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Journals / Turkish Journal of Veterinary and Animal Sciences / 2014 / Cilt: 38 - Sayı: 2

Caspase-1, -2, and -3 gene expression is enhanced in the heart and lung of chickens with pulmonary hypertension (ascites)

Hossein HASSANPOUR
Masood TESHFAM
Hassan MOMTAZ
Hamed ZAREI
Shahab BAHADORAN
Pages
133–137
Publication Date
2014-05-18
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

It is speculated that increased mRNA levels of caspase in the lungs and hearts of pulmonary hypertensive broilers could be evidence of upregulated apoptosis, which is involved in the pathophysiology of broiler chickens with pulmonary hypertension.

📌 3 Key Takeaways

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Objective & Hypothesis:To investigate the involvement of apoptosis in the pathogenesis of T3-induced pulmonary hypertension in broiler chickens, caspase-1, -2, and -3 gene expressions were evaluated by semiquantitative RT-PCR in the lungs and left and right heart ventricles.
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Key Findings & Data:One-day-old fast-growing chickens were randomly divided into 2 equal groups with 3 replicates per group.
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Conclusion & Impact:It is speculated that increased mRNA levels of caspase in the lungs and hearts of pulmonary hypertensive broilers could be evidence of upregulated apoptosis, which is involved in the pathophysiology of broiler chickens with pulmonary hypertension.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Caspase · apoptosis · broiler

Abstract

To investigate the involvement of apoptosis in the pathogenesis of T3-induced pulmonary hypertension in broiler chickens, caspase-1, -2, and -3 gene expressions were evaluated by semiquantitative RT-PCR in the lungs and left and right heart ventricles. One-day-old fast-growing chickens were randomly divided into 2 equal groups with 3 replicates per group. Chicks were reared for 7 weeks under standard conditions. T3 as a thyroid hormone was added to the ration after week 1 of rearing. Pulmonary hypertension was induced at 49 days based on RV/TV ratio index. The caspase genes were constitutively expressed in the lung and right and left ventricles of intact and pulmonary hypertensive broiler chickens. The relative amount of caspase-1, -2, and -3 mRNA in the lung and right and left ventricles of the heart was higher in pulmonary hypertensive broilers than in control broilers (P < 0.05). It is speculated that increased mRNA levels of caspase in the lungs and hearts of pulmonary hypertensive broilers could be evidence of upregulated apoptosis, which is involved in the pathophysiology of broiler chickens with pulmonary hypertension.

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Cite This Article

HASSANPOUR, H., TESHFAM, M., MOMTAZ, H., ZAREI, H., BAHADORAN, S. (2014). Caspase-1, -2, and -3 gene expression is enhanced in the heart and lung of chickens with pulmonary hypertension (ascites). Turkish Journal of Veterinary and Animal Sciences, Cilt: 38 - Sayı: 2, 133–137. https://ajindex.com/articles/121607

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