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Journals / Kocatepe Veteriner Dergisi / 2026 / Cilt: 19 Sayı: 3

Evaluation of the Efficacy of Quercetin Against DNA, Lipid, and Protein Damage in a Diabetic Rat Model

Diyabetik Rat Modelinde Kuersetinin DNA, Lipit ve Protein Hasarına Karşı Etkinliğinin Değerlendirilmesi

Ahmet Büyükben(AFYON KOCATEPE UNIVERSITY, ÇAY VOCATIONAL SCHOOL)0000-0002-5639-7217
Funda Kargın Kıral(ADNAN MENDERES UNIVERSITY)0000-0001-9348-8401
Selim Sekkin(ADNAN MENDERES UNIVERSITY)0000-0002-3795-5375
Pages
353–374
Publication Date
2026-09-15
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, while 70 mg.kg-1 quercetin strongly protects against STZ-induced lipid peroxidation and DNA damage, it fails to prevent the oxidative degradation of plasma proteins.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Diabetes Mellitus (DM) induces oxidative stress, causing severe biomolecular damage.
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Key Findings & Data:Rats received STZ (50 mg.kg-1, i.p.) and were treated with quercetin (20, 50, or 70 mg.kg-1, daily, orally) for 21 days.
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Conclusion & Impact:In conclusion, while 70 mg.kg-1 quercetin strongly protects against STZ-induced lipid peroxidation and DNA damage, it fails to prevent the oxidative degradation of plasma proteins.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Diabetes Mellitus · DNA Hasarı · Komet Analizi

Abstract

Diabetes Mellitus (DM) induces oxidative stress, causing severe biomolecular damage. This study investigated quercetin's protective effects against protein, DNA, and lipid oxidation in streptozotocin (STZ)-induced diabetic rats. Rats received STZ (50 mg.kg-1, i.p.) and were treated with quercetin (20, 50, or 70 mg.kg-1, daily, orally) for 21 days. Malondialdehyde (MDA), advanced oxidation protein products (AOPP), nitrotyrosine (NT), and protein carbonyl content (PCC) were measured, alongside lymphocyte Comet assays. STZ significantly elevated MDA levels compared to controls, but all quercetin treatments effectively reduced MDA concentrations (p<0.05). Conversely, while diabetes increased AOPP, NT, and PCC levels, especially 70 mg.kg-1 quercetin administration paradoxically further elevated NT and PCC levels compared to the untreated STZ group (p<0.05). AOPP levels showed a similar increasing trend at lower doses, but 70 mg.kg-1 quercetin exhibited resistance against AOPP alterations, but there are no significantly differences. Comet assays revealed STZ exacerbated DNA damage, whereas 70 mg.kg-1 quercetin significantly mitigated this damage (p<0.05). In conclusion, while 70 mg.kg-1 quercetin strongly protects against STZ-induced lipid peroxidation and DNA damage, it fails to prevent the oxidative degradation of plasma proteins.

Özet

Diabetes Mellitus (DM), oksidatif stresi indükleyerek şiddetli biyomoleküler hasara neden olur. Bu çalışmada, streptozotosin (STZ) ile indüklenen diyabetik sıçanlarda kuersetinin protein, DNA ve lipit oksidasyonuna karşı koruyucu etkileri araştırılmıştır. Sıçanlara STZ (50 mg.kg-1, i.p.) uygulanmış ve 21 gün boyunca oral yolla kuersetin (20, 50 veya 70 mg.kg-1, gün) tedavisi verilmiştir. Malondialdehit (MDA), ileri oksidasyon protein ürünleri (AOPP), nitrotirozin (NT) ve protein karbonil içeriği (PCC) ölçülmüş; bununla birlikte lenfositlerde Komet analizi gerçekleştirilmiştir. STZ uygulaması, MDA düzeylerini kontrol grubuna göre anlamlı derecede artırırken, tüm kuersetin tedavileri MDA konsantrasyonlarını etkili bir şekilde düşürmüştür (p<0.05). Diğer yandan, diyabet AOPP, NT ve PCC seviyelerini artırırken; özellikle 70 mg.kg-1 kuersetin uygulaması, NT ve PCC düzeylerini tedavi edilmeyen STZ grubuna kıyasla paradoksal bir şekilde daha da yükseltmiştir (p<0.05). AOPP düzeyleri düşük dozlarda benzer bir artış eğilimi göstermiş, 70 mg.kg-1 kuersetin ise AOPP değişimlerine karşı direnç sergilemiştir; ancak istatistiksel olarak anlamlı bir fark bulunmamıştır. Komet analizleri, STZ'nin DNA hasarını şiddetlendirdiğini, 70 mg.kg-1 kuersetinin ise bu hasarı anlamlı ölçüde azalttığını ortaya koymuştur (p<0.05). Sonuç olarak, 70 mg.kg-1 kuersetin STZ kaynaklı lipit peroksidasyonu ve DNA hasarına karşı güçlü bir şekilde koruma sağlarken, plazma proteinlerinin oksidatif yıkımını önlemede yetersiz kalmıştır.

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Cite This Article

Büyükben, A., Kıral, F. K., Sekkin, S. (2026). Evaluation of the Efficacy of Quercetin Against DNA, Lipid, and Protein Damage in a Diabetic Rat Model. Kocatepe Veteriner Dergisi, Cilt: 19 Sayı: 3, 353–374. https://doi.org/10.30607/kvj.1995405

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