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Journals / Mediterranean Agricultural Sciences / 1993 / Volume: 5-6 Issue: 1-2

The Use or RFLP (Restriction Fragment Length Polymorphism) in Animal Breeding

RFLP (Restriction Fragment Length Polymorphism) Tekniğinin Hayvan lslahında Kullanılması

Ragıp Tığlı
M. Soner Balcıoğlu
Pages
157–174
Publication Date
1993-12-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In addition, it might be used for breeding programs of economic characters of farm animals.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:RFLP (Restriction Fragment Length Polymorphism) which is very new method of genetic marker appeared with recombinant DNA technology.
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Key Findings & Data:DNA fragments within genome cut with restriction enzymes and separated with electrophoresis followed by hybridizaiton.
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Conclusion & Impact:In addition, it might be used for breeding programs of economic characters of farm animals.
🔬Design:Derleme
📍Scope:Comprehensive Sample
⚙️Variables:Restriction Fragment Length Polymorphism · Hayvan lslahı · genetik marker

Abstract

RFLP (Restriction Fragment Length Polymorphism) which is very new method of genetic marker appeared with recombinant DNA technology. RFLP occur due to point mutations which effect restriction sites of enzymes within effect the lenght of DNA fragment between restriction sites. DNA fragments within genome cut with restriction enzymes and separated with electrophoresis followed by hybridizaiton. RFLP has many advantages over other genetic markers. It is lekely to determine RFLP for any probe-restriction enzyme combination. Also it is possible to observe RFLP from DNA sequences which do not carry information (intron, regulator). Today RFLP is used to determine genetical disases like Thallassemia (Akdeniz Anemisi). In addition, it might be used for breeding programs of economic characters of farm animals.

Özet

Genetik marker olarak çok yeni bir teknik olan RFLP (Restriction Fragmenth Length Polymorphism), rekombinant DNA teknolojisiyle ortaya çıkmış yeni bir kavramdır. RFLP, restriksiyon enzimlerinin DNA da kesim yerlerinin dağılımını etkileyen nokta mutasyonları veya kromozomda kesim bölgeleri arasında DNA fragmenti uzunluğunu etkileyen büyük kromozomal değişimler sonucunda meydana gelir. RFLP, restriksiyon enzimleriyle kesilen ve elektroforetik olarak büyüklüklerine göre ayrılan genoma ait DNA fragmentlerinin daha önce radyoaktif olarak işaretlenmiş sonda(probe)lar kullanılarak autoradyografik olarak gözlenmesidir. RFLP nin diğer genetik markerlere nazaran çok sayıda avantajları vardır. Herhangi bir sonda-enzim kombinasyonu için RFLP’yi tespit etme ihtimali oldukça yüksektir. Ayrıca diğer markerlere nazaran önemli bir avantajı da informasyon taşımayan (intron, regülatör) DNA sıralarında RFLP’ yi gözlemektir. RFLP bugün Thallassemia (Akdeniz Anemisi) gibi kalıtsal hastalıkların teşhisinde kullanılmaktadır. Benzer şekilde çiftlik hayvanlarına ait ekonomik karakterlerle ilişkileri ortaya konulduğunda ıslah programlarında yaygın olarak kullanılabilecektir.

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Cite This Article

Tığlı, R., Balcıoğlu, M. S. (1993). The Use or RFLP (Restriction Fragment Length Polymorphism) in Animal Breeding. Mediterranean Agricultural Sciences, Volume: 5-6 Issue: 1-2, 157–174. https://ajindex.com/articles/1170930

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References (11)

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