O., Hoştürk, F.: Doğu An