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Journals / Kocatepe Veteriner Dergisi / 2026 / Cilt: 19 Sayı: 3

In-vitro antifungal and anticlostridial activity of lyophilized postbiotics from Lactiplantibacillus plantarum, Lacticaseibacillus casei, and Lactobacillus helveticus strains

Lactiplantibacillus plantarum, Lacticaseibacillus casei ve Lactobacillus helveticus Suşlarından Elde Edilen Liyofilize Postbiyotiklerin İn-Vitro Antifungal ve Anticlostridial Etkinliklerinin Belirlenmesi

Müzeyyen Akgöl(FIRAT UNIVERSITY, FACULTY OF VETERINARY MEDICINE)0000-0002-8926-4509
Sinem Bayrak(FIRAT ÜNİVERSİTESİ)0009-0007-4988-3368
Gökhan Kürşad İncili(FIRAT ÜNİVERSİTESİ)0000-0003-1178-3365
Pages
266–275
Publication Date
2026-09-15
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, lyophilized postbiotics derived from Lactobacillus strains demonstrated strong antifungal and anticlostridial activities, with their efficacy increasing in a concentration-dependent manner.

📌 3 Key Takeaways

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Objective & Hypothesis:Molds and Clostridium species are significant microorganisms responsible for food spoilage and are also considered potential threats to public health.
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Key Findings & Data:The pH values of the postbiotics obtained from Lactobacillus spp. ranged from 3.98 to 4.58, while their titratable acidity values ranged from 1.28 - 9.38 g lactic acid/100 mL.
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Conclusion & Impact:In conclusion, lyophilized postbiotics derived from Lactobacillus strains demonstrated strong antifungal and anticlostridial activities, with their efficacy increasing in a concentration-dependent manner.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Antimikrobiyal aktivite · Laktik asit bakterisi · Liyofilizasyon

Abstract

Molds and Clostridium species are significant microorganisms responsible for food spoilage and are also considered potential threats to public health. The aim of this study is to determine the antifungal and anticlostridial activities of lyophilized postbiotics obtained from Lactobacillus spp. (Lb. plantarum, Lb. casei, and Lb. helveticus) and to evaluate their pH and titratable acidity values. In addition, the in vitro antimicrobial effects against strains of Aspergillus parasiticus, Penicillium crysogenum, Clostridium sporogenes, and Clostridium tepidum were investigated (minimum inhibitory (MIC), minimum bactericidal (MBC)/minimum fungicidal concentration (MFC), and zone of inhibition diameters). The pH values of the postbiotics obtained from Lactobacillus spp. ranged from 3.98 to 4.58, while their titratable acidity values ranged from 1.28 - 9.38 g lactic acid/100 mL. The MIC, MBC/MFC, and inhibition zone values of Lactobacillus spp. against the specified mould and Clostridium strains were found to range from 12.5 mg/mL, 50–100 mg/mL, and 17.36–24.25 mm, respectively. In conclusion, lyophilized postbiotics derived from Lactobacillus strains demonstrated strong antifungal and anticlostridial activities, with their efficacy increasing in a concentration-dependent manner.

Özet

Küfler ve Clostridium türleri, gıda bozulmasından sorumlu önemli mikroorganizmalar olup aynı zamanda halk sağlığı için potansiyel bir risk olarak kabul edilmektedir. Bu çalışmanın amacı, Lactobacillus spp. (Lb. plantarum, Lb. casei ve Lb. helveticus) kullanılarak elde edilen liyofilize postbiyotiklerin antifungal ve antiklostridyal aktivitelerini belirlemek ve ayrıca pH ile titre edilebilir asitlik değerlerini değerlendirmektir. Ayrıca Aspergillus parasiticus, Penicillium crysogenum, Clostridium sporogenes ve Clostridium tepidum suşlarına karşı in vitro antimikrobiyal etkileri (minimum inhibisyon (MİK), minimum bakterisidal (MBK)/minimum fungusidal konsantrasyonu (MFK) ve inhibisyon zon çapları) incelenmiştir. Lactiplantibacillus türlerinden elde edilen postbiyotiklerin pH değerleri 3,98 ile 4,58 arasında değişirken, titrasyonla belirlenen asitlik değerlerinin 100 mL ‘de 1,28 ile 9,38 g laktik asit arasında değiştiği bulunmuştur. Lactobacillus suşlarının belirtilen küf ve Clostridium suşlarına karşı MİK, MBK/MFK ve inhibisyon zon değerlerinin sırasıyla 12.5 mg/mL, 50-100 mg/mL ve 17.36-24.25 mm (400 mg/mL) arasında olduğu belirlenmiştir. Sonuç olarak, Lactobacillus suşlarından elde edilen liyofilize postbiyotiklerin güçlü antifungal ve antiklostridiyal aktivite sergilediği ve bu etkinliğin konsantrasyona bağlı olarak arttığı ortaya konulmuştur.

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Cite This Article

Akgöl, M., Bayrak, S., İncili, G. K., *, M. A. (2026). In-vitro antifungal and anticlostridial activity of lyophilized postbiotics from Lactiplantibacillus plantarum, Lacticaseibacillus casei, and Lactobacillus helveticus strains. Kocatepe Veteriner Dergisi, Cilt: 19 Sayı: 3, 266–275. https://doi.org/10.30607/kvj.1937678

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