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Journals / Karaelmas Fen ve Mühendislik Dergisi / 2025 / Cilt: 15 Sayı: 3

Comprehensive Analysis of Essential Oil Constituents in Lathyrus laxiflorus subsp. laxiflorus via SPME and GC-MS/FID and Their Antimicrobial Properties

Lathyrus laxiflorus subsp. laxiflorus'un Esansiyel Yağ Bileşenlerinin SPME ve GC-MS/FID ile Kapsamlı Analizi ve Antimikrobiyal Özellikleri

Nevin Ulaş Çolak(Karadeniz Teknik Üniversitesi)0000-0003-3200-6688
İrem Kurt0009-0000-9693-2950
Ahmet Yaşar(Karadeniz Teknik Üniversitesi)0000-0002-5487-1536
Kamil Coşkunçelebi(Karadeniz Teknik Üniversitesi)0000-0001-5713-6628
Pages
131–139
Publication Date
2025-11-19
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The antimicrobial test conducted in this study was tested on 6 bacteria and 1 fungus and it was seen that it showed antimicrobial activity.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, the essential oil contents of Lathyrus laxiflorus subsp. laxiflorous plant were investigated comparatively by GC-MS/FID and SPME-GC-MS/FID techniques.
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Key Findings & Data:In addition, the antimicrobial activities of the essential oil obtained by steam distillation were investigated. 27 volatile compounds were detected by GC-MS/FID and 30 volatile compounds were detected by SPME-GC-MS/FID method for the plant L.laxiflorus, and a total of 42 volatile compounds were identified.
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Conclusion & Impact:The antimicrobial test conducted in this study was tested on 6 bacteria and 1 fungus and it was seen that it showed antimicrobial activity.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Antimikrobiyal Aktivite · Esansiyel Yağlar · Lathyrus laxiflorus subsp. Laxiflorus

Abstract

In this study, the essential oil contents of Lathyrus laxiflorus subsp. laxiflorous plant were investigated comparatively by GC-MS/FID and SPME-GC-MS/FID techniques. It is known that some of the essential oils used for medicinal purposes are of plant origin. For this purpose, in this study, the content of the essential oil components obtained by steam distillation method was analyzed by comparing with SPME method and its content was elucidated more comprehensively. In addition, the antimicrobial activities of the essential oil obtained by steam distillation were investigated. 27 volatile compounds were detected by GC-MS/FID and 30 volatile compounds were detected by SPME-GC-MS/FID method for the plant L.laxiflorus, and a total of 42 volatile compounds were identified. It is seen that most of the compounds whose structures were elucidated are of the terpene class. The antimicrobial test conducted in this study was tested on 6 bacteria and 1 fungus and it was seen that it showed antimicrobial activity.

Özet

Bu çalışmada, Lathyrus laxiflorus subsp. laxiflorous bitkisinin uçucu yağ içerikleri GC-MS/FID ve SPME-GC-MS/FID teknikleriyle karşılaştırmalı olarak incelenmiştir. Tıbbi amaçlarla kullanılan uçucu yağların bir kısmının bitkisel kaynaklı olduğu bilinmektedir. Bu amaçla bu çalışmada, buhar damıtma yöntemiyle elde edilen uçucu yağ bileşenlerinin içeriği, SPME yöntemi ile karşılaştırılarak analiz edilmiş ve içeriği daha kapsamlı olarak aydınlatılmıştır. Ayrıca, buhar damıtma yöntemiyle elde edilen uçucu yağın antimikrobiyal aktiviteleri araştırılmıştır. L. laxiflorus bitkisi için GC-MS/FID yöntemiyle 27 adet, SPME-GC-MS/FID yöntemiyle ise 30 adet uçucu bileşik belirlenmiş ve toplam 42 adet uçucu bileşik tanımlanmıştır. Yapıları aydınlatılan bileşiklerin çoğunun terpen sınıfından olduğu görülmektedir. Bu çalışmada yapılan antimikrobiyal test 6 bakteri ve 1 mantar üzerinde denenmiş ve antimikrobiyal aktivite gösterdiği görülmüştür.

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Cite This Article

Çolak, N. U., Kurt, İ., Yaşar, A., Coşkunçelebi, K. (2025). Comprehensive Analysis of Essential Oil Constituents in Lathyrus laxiflorus subsp. laxiflorus via SPME and GC-MS/FID and Their Antimicrobial Properties. Karaelmas Fen ve Mühendislik Dergisi, Cilt: 15 Sayı: 3, 131–139. https://doi.org/10.7212/karaelmasfen.1769460

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