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Journals / Kocatepe Veteriner Dergisi / 2026 / Cilt: 19 Sayı: 2

Effect of Ensiling Rice Straw With Sodium Hydroxide and Lactobacillus plantarum Added Corn Silage and Sugar Beet Pulp on Nutritional Value and In Vitro Digestibility

Sodyum Hidroksit ve Lactobacillus plantarum Eklenmiş Mısır Silajı ve Şeker Pancarı Posasıyla Pirinç Samanının Silolanmasının Besin Değeri ve İn Vitro Sindirilebilirlik Üzerine Etkisi

Süleyman Hacısalihoğlu(UŞAK ÜNİVERSİTESİ)0000-0001-9853-3312
İbrahim Sadi Çetingül(AFYON KOCATEPE ÜNİVERSİTESİ)0000-0002-7608-6176
Pages
158–171
Publication Date
2026-06-15
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was observed that rice straw had higher digestibility in the FSBP groups.

📌 3 Key Takeaways

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Objective & Hypothesis:This research was planned to determine the silage making ability and to investigate the effect of adding Corn Silage (CS) and Fresh Sugar Beet Pulp (FSBP) to rice straw on digestibility.
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Key Findings & Data:CS was used in 3 groups and FSBP was used in 3 groups.
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Conclusion & Impact:It was observed that rice straw had higher digestibility in the FSBP groups.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:In Vıtro sindirilebilirlik · Lactobacillus plantarum · Pirinç samanı

Abstract

This research was planned to determine the silage making ability and to investigate the effect of adding Corn Silage (CS) and Fresh Sugar Beet Pulp (FSBP) to rice straw on digestibility. Six main groups were created within the scope of this research. CS was used in 3 groups and FSBP was used in 3 groups. No additives were added to the control group. NaOH was added to a group containing CS and FSBP, and Lactobacillus plantarum (L. plantarum) was added to a group. The samples were left to ferment for 60 days. After fermentation, the groups were analyzed. The samples were then incubated in the Daisyıı incubator for 72 hours. After in vitro digestion, the same analyses were repeated on the samples to determine the in vitro digestion levels. It was observed that the addition of NaOH to the silage samples increased the Dry Matter (DM) digestibility. Crude protein (CP) digestibility was found to be higher in the groups containing FSBP, than in the groups containing CS. In vitro digestion experiments showed decreases in Neutral Detergent Fiber (NDF) and Acid Detergent Fiber (ADF) levels. It was observed that the highest NDF digestion occurred in the groups containing NaOH. In the study, it was observed that NDF and ADF digestion was higher in the FSBP groups. It was observed that rice straw had higher digestibility in the FSBP groups.

Özet

Bu araştırma, silaj olabilme kabiliyetini belirlemek ve pirinç samanına Mısır Silajı (MS) ve Yaş Şeker Pancarı Posası (YŞPP) ilavesinin sindirilebilirliğe etkisini araştırmak amacıyla planlanmıştır. Çalışma kapsamında 6 ana grup oluşturuldu. 3 grupta MS ve 3 grupta YŞPP kullanıldı. Kontrol gruplarına herhangi bir katkı maddesi eklenmedi. MS ve YŞPP içeren bir gruba NaOH, bir gruba ise Lactobacillus plantarum (L. plantarum) eklendi. Numuneler 60 gün süreyle fermente edilmeye bırakıldı. Fermantasyondan sonra gruplar analiz edildi. Daha sonra örnekler Daisyıı inkübatöründe 72 saat süreyle inkübe edildi. İn vitro sindirimden sonra, in vitro sindirim düzeylerini belirlemek amacıyla aynı analizler örnekler üzerinde tekrarlandı. Silaj örneklerine NaOH ilavesinin Kuru Madde (KM) sindirilebilirliğini artırdığı gözlemlendi. Ham protein (HP) sindirilebilirliğinin YŞPP içeren gruplarda MS içeren gruplara göre daha yüksek olduğu bulundu. İn vitro sindirim deneyleri, Nötr Deterjan Lif (NDF) ve Asit Deterjan Lif (ADF) düzeylerinde azalmalar olduğunu gösterdi. En yüksek NDF sindiriminin NaOH içeren gruplarda gerçekleştiği gözlendi. Çalışmada YŞPP gruplarında NDF ve ADF sindiriminin daha yüksek olduğu gözlendi. Pirinç samanının YŞPP gruplarında daha yüksek sindirilebilirliğe sahip olduğu gözlendi.

