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Journals / Turkish Journal of Veterinary and Animal Sciences / 2017 / Cilt: 41 - Sayı: 3

Evidence of foot-and-mouth disease virus excretion in the milk of apparently healthy vaccinated buffaloes in Islamabad, Pakistan

Hafiz İftikhar AHMED
Umer FAROOQ
Aamer Bin ZAHUR
Khalid NAEEM
Asma LATIF
Hamid IRSHAD
Pages
431–434
Publication Date
2017-06-12
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

A high seroprevalence of FMD and isolation of FMDV from the milk of healthy Asian buffaloes may represent a possible role for healthy Asian buffaloes as an FMD reservoir.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of the study was to determine the seroprevalence of foot-and-mouth disease (FMD) and isolation of the FMD virus (FMDV) in the milk of apparently healthy Asian buffaloes.
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Key Findings & Data:A cross-sectional study was conducted in periurban dairy farms (n = 20) on ≥20 animals per farm in the suburbs of Islamabad, Pakistan.
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Conclusion & Impact:A high seroprevalence of FMD and isolation of FMDV from the milk of healthy Asian buffaloes may represent a possible role for healthy Asian buffaloes as an FMD reservoir.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:FMD · seroprevalence · ELISA

Abstract

The aim of the study was to determine the seroprevalence of foot-and-mouth disease (FMD) and isolation of the FMD virus (FMDV) in the milk of apparently healthy Asian buffaloes. A cross-sectional study was conducted in periurban dairy farms (n = 20) on ≥20 animals per farm in the suburbs of Islamabad, Pakistan. Sera samples (n = 200) were collected to monitor FMDV seroconversions. The analysis of serum samples using ELISA indicated a high seroprevalence of FMD (78%; 156/200) in Asian buffaloes. Milk samples were collected from FMDV seropositive animals only, and virus-specific signals were detected in 12.2% (19/156) of the animals by reverse transcriptase real-time PCR (rRT-PCR). Milk samples positive for FMDV using rRT-PCR were inoculated into LF-BK cells for virus isolation. Two FMDV isolates (serotypes O and Asia-1) were recovered from the milk samples. A high seroprevalence of FMD and isolation of FMDV from the milk of healthy Asian buffaloes may represent a possible role for healthy Asian buffaloes as an FMD reservoir.

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Cite This Article

AHMED, H. İ., FAROOQ, U., ZAHUR, A. B., NAEEM, K., LATIF, A., IRSHAD, H. (2017). Evidence of foot-and-mouth disease virus excretion in the milk of apparently healthy vaccinated buffaloes in Islamabad, Pakistan. Turkish Journal of Veterinary and Animal Sciences, Cilt: 41 - Sayı: 3, 431–434. https://ajindex.com/articles/121357

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