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Journals / Acta Orthopaedica et Traumatologica Turcica / 2026 / Cilt: 47 Sayı: 2

Effect of low-dose irradiation on structural and mechanical properties of hyaline cartilage-like fibrocartilage

Düşük doz X ışınının hiyalin kıkırdak benzeri fibrokıkırdağın yapısal ve mekanik özellikleri üstündeki etkisi

Tevfik Oncan
Burak Demirag
Cenk Ermutlu
Ulviye Yalcinkaya
Lutfu Ozkan
Pages
127–133
Publication Date
2013-04-26
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

However, structural changes such as chondrocyte clustering were observed.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The aim of this study was to analyze the effect of low-dose irradiation on fibrous cartilage and to obtain a hyaline cartilage-like fibrocartilage (HCLF) with similar structural and mechanical properties to hyaline cartilage.
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Key Findings & Data:Methods: An osteochondral defect was created in 40 knees of 20 rabbits.
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Conclusion & Impact:However, structural changes such as chondrocyte clustering were observed.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Düşük doz X ışını · hiyalin kıkırdak · hiyalin kıkırdak benzeri fibrokıkırdak

Abstract

Objective: The aim of this study was to analyze the effect of low-dose irradiation on fibrous cartilage and to obtain a hyaline cartilage-like fibrocartilage (HCLF) with similar structural and mechanical properties to hyaline cartilage. Methods: An osteochondral defect was created in 40 knees of 20 rabbits. At the 7th postoperative day, a single knee of each rabbit was irradiated with a total dose of 5.0 Gy in 1.0 Gy fractions for 5 days (radiotherapy group), while the other knee was not irradiated (control group). Rabbits were then divided into four groups of 5 rabbits each. The first three groups were sacrificed at the 4th, 8th and the 12th postoperative weeks and cartilage defects were macroscopically and microscopically evaluated. The remaining group of 5 rabbits was sacrificed at the 12th week and biomechanical compression tests were performed on the cartilage defects. Results: There was no significant biomechanical difference between the radiotherapy and the control group (p=0.686). There was no significant macroscopic and microscopic difference between groups (p=0.300). Chondrocyte clustering was observed in the irradiated group. Conclusion: Low-dose irradiation does not affect the mechanical properties of HCLF in vivo. However, structural changes such as chondrocyte clustering were observed.

Özet

Amaç: Bu çalışmanın amacı düşük doz X ışınının fibröz kıkırdak üzerindeki etkilerini araştırmak ve hiyalin kıkırdak ile aynı mekanik ve yapısal özelliklere sahip hiyalin kıkırdak benzeri fibrokıkırdak (HKBF) geliştirmek idi. Çalışma planı: Yirmi tavşanın 40 diz ekleminde osteokondral defekt oluşturuldu. Ameliyat sonrası 7. günde başlanarak her tavşanın tek bir dizine, 5 gün boyunca, günlük 1.0 Gy'lik dozlarla toplam 5.0 Gy X ışını uygulanırken (radyoterapi grubu), diğer dizi uygulama dışı bırakıldı (kontrol grubu). Tavşanlar daha sonra her birinde 5 adet olacak şekilde 4 gruba ayrıldı. İlk üç grup, sırasıyla, 4., 8. ve 12. haftada sakrifiye edildi ve kıkırdak defektleri makroskopik ve mikroskobik olarak incelendi. Kalan 5 tavşanın oluşturduğu grup da 12. haftada sakrifiye edilerek kıkırdak defektlerine biyomekanik sıkıştırma testleri uygulandı. Bulgular: Radyoterapi ve kontrol grupları arasında biyomekanik açıdan anlamlı bir fark saptanmadı (p=0.686). Yine, gruplar arasında makroskopik ve mikroskopik açıdan da anlamlı bir fark yoktu (p=0.300). Işınlanan grupta kondrosit kümelenmesi gözlendi. Çıkarımlar: Düşük doz X ışını HKBF dokusunun mekanik özelliklerini in vivo etkilememekle birlikte, kondrosit kümelenmesi gibi yapısal değişikliklere yol açmaktadır.

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Cite This Article

Oncan, T., Demirag, B., Ermutlu, C., Yalcinkaya, U., Ozkan, L. (2013). Effect of low-dose irradiation on structural and mechanical properties of hyaline cartilage-like fibrocartilage. Acta Orthopaedica et Traumatologica Turcica, Cilt: 47 Sayı: 2, 127–133. https://ajindex.com/articles/1191546

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