SavedAdd your journal

Journals / Turkish Journal of Veterinary and Animal Sciences / 2013 / Cilt: 37 - Sayı: 4

Glycohistochemistry of the lateral tympanic membrane in the syrinx of the Denizli cock

Nazan KESKİN
Pınar İLİ
Pages
414–418
Publication Date
2013-04-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These results suggest that LTM exhibits an intense reactivity with AB-pH 2.5, indicating carboxylated carbohydrates, and it also has terminal saccharide moieties consisting of b(1->3) linked galactose, a(2->3) linked sialic acid, mannose, b (1->4) linked galactose, and a (2->6) linked sialic acid.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The Denizli cock is a special race that has harmonious long crowing.
📊
Key Findings & Data:Paraffin sections were stained with Alcian blue (AB) at pH 1 and pH 2.5 for demonstration of sulfated and carboxylated carbohydrates, respectively.
💡
Conclusion & Impact:These results suggest that LTM exhibits an intense reactivity with AB-pH 2.5, indicating carboxylated carbohydrates, and it also has terminal saccharide moieties consisting of b(1->3) linked galactose, a(2->3) linked sialic acid, mannose, b (1->4) linked galactose, and a (2->6) linked sialic acid.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Denizli cock · syrinx · tympanic membrane

Abstract

The Denizli cock is a special race that has harmonious long crowing. In this study, the syrinx of the Denizli cock was studied by using glycohistochemical analyses. Paraffin sections were stained with Alcian blue (AB) at pH 1 and pH 2.5 for demonstration of sulfated and carboxylated carbohydrates, respectively. At AB-pH 1, the lateral tympanic membrane (LTM) and also the medial tympanic membrane (MTM) exhibited a weak reactivity, whereas they were stained intensely at AB-pH 2.5. The frozen sections were incubated with 5 digoxigenin-labeled lectins for lectin histochemistry: peanut agglutinin, Maackia amurensis leucoagglutinin, Galanthus nivalis agglutinin, Datura stramonium agglutinin, and Sambucus nigra agglutinin. In LTM, positive stainings were observed for the 5 lectins. These results suggest that LTM exhibits an intense reactivity with AB-pH 2.5, indicating carboxylated carbohydrates, and it also has terminal saccharide moieties consisting of b(1->3) linked galactose, a(2->3) linked sialic acid, mannose, b (1->4) linked galactose, and a (2->6) linked sialic acid.

Özet

The Denizli cock is a special race that has harmonious long crowing. In this study, the syrinx of the Denizli cock was studied by using glycohistochemical analyses. Paraffin sections were stained with Alcian blue (AB) at pH 1 and pH 2.5 for demonstration of sulfated and carboxylated carbohydrates, respectively. At AB-pH 1, the lateral tympanic membrane (LTM) and also the medial tympanic membrane (MTM) exhibited a weak reactivity, whereas they were stained intensely at AB-pH 2.5. The frozen sections were incubated with 5 digoxigenin-labeled lectins for lectin histochemistry: peanut agglutinin, Maackia amurensis leucoagglutinin, Galanthus nivalis agglutinin, Datura stramonium agglutinin, and Sambucus nigra agglutinin. In LTM, positive stainings were observed for the 5 lectins. These results suggest that LTM exhibits an intense reactivity with AB-pH 2.5, indicating carboxylated carbohydrates, and it also has terminal saccharide moieties consisting of b(1->3) linked galactose, a(2->3) linked sialic acid, mannose, b (1->4) linked galactose, and a (2->6) linked sialic acid.

🔖

Cite This Article

KESKİN, N., İLİ, P. (2013). Glycohistochemistry of the lateral tympanic membrane in the syrinx of the Denizli cock. Turkish Journal of Veterinary and Animal Sciences, Cilt: 37 - Sayı: 4, 414–418. https://ajindex.com/articles/121717

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (16)

  1. Hodges, R.D.: The Histology of the Fowl. Academic Press, London. 1974.
  2. Brakenbury, J.: Control of sound production in the syrinx of the fowl Gallus gallus. J. Exp. Biol., 1980; 85: 239–251.
  3. Gaunt, A.S., Gaunt, S.L.L.: Mechanics of the syrinx in Gallus gallus. 11. Electromyographic studies of ad libitum vocalizations. J. Morph., 1977; 152: 1–20.
  4. Goller, F., Larsen, O.N.: In situ biomechanics of the syrinx and sound generation in pigeons. J. Exp. Biol., 1997; 200: 2165– 21 Goller, F., Larsen, O.N.: Role of syringeal vibrations in bird vocalizations. Proc. R. Soc. Lond. B, 1999; 266: 1609–1615.
  5. Larsen, O.N., Goller, F.: Direct observation of syringeal muscle function in song birds and a parrot. J. Exp. Biol., 2002; 205: 25– Larsen, O.N., Goller, F., van Leeuwen, J.L.: Aspects of syringeal mechanics in avian phonation. Acta Zool. Sinica, 2006; 52 (Supplement): 478–481.
  6. Frank, T., Walter, I., Probst, A., König, H.E.: Histological aspects of the syrinx of the male mallard (Anas platyrhynchos). Anat. Histol. Embryol., 2006; 35: 396–401.
  7. Suthers, R.A., Goller, F., Pytte, C.: The neuromuscular control of birdsong. Phil. Trans. R. Soc. Lond. B, 1999; 354: 927–939.
  8. Suthers, R.A.: Peripheral vocal mechanisms in birds: Are songbirds special? Neth. J. Zool., 2001; 51: 217–242.
  9. Goller, F., Larsen, O.N.: A new mechanism of sound generation in songbirds. Proc. Natl. Acad. Sci. USA, 1997; 94: 14787– 147 Riede, T., Goller, F.: Functional morphology of the soundgenerating labia in the syrinx of two song birds species. J. Anat., 2010; 216: 23–36.
  10. Varki, A., Cummings, R., Esko, J., Freze, H., Hart, G., Marth, J.: Essentials of Glycobiology. Cold Spring Harbor Laboratory Press,
  11. Cold Spring Harbor, New York. 1999.
  12. Cerri, P.S., Sasso-Cerri, E.: Staining methods applied to glycol methacrylate embedded tissue sections. Micron, 2003; 34: 365–372.
  13. Lev, R., Spicer, S.S.: Specific staining of sulphate groups with alcian blue at low pH. J. Histochem. Cytochem., 1964; 12: 309. Mowry, R.W.: Alcian blue techniques for the histochemical study of acidic carbohydrates. J. Histochem. Cytochem., 1956; 4: 407–408.
  14. Cevik-Demirkan, A., Ozdemir, V.: A study on anatomical structures of the larynx, trachea and syrinx in Eurasian eagle owl (Bubo bubo). J. Anim. Vet. Adv., 2011; 10: 3218–3224.
  15. Frank, T., Probst, A., König, H.E., Walter, I.: The syrinx of the male mallard (Anas platyrhynchos): special anatomical features. Anat. Histol. Embryol., 2007; 36: 121–126.
  16. Gaban-Lima, R., Höfling, E.: Comparative