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Journals / Turkish Journal of Veterinary and Animal Sciences / 2015 / Cilt: 39 - Sayı: 5

Heparin-induced in vitro capacitation changes of swamp buffalo spermatozoa

Dibyajyoti TALUKDAR
Kutubuddin AHMED
Sourabh DEORI
Gopal Chandra DAS
Pages
629–633
Publication Date
2015-12-04
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, heparin induces in vitro capacitation changes in swamp buffalo spermatozoa as evidenced by the highest hyperactivation of spermatozoa and live acrosome reaction at 4 h of incubation.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The aim of this study was to evaluate heparin-induced in vitro capacitation-associated changes in spermatozoa of swamp buffalo.
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Key Findings & Data:Therefore, freshly ejaculated and washed spermatozoa of 8 swamp buffalo bulls were capacitated in vitro in TALP medium supplemented with BSA, heparin, and HEPES buffer at a concentration of 6 × 109 spermatozoa/mL at 37 °C for 6 h.
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Conclusion & Impact:In conclusion, heparin induces in vitro capacitation changes in swamp buffalo spermatozoa as evidenced by the highest hyperactivation of spermatozoa and live acrosome reaction at 4 h of incubation.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Swamp buffalo · spermatozoa · capacitation

Abstract

The aim of this study was to evaluate heparin-induced in vitro capacitation-associated changes in spermatozoa of swamp buffalo. Therefore, freshly ejaculated and washed spermatozoa of 8 swamp buffalo bulls were capacitated in vitro in TALP medium supplemented with BSA, heparin, and HEPES buffer at a concentration of 6 × 109 spermatozoa/mL at 37 °C for 6 h. Capacitation status of spermatozoa in terms of the hyperactivated motility, acrosome membrane integrity, total hypoosmotic swelling test (HOST), activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and sperm membrane protein (SMP) and cholesterol content were estimated for each ejaculate at 1-h intervals from 0 to 6 h of incubation. The results revealed that the highest hyperactivation of spermatozoa (74.50 ± 1.78%) and live acrosome reaction (56.92 ± 1.88%) was recorded at 4 h of incubation while the total HOST-reacted spermatozoa and SMP and cholesterol levels decreased significantly (P < 0.01) with increasing period of incubation. The AST and ALT activities increased significantly (P < 0.01) as incubation period increased. In conclusion, heparin induces in vitro capacitation changes in swamp buffalo spermatozoa as evidenced by the highest hyperactivation of spermatozoa and live acrosome reaction at 4 h of incubation.

Özet

The aim of this study was to evaluate heparin-induced in vitro capacitation-associated changes in spermatozoa of swamp buffalo. Therefore, freshly ejaculated and washed spermatozoa of 8 swamp buffalo bulls were capacitated in vitro in TALP medium supplemented with BSA, heparin, and HEPES buffer at a concentration of 6 × 109 spermatozoa/mL at 37 °C for 6 h. Capacitation status of spermatozoa in terms of the hyperactivated motility, acrosome membrane integrity, total hypoosmotic swelling test (HOST), activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and sperm membrane protein (SMP) and cholesterol content were estimated for each ejaculate at 1-h intervals from 0 to 6 h of incubation. The results revealed that the highest hyperactivation of spermatozoa (74.50 ± 1.78%) and live acrosome reaction (56.92 ± 1.88%) was recorded at 4 h of incubation while the total HOST-reacted spermatozoa and SMP and cholesterol levels decreased significantly (P < 0.01) with increasing period of incubation. The AST and ALT activities increased significantly (P < 0.01) as incubation period increased. In conclusion, heparin induces in vitro capacitation changes in swamp buffalo spermatozoa as evidenced by the highest hyperactivation of spermatozoa and live acrosome reaction at 4 h of incubation.

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Cite This Article

TALUKDAR, D., AHMED, K., DEORI, S., DAS, G. C. (2015). Heparin-induced in vitro capacitation changes of swamp buffalo spermatozoa. Turkish Journal of Veterinary and Animal Sciences, Cilt: 39 - Sayı: 5, 629–633. https://ajindex.com/articles/121417

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