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Cite This Article

Hacısalihoğlu, S., Çetingül, İ. S. (2026). Effect of Ensiling Rice Straw With Sodium Hydroxide and Lactobacillus plantarum Added Corn Silage and Sugar Beet Pulp on Nutritional Value and In Vitro Digestibility. Kocatepe Veteriner Dergisi, Cilt: 19 Sayı: 2, 158–171. https://doi.org/10.30607/kvj.1811981

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References (34)

  1. Abo-Donia, F. M., Ahmed El-Shora, M., Abd-Elaziz Riad, W., Basuony Elgamal, N., & Abdel-Menaem El-Hamady, W. (2022). Improve the nutritional value and utilization of rice straw via an ensiling process with different sources of energy and nitrogen enrichment. Journal of Applied Animal Research 50(1), 333-341. https://doi.org/10.1080/09712119.2022.2076685
  2. AOAC- Association of Official Analytical Chemists., (1990). Official Methods of Analysis. 15th ed. AOAC, Maryland, USA.
  3. Aresta, M., Dibenedetto, A., & Dumeignil, F., (2012). Biorefinery: from biomass to chemicals and fuels. Ed. Frank Dumeignil, Berlin.
  4. Arısoy, M., (1998). The effect of sodium hydroxide treatment on chemical composition and digestibility of straw. Turkish Journal of Veterinary & Animal Sciences 22(2), 165-170.
  5. Cai, Y. M., (2006). Development of lactic acid bacteria inoculant for whole crop rice silage in Japan. p.85-89. In: International Symposium on Production and Utilization of Whole Crop Rice for Feed. Busan.
  6. Chenost, M., & Kayouli, C., (1997). Roughage Utilization İn Warm Climates. Rome.
  7. De Brabander, D. L., De Boever, J. L., De Smet, A. M., Vanacker, J. M. & Boucqué, C. V., (1999). Evaluation of the physical structure of fodder beets, potatoes, pressed beet pulp, brewers grains, and corn cob silage. Journal of Dairy Science 82(1), 110-121. https://doi.org/10.3168/jds.S0022-0302(99)75215-X
  8. Ding, H., Han, Z., Li, J., Li, X., Dong, Z., Zhao, J., Wang, S., & Shao, T., (2022). Effect of fibrolytic enzymes, cellulolytic fungi and lactic acid bacteria on fermentation characteristics, structural carbohydrate composition and in vitro digestibility of rice straw silage. Fermentation 8(12), 709. https://doi.org/10.3390/fermentation8120709
  9. Flieg, O., (1938). A key for the evaluation of silage samples. p. 112-128. Futterbau und Giirfutterbereitung.
  10. Gao, L.,, Yang, H., Wang, X., Huang, Z., Ishii, M., Igarashi, Y., & Cui, Z., (2008). Rice straw fermentation using lactic acid bacteria. Bioresour Technology 99(8), 2742–2747. https://doi.org/10.1016/j.biortech.2007.07.001
  11. He, L., Chen, N., Lv, H., Wang, C., Zhou, W., Zhang, Q., & Chen, X., (2020). Ensiling characteristics, physicochemical structure and enzymatic hydrolysis of steam-exploded hippophae: Effects of calcium oxide, cellulase and Tween. Bioresour Technology 295, 122268. https://doi.org/10.1016/j.biortech.2019.122268
  12. Hoedtke, S., & Zeyner, A., (2011). Comparative evaluation of laboratory‐scale silages using standard glass jar silages or vacuum‐packed model silages. Journal of the Science of Food and Agriculture 91(5), 841-849. https://doi.org/10.1002/jsfa.4255
