SavedAdd your journal

Journals / Turkish Journal of Veterinary and Animal Sciences / 2018 / Cilt: 42 - Sayı: 4

Evaluation of PCR primers targeting the VP2 region of the foot-and-mouth disease virus for improved serotype detection

Waqas ALI
Mudasser HABIB
Muhammad SALAH UD DIN SHAH
Pages
335–345
Publication Date
2018-08-09
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, this new method offers an improved approach for the serotyping of FMDV compared to previous methods.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Frequently, a sequence from a single strain of a virus is used to design primers for PCR-based virus detection.
📊
Key Findings & Data:However, high mutation rates in RNA viruses lead to failure of microorganism detection in clinical samples.
💡
Conclusion & Impact:In conclusion, this new method offers an improved approach for the serotyping of FMDV compared to previous methods.
🔬Design:Research Article
📍Scope:2010 Verileri
⚙️Variables:Serotyping · foot-and-mouth disease · Pakistan

Abstract

Frequently, a sequence from a single strain of a virus is used to design primers for PCR-based virus detection. However, high mutation rates in RNA viruses lead to failure of microorganism detection in clinical samples. Therefore, it is essential to find conserved regions within sequences to design primers for the detection of pathogen genomes in suspected clinical samples. The aim of the study was to find conserved regions of the genome for improved serotype detection. In the present study, primers were designed for serotype detection based on the VP2 coding region of foot-and-mouth disease virus (FMDV) after alignment of full genome (n = 375) sequences. These primer pairs and previously reported primer pairs were then compared using positive samples (n = 91) collected during 2010? 2016 from Faisalabad District, Pakistan. The detection rate of newly designed primer pairs O-Wqs-F/Rev-Wqs (96.5%), As-Wqs-F/Rev- Wqs (90.4%), and A-Wqs-F/Rev-Wqs (100%) was better compared with previously reported primer pairs P38/P33 (81%), P74?77/P33 (47.6%), and P87?92/P33 (41.6%) in detecting FMDV serotypes O, Asia1, and A, respectively. The higher detection rates of the newly designed primer pairs appeared to be due to the selection of highly conserved sequences within the serotypes for designing primers. To the best of the authors? knowledge, this is the first study that describes serotype diagnosis of FMDV based on primers targeting the VP2 region. In conclusion, this new method offers an improved approach for the serotyping of FMDV compared to previous methods.

🔖

Cite This Article

ALI, W., HABIB, M., SHAH, M. S. U. D. (2018). Evaluation of PCR primers targeting the VP2 region of the foot-and-mouth disease virus for improved serotype detection. Turkish Journal of Veterinary and Animal Sciences, Cilt: 42 - Sayı: 4, 335–345. https://ajindex.com/articles/121462

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (10)

  1. Alexandersen S, Mowat N. Foot-and-mouth disease: host range and pathogenesis. In: Mahy BW, editor. Foot-and- Mouth Disease Virus. Current Topics in Microbiology and Immunology, Vol. 288. Berlin, Heidelberg: Springer; 2005. pp. 9-42.
  2. Clavijo A, Wright P, Kitching P. Developments in diagnostic techniques for differentiating infection from vaccination in foot-and-mouth disease. Vet J 2004; 167: 9-22.
  3. Ali W, Habib M, Khan RSA, Zia MA, Mazhar MU, Javed A, Farooq M, Shah MS. P1 coding region diversity of Group VII (Sind-08) serotype Asia-1 foot-and-mouth disease virus. Kafkas Univ Vet Fak 2018; 24: 341-347. Carrillo C. Foot and mouth disease virus genome. In: Garcia M, editor. Viral Genomes. Rijeka, Croatia: InTech; 2012. pp. 53-68.
  4. Lee KN, Nguyen T, Kim SM, Park JH, Do HT, Ngo HT, Mai DT, Lee SY, Nguyen CV, Yoon SH. Direct typing and molecular evolutionary analysis of field samples of foot-and-mouth disease virus collected in Viet Nam between 2006 and 2007. Vet Micro 2011; 147: 244-252.
  5. Kitching R, Knowles N, Samuel A, Donaldson A. Development of foot-and-mouth disease virus strain characterisation—a review. Trop Ani Heal Prod 1989; 21: 153-166.
  6. Jamal SM, Belsham GJ. Foot-and-mouth disease: past, present and future. Vet Res 2013; 44: 1. Biswal JK, Bisht P, Mohapatra JK, Ranjan R, Sanyal A, Pattnaik B. Application of a recombinant capsid polyprotein (P1) expressed in a prokaryotic system to detect antibodies against foot-and-mouth disease virus serotype O. J Virol Meth 2015; 215: 45-51.
  7. Hoffmann B, Beer M, Reid SM, Mertens P, Oura CA, Van Rijn PA, Slomka MJ, Banks J, Brown IH, Alexander DJ. A review of RT-PCR technologies used in veterinary virology and disease control: sensitive and specific diagnosis of five livestock diseases notifiable to the World Organisation for Animal Health. Vet Microbiol 2009; 139: 1-23. Kitching R. Clinical variation in foot and mouth disease: cattle. Rev Sci Tech OIE 2002; 21: 499-502.
  8. Reid SM, Ferris NP, Hutchings GH, Samuel AR, Knowles NJ. Primary diagnosis of foot-and-mouth disease by reverse transcription polymerase chain reaction. J Virol Met 2000; 89: 167-176.
  9. Reid SM, Ebert K, Bachanek-Bankowska K, Batten C, Sanders A, Wright C, Shaw AE, Ryan ED, Hutchings GH, Ferris NP. Performance of real-time reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus during field outbreaks in the United Kingdom in 2007. J Vet Diag Invest 2009; 21: 321-330.
  10. Reid SM, Ferris NP, Hutchings GH, Zhang Z, Bel