The relationship between seropositivity and tissue cysts in sheep naturally infected with Toxoplasma gondii

The relationship between the presence of tissue cysts and seropositivity in sheep was statistically significant at 1/16 (P < 0.01) and at 1/64 and 1/128 (P < 0.001) serum dilutions.

📌 3 Key Takeaways

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Objective & Hypothesis:Skeletal muscles (tongue, masseter, leg, intercostal, and diaphragmatic muscles) and brain samples of 100 sheep at slaughter were analysed for the presence of T. gondii tissue cysts along with serum IgG titres.

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Key Findings & Data:Seropositivity was detected in 88% (88/100) of sheep sera analysed by indirect fluorescent antibody test.

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Conclusion & Impact:The relationship between the presence of tissue cysts and seropositivity in sheep was statistically significant at 1/16 (P < 0.01) and at 1/64 and 1/128 (P < 0.001) serum dilutions.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Key words: Toxoplasma gondii · tissue cyst · percoll gradient centrifugation

Abstract

Skeletal muscles (tongue, masseter, leg, intercostal, and diaphragmatic muscles) and brain samples of 100 sheep at slaughter were analysed for the presence of T. gondii tissue cysts along with serum IgG titres. Two methods of isolation by percoll gradient centrifugation and tissue microarray (TMA) technique with immunoperoxidase staining were used. Seropositivity was detected in 88% (88/100) of sheep sera analysed by indirect fluorescent antibody test. Tissue cysts were observed in 46 (52.3%, 46/88) and 15 (17%, 15/88) of the seropositive sheep with the isolation technique and TMA and immunoperoxidase staining, respectively. The diameters of the tissue cysts were 25–58 × 25–62 (mean 34 × 36) µm. The relationship between the presence of tissue cysts and seropositivity in sheep was statistically significant at 1/16 (P < 0.01) and at 1/64 and 1/128 (P < 0.001) serum dilutions.

Özet

Skeletal muscles (tongue, masseter, leg, intercostal, and diaphragmatic muscles) and brain samples of 100 sheep at slaughter were analysed for the presence of T. gondii tissue cysts along with serum IgG titres. Two methods of isolation by percoll gradient centrifugation and tissue microarray (TMA) technique with immunoperoxidase staining were used. Seropositivity was detected in 88% (88/100) of sheep sera analysed by indirect fluorescent antibody test. Tissue cysts were observed in 46 (52.3%, 46/88) and 15 (17%, 15/88) of the seropositive sheep with the isolation technique and TMA and immunoperoxidase staining, respectively. The diameters of the tissue cysts were 25–58 × 25–62 (mean 34 × 36) µm. The relationship between the presence of tissue cysts and seropositivity in sheep was statistically significant at 1/16 (P < 0.01) and at 1/64 and 1/128 (P < 0.001) serum dilutions.

Keywords

Key words: Toxoplasma gondii tissue cyst percoll gradient centrifugation tissue microarray serology sheep Turkey

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Cite This Article

YILDIZ, K., KUL, O., GÖKPINAR, S., ATMACA, H. T., GENÇAY, Y. E., GAZYAĞCI, A. N., BABÜR, C., GÜRCAN, İ. S. (2014). The relationship between seropositivity and tissue cysts in sheep naturally infected with Toxoplasma gondii. Turkish Journal of Veterinary and Animal Sciences, Cilt: 38 - Sayı: 2, 169–175. https://ajindex.com/articles/121605

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References (12)

Dubey JP. Toxoplasmosis of Animals and Humans. 2nd edition, CRC Press;
Boca Raton, FL, USA; 2010.
Hill DE, Chirukandoth S, Dubey JP. Biology and epidemiology of Toxoplasma gondii in man and animals. Anim Health Res Rev 2005; 6: 41–61.
Weiss LM, Kim K. Toxoplasma gondii, The Model Apicomplexan: Perspectives and Methods. Academic Press; Amsterdam, the Netherlands; 2007.
Buxton D, Maley SW, Wright SE, Rodger S, Bartley P, Innes EA. Toxoplasma gondii and ovine toxoplasmosis: new aspects of an old story. Vet Parasitol 2007; 149: 25–28.
Hiepe T, Lucius R, Gottstein B. Allgemeine Parasitologie mit den Grundzügen der Immunologie, Diagnostik und Bekämpfung. Parey; Germany, 2006 (in German). Kijlstra A, Jongert E. Toxoplasma-safe meat: close to reality? Trend Parasitol 2008; 25: 18–22.
Slifko TR, Smith HV, Rose JB. Emerging parasite zoonoses associated with water and food. Int J Parasitol 2000; 30: 1379– 13 EFSA, European Food Safety Authority. Scientific opinion of the panel on biological hazards on a request from EFSA on surveillance and monitoring of Toxoplasma in humans, foods and animals. The EFSA Journal 2007; 583: 1–64.
Ortega-Mora LM, Gottstein B, Conraths FJ, Buxton D. Protozoal Abortion in Farm Ruminants: Guidelines for Diagnosis and Control. CAB International; Wallington, UK; 2007.
Yıldız K, Babür C, Kılıç S, Aydenizöz M, Dalkılıç İ. Kırıkkale Mezbahası’nda kesilen koyun ve sığırlar ile mezbaha çalışanlarında anti-Toxoplasma antikorlarının araştırılması. Turkiye Parazitol Derg 2000; 24: 180–185 (article in Turkish with an English abstract). Liu Q, Ma R, Zhao Q, Shang L, Cai J, Wang X, Li J, Hu G, Jin H, Gao H. Seroprevalence of Toxoplasma gondii infection in Tibetan sheep in northwestern China. J Parasitol 2010; 96: 1222–1223.
Dempster RP, Wilkins M, Green RS, de Lisle GW. Serological survey of Toxoplasma gondii and Campylobacter fetus fetus in sheep from New Zealand. N Z Vet J 2011; 59: 155–159.
Hutchinson JP, Wear AR, Lambton SL, Smith RP, Pritchard GC. Survey to determine the seroprevalence of Toxoplasma gondii infection in British sheep flocks. Vet Rec 2011; 169: 582. Blewett DA, Miller JK, Harding J. Simple technique for the direct isolation of toxoplasma tissue cysts from fetal ovine brain. Vet Rec 1983; 112: 98–100.
Gencay YE, Yıldız K, Gökpınar S, Leblebicier A. A potential infection source for humans: frozen buffalo meat can harbour tissue cysts of Toxoplasma gondii. Food Control 2013; 30: 86– Eggleston TL, Fitzpatrick E, Hager KM. Parasitology a