  13. Jackson, M. G., (1977). Rice straw as livestock feed. World Animal Review.23(25), 79-81.
  14. Johnson, H. E., Merry, R. J., Davies, D. R., Kell, D. B., Theodorou, M. K. & Griffith, G. W., (2005). Vacuum packing: a model system for laboratory‐scale silage fermentations. Journal of Applied Microbiology 98(1), 106-113. https://doi.org/10.1111/j.1365-2672.2004.02444.x
  15. Karaevli, M. E., & Baytekin, H., (2018). Trakya’da Bazı Kışlık Tek Yıllık Tarla Bitkilerinin Silaj Üretiminde Kullanılma İmkânlarının Araştırılması. ÇOMÜ Ziraat Fakültesi Dergisi 6, 249-254. https://doi.org/10.33202/comuagri.504361
  16. Kim, J. G., Ham, J. S., Li, Y. W., Park, H. S., Huh, C. S., & Park, B. C., (2017). Development of a new lactic acid bacterial inoculant for fresh rice straw silage. Asian-Australasian Journal of Animal Sciences 30(7), 950. https://doi.org/10.5713/ajas.17.0287
  17. Klopfenstein, T. J., Krause, V. E., Jones, M. J., & Woods, W., (1972). Chemical treatment of low quality roughages. Journal of Animal Science 35(2), 418-422. https://doi.org/10.2527/jas1972.352418x
  18. Kuriqi, A., Kuriqi, I., & Poci, E., (2016). Simulink programming for dynamic modeling of activated sludge process: aerator and settler tank case. Fresenius Environmental Bulletin 25(8), 2891.
  19. Li, F., Ke, W., Ding, Z., Bai, J., Zhang, Y., Xu, D., Li, Z., & Guo, X., (2020). Pretreatment of Pennisetum sinese silages with ferulic acid esterase-producing lactic acid bacteria and cellulase at two dry matter contents: fermentation characteristics, carbohydrates composition and enzymatic saccharification. Bioresource Technology 295, 122261. https://doi.org/10.1016/j.biortech.2019.122261
  20. Li, P., Ji, S., Wang, Q., Qin, M., Hou, C., & Shen, Y., (2017). Adding sweet potato vines improve the quality of rice straw silage. Animal Science Journal 88(4), 625-632. https://doi.org/10.1111/asj.12690
  21. Liu, H., Li, X., Hu, J., Zhao, J., Xu, G., Dong, D., Jia, Y., & Shao, T., (2023). Fermentation quality and aerobic stability evaluation of rice straw silage with different ensiling densities. Fermentation 10(1), 20. https://doi.org/10.3390/fermentation10010020
  22. Ma, J., Fan, X., Wu, T., Zhou, J., Huang, H., Qiu, T., Xing, Z., Zhao, Z., Yin, F., & Gan, S., (2023). Lactic acid bacteria and cellulase ımprove the fermentation characteristics, aerobic stability and rumen degradation of mixed silage prepared with amaranth and rice straw. Fermentation 9(9), 853. https://doi.org/10.3390/fermentation9090853
  23. Marlida, Y., Harnentis, H., Nur, Y. S., & Ardani, L. R., (2023). New probiotics (Lactobacillus plantarum and Saccharomyces cerevisiae) supplemented to fermented rice straw-based rations on digestibility and rumen characteristics in vitro. Journal of Advanced Veterinary and Animal Research 10(1), 96-102. https://doi.org/10.5455/javar.2023.j657
  24. Martens, S., Wildner, V., Schulze, J., Richardt, W., Greef, J. M., Zeyner, A., & Steinhöfel, O., (2022). Chemical treatment of straw for ruminant feeding with NaOH or urea–investigative steps via practical application under current European Union conditions. Agricultural and Food Science 31(4), 260-281. https://doi.org/10.23986/afsci.115262
