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Journals / Karaelmas Fen ve Mühendislik Dergisi / 2026 / Cilt: 16 Sayı: 2

The Seed and Pollen Micromorphology of the Endemic Sabulina mesogitana subsp. flaccida

Sabulina mesogitana subsp. flaccida Endemiğinin Tohum ve Polen Mikromorfolojisi

Havva Karahan(ZONGULDAK BÜLENT ECEVİT ÜNİVERSİTESİ)0000-0003-0518-6265
Yasin Hazer(ZONGULDAK BÜLENT ECEVİT ÜNİVERSİTESİ)0000-0001-8870-0017
Gamze Erikci(ZONGULDAK BÜLENT ECEVİT ÜNİVERSİTESİ)0009-0008-0188-8941
Tuğçe Koç(ZONGULDAK BÜLENT ECEVİT ÜNİVERSİTESİ)0009-0000-7124-6735
Hatice Çölgeçen(ZONGULDAK BÜLENT ECEVİT ÜNİVERSİTESİ)0000-0001-8246-4279
Pages
1–7
Publication Date
2026-07-24
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Since morphological characteristics provide important data for taxonomic classification, this study presents seed and pollen germination data for the endemic S. mesogitana subsp. flaccida species.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, seed and pollen morphology of the endemic Sabulina mesogitana subsp. flaccida, which naturally occurs in Turkey, was examined by light and electron microscopy.
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Key Findings & Data:The highest germination rate was observed at 34.3% in the medium containing 20 g/L sucrose and pH 5.8.
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Conclusion & Impact:Since morphological characteristics provide important data for taxonomic classification, this study presents seed and pollen germination data for the endemic S. mesogitana subsp. flaccida species.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Endemik tür · in vitro polen çimlendirme · mikromorfoloji

Abstract

In this study, seed and pollen morphology of the endemic Sabulina mesogitana subsp. flaccida, which naturally occurs in Turkey, was examined by light and electron microscopy. The objectives included in vitro germination of seeds, assessment of pollen viability, observation of pollen tube formation, and investigation of seed and pollen micromorphology. Seeds of S. mesogitana subsp. flaccida, collected from the Western Taurus Mountains, including the Küre Mountains National Park, one of the nine forest priority hotspots for conservation in Turkey, along with its buffer zone, were germinated in vitro using Murashige and Skoog (MS) media prepared with different sucrose concentrations and pH. The highest germination rate was observed at 34.3% in the medium containing 20 g/L sucrose and pH 5.8. To determine pollen viability, pollen tube formation, and pollen tube length, different sucrose concentrations were tested; the highest germination rate achieved was 30% in the medium containing 20 g/L sucrose. Additionally, the pollen fertility rate of the species was determined to be approximately 88%. Seed and pollen surface morphology was evaluated by scanning electron microscopy (SEM); seed surfaces on ventral and dorsal sides were found to be papillate and tuberculate, with dark brown to black coloration, while pollen grains exhibited a spherical shape with micro-echinate and micro-perforate ornamentation. Since morphological characteristics provide important data for taxonomic classification, this study presents seed and pollen germination data for the endemic S. mesogitana subsp. flaccida species.

Özet

Bu çalışmada Türkiye’de doğal olarak yetişen Sabulina mesogitana subsp. flaccida endemik türünün tohum ve polen morfolojisi ışık ve elektron mikroskobu ile incelenmiş; tohum ve polenlerinin in vitro koşullarda çimlendirilmesi, polen canlılığının belirlenmesi, polen tüpü oluşumunun gözlemlenmesi ve tohum ile polen mikromorfolojisinin araştırılması amaçlanmıştır. Türkiye’de korunması öncelikli 9 orman sıcak noktadan biri olarak nitelendirilen Küre Dağları Milli Parkı ve onun tampon bölgesini de içine alan Batı Küre Dağlarından toplanan S. mesogitana subsp. flaccida’nın tohumlarının in vitro koşullarda çimlendirilmesi amacıyla farklı sükroz konsantrasyonları ve pH değerlerinde Murashige ve Skoog (MS) besin ortamları hazırlanmıştır. In vitro tohum çimlendirme denemelerinde en yüksek çimlenme oranı 20 g/L sükroz içeren ve pH 5.8 olan ortamda %34.3 olarak belirlenmiştir. Canlı polen sayısı, polen tüpü oluşumu ve polen tüpü uzunluğunu belirlemek amacıyla farklı sükroz konsantrasyonları denenmiş sonuç olarak en yüksek çimlenme oranı %30 ile 20 g/L sükroz içeren ortamda gerçekleşmiştir. Çalışmada ayrıca türün polen fertilitesi ortalama %88 olarak tespit edilmiştir. Tohum ve polen yüzey morfolojisi taramalı elektron mikroskobu (SEM) ile değerlendirilmiş; tohumların ventral ve dorsal yüzeylerinin papilli ve tuberküllü yapıda, renklerinin koyu kahverengi-siyah; polenlerin ise sferoidal formda ve polen ornamentasyonlarının mikroekinat-mikroperforat özellikte olduğu belirlenmiştir. Morfolojik karakterler, bitki taksonomisinde sınıflandırmaya katkı sağlayan önemli veriler sunduğundan, endemik S. mesogitana subsp. flaccida türüne ait tohum ve polen çimlenme verileri bu çalışma ile ortaya konulmuştur.

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Cite This Article

Karahan, H., Hazer, Y., Erikci, G., Koç, T., Çölgeçen, H., *, H. K. (2026). The Seed and Pollen Micromorphology of the Endemic Sabulina mesogitana subsp. flaccida. Karaelmas Fen ve Mühendislik Dergisi, Cilt: 16 Sayı: 2, 1–7. https://doi.org/10.7212/karaelmasfen.1772665

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