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Journals / Journal of Limnology and Freshwater Fisheries Research / 2026 / Cilt: 4 Sayı: 1

Investigating the Effect of Different Growth Media on Biomass Production of Pseudopediastrum boryanum (Turpin) E. Hegewald Isolates

Farklı Besi Ortamlarının Pseudopediastrum boryanum (Turpin) E. Hegewald İzolatlarının Biyokütle Üretimine Etkileri Üzerine Araştırma

Dilek Yalçın Duygu(Gazi University, Faculty of Biology Education, Department of Biology, Ankara)0000-0003-2127-8186
İlkay Açıkgöz Erkaya(Ahi Evran University, Faculty of Architecture and Engineering, Department of Environmental Engineering, Kırşehir, Turkey)0000-0003-1730-4951
Tülay Özer(Ahi Evran University, Kaman High of Applied Science, Food Technology Department, Kırşehir, Turkey)0000-0003-2926-6622
Pages
6–12
Publication Date
2018-04-27
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Growth rates of P. boryanum were found to be 0.6676 d -1 in the Allen and 0.6021 d -1 in the BG-11 medium.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Microalgae Pseudopediastrum boryanum (Turpin) E.
📊
Key Findings & Data:In the present study, we investigated the growth and biomass production of P. boryanum through use of semi-continuous cultures employing two growth media (Allen and BG-11).
💡
Conclusion & Impact:Growth rates of P. boryanum were found to be 0.6676 d -1 in the Allen and 0.6021 d -1 in the BG-11 medium.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Pseudopediastrum boryanum · optik yoğunluk · hücre yoğunluğu

Abstract

Microalgae Pseudopediastrum boryanum (Turpin) E. Hegewald was chosen as a subject for the present research due to its potential uses of wastewater treatment and biodiesel production. In the present study, we investigated the growth and biomass production of P. boryanum through use of semi-continuous cultures employing two growth media (Allen and BG-11). In our previous study, P. boryanum was isolated from different freshwater reservoir through the dilution technique. The isolated P. boryanum strain was inoculated with 270 mL of medium + 30 mL of suspension culture and t he 16:8 light/dark photoperiod was applied. Optical density was recorded by using UV-Visible spectrophotometer at 670 nm, and cell count examination was performed through drop count method. Besides, dry weight and chlorophyll-a concentration of strain were determined. The highest cell density (3.67x10 6 cells/mL), dry weight (0.032 g/mL) and chlorophyll-a (16.39 µgL -1 ) production were observed in the Allen medium. Growth rates of P. boryanum were found to be 0.6676 d -1 in the Allen and 0.6021 d -1 in the BG-11 medium.

Özet

Mikroalg, Pseudopediastrum boryanum (Turpin) E. Hegewald, atık su arıtımı ve biyodizel üretim potansiyelinden dolayı araştırma konusu olarak seçilmiştir. Bu araştırmada, iki besi ortamı (Allen ve BG-11) kullanılarak, kesikli kültür sisteminde P. boryanum 'un büyüme ve biyokütle üretimi araştırılmıştır. Önceki çalışmalarımızda, P. boryanum dilüsyon tekniği kullanılarak, farklı tatlısu birikintilerinden izole edilmiştir. İzole edilen P. boryanum suşu, 270 mL besi ortamı + 30 mL süspansiyon kültür ile aşılanmış ve 16:8 aydınlık/karanlık fotoperiyodu uygulanmıştır. Optik yoğunluk 670 nm'de UV-Visible spektrofotometre kullanılarak tespit edilmiş ve suşların hücre sayımı damla sayım metodu kullanılarak yapılmıştır. Kültürlerin kuru ağırlık ve klorofil-a tayinleri de gerçekleştirilmiştir. Allen ortamında en yüksek hücre yoğunluğu (3,67x10 6 hücre/mL), kuru ağırlık (0,032 g/mL) ve klorofil-a (16,39 μg L -1 ) olarak tespit edilmiştir. P. boryanum ’un büyüme oranları Allen besi ortamında 0,6676 d -1 ve BG-11 ortamında 0,6021 d -1 olarak bulunmuştur.

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Cite This Article

Duygu, D. Y., Erkaya, İ. A., Özer, T. (2018). Investigating the Effect of Different Growth Media on Biomass Production of Pseudopediastrum boryanum (Turpin) E. Hegewald Isolates. Journal of Limnology and Freshwater Fisheries Research, Cilt: 4 Sayı: 1, 6–12. https://doi.org/10.17216/limnofish.348198

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