  25. Moss, A. R., Givens, D. I., & Furniss, S., (1993). A comparison of farm-scale methods of application of sodium hydroxide on the nutritive value of a winter wheat straw. Animal Feed Science and Technology 41(3), 199-212. https://doi.org/10.1016/0377-8401(93)90013-A
  26. Müller, C. E., & Johansen, A., (2020). Rebaling of silage and haylage and its effects on forage microbial and chemical composition—A pilot study. Grass and Forage Science 75(2), 216-226. https://doi.org/10.1111/gfs.12480
  27. Prajapati, B. P., & Kango, N., (2023). Rice straw saccharification using cellulolytic cocktail from Aspergillus tubingensis and structure alterations studies of the wall polymer. Biomass Conversion and Biorefinery, 13(2), 961-975. https://doi.org/10.1007/s13399-020-01237-4
  28. Pys, J., Migdal, W., Pucek, T., Živković, B., Fabjan, M., Kosovac, O., & Radović, Č., (2002). Effect of lactic acid bacterial inoculant with enzyme and rolled barley additive on the chemical composition and protein degradation of alfalfa silage. Biotechnology in Animal Husbandry 18(3-4), 33-44. https://doi.org/10.2298/BAH0204033P
  29. Ren, H., Shi, R., Yang, D., Tian, H., Wang, L., Ling, Z., Li, J., Li, L., Sun, Y., & Zheng, Y. (2024). Innovative strategy to enhance bioconversion of sweet sorghum bagasse (SSB) by the combination of bio-fortified ensiling and dilute alkali pretreatment. Industrial Crops and Products, 211, 118208. https://doi.org/10.1016/j.indcrop.2024.118208
  30. Sarwono, K. A., Rohmatussolihat, R., Watman, M., Ratnakomala, S., Astuti, W. D., Fidriyanto, R., Ridwan, R., & Widyastuti, Y., (2022). Characteristics of fresh rice straw silage quality prepared with addition of lactic acid bacteria and crude cellulase. AIMS Agriculture and Food 7(3), 481-499. https://doi.org/10.3934/agrfood.2022030
  31. Schmidt, R. J., Hu, W., Mills, J. A., Kung, Jr. L., (2009). The development of lactic acid bacteria and Lactobacillus buchneri and their effects on the fermentation of alfalfa silage. Journal of Dairy Science 92(10), 5005-5010. https://doi.org/10.3168/jds.2008-1701
  32. Wang, Y., Xia, K., Wang, X. N., Lin, X., Liu, J., Li, Y. J., Liu, X. L., Zhao, W.J., Zhang, Y.G. & Guo, J. H. (2022). Improvement of feed intake, digestibility, plasma metabolites, and lactation performance of dairy cows fed mixed silage of sugar beet pulp and rice straw inoculated with lactic acid bacteria. Journal of dairy science, 105(1), 269-280. https://doi.org/10.3168/jds.2021-20494
  33. Yu, Q., Zhuang, X., Wang, W., Qi, W., Wang, Q., Tan, X., Kong, X., & Yuan, Z., (2016). Hemicellulose and lignin removal to improve the enzymatic digestibility and ethanol production. Biomass and Bioenergy 94, 105-109. https://doi.org/10.1016/j.biombioe.2016.08.005
  34. Zhao, J., Dong, Z., Li, J., Chen, L., Bai, Y., Jia, Y., & Shao, T., (2019). Effects of lactic acid bacteria and molasses on fermentation dynamics, structural and nonstructural carbohydrate composition and in vitro ruminal fermentation of rice straw silage. Asian-Australasian Journal of Animal Sciences 32(6), 783. https://doi.org/10.1016/j.scitotenv.2019.06.236