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Journals / Turkish Journal of Medical Sciences / 2015 / Cilt: 45 - Sayı: 6

Molecular characteristics of extended spectrum ß-lactamase and carbapenemase genes carried by carbapenem-resistant Enterobacter cloacae in a Chinese university hospital

Lanying CUI
Jingying ZHAO
Juan LU
Pages
1321–1328
Publication Date
2015-12-04
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Effective measures should be taken to control the spread of CREC.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Background/aim: In recent years, the increase in the prevalence of carbapenem-resistant Enterobacter cloacae (CREC) has been significant and has become a global problem.
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Key Findings & Data:Materials and methods: The identification and assessment of the antimicrobial susceptibility of CREC isolates were performed using a VITEK-2 compact system.
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Conclusion & Impact:Effective measures should be taken to control the spread of CREC.
🔬Design:Research Article
📍Scope:2009 Verileri
⚙️Variables:Enterobacter cloacae · carbapenem · extended spectrum ß-lactamase

Abstract

Background/aim: In recent years, the increase in the prevalence of carbapenem-resistant Enterobacter cloacae (CREC) has been significant and has become a global problem. The aim of this study is to characterize CREC extended spectrum β-lactamases (ESBLs) and CREC carbapenemase genes and evaluate the risk factors for CREC infections in a university hospital in China. Materials and methods: The identification and assessment of the antimicrobial susceptibility of CREC isolates were performed using a VITEK-2 compact system. Clonal relatedness was investigated by pulsed-field gel electrophoresis (PFGE). β-Lactamase genes were examined using polymerase chain reaction and nucleotide sequencing. Results: A total of 64 CREC isolates were included in the study. The rate of CREC infections significantly increased between 2009 and 2012. Among the CREC isolates, 39 (60.9%) isolates possessed ESBL genes, and 49 (76.6%) carried carbapenemase genes; blaSHV-12 and blaKPC-2 were the most common genes, respectively. PFGE showed that there were no obvious clonal associations between these CREC isolates, except for 3 isolates. A univariate analysis demonstrated that prior treatment with antibiotics, intravascular catheterization, urinary catheterization, mechanical ventilation, and parenteral nutrition were significant risk factors for CREC infections. Conclusion: The emergence and increase of CREC infections is worrisome. Effective measures should be taken to control the spread of CREC.

Özet

Background/aim: In recent years, the increase in the prevalence of carbapenem-resistant Enterobacter cloacae (CREC) has been significant and has become a global problem. The aim of this study is to characterize CREC extended spectrum β-lactamases (ESBLs) and CREC carbapenemase genes and evaluate the risk factors for CREC infections in a university hospital in China. Materials and methods: The identification and assessment of the antimicrobial susceptibility of CREC isolates were performed using a VITEK-2 compact system. Clonal relatedness was investigated by pulsed-field gel electrophoresis (PFGE). β-Lactamase genes were examined using polymerase chain reaction and nucleotide sequencing. Results: A total of 64 CREC isolates were included in the study. The rate of CREC infections significantly increased between 2009 and 2012. Among the CREC isolates, 39 (60.9%) isolates possessed ESBL genes, and 49 (76.6%) carried carbapenemase genes; blaSHV-12 and blaKPC-2 were the most common genes, respectively. PFGE showed that there were no obvious clonal associations between these CREC isolates, except for 3 isolates. A univariate analysis demonstrated that prior treatment with antibiotics, intravascular catheterization, urinary catheterization, mechanical ventilation, and parenteral nutrition were significant risk factors for CREC infections. Conclusion: The emergence and increase of CREC infections is worrisome. Effective measures should be taken to control the spread of CREC.

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Cite This Article

CUI, L., ZHAO, J., LU, J. (2015). Molecular characteristics of extended spectrum ß-lactamase and carbapenemase genes carried by carbapenem-resistant Enterobacter cloacae in a Chinese university hospital. Turkish Journal of Medical Sciences, Cilt: 45 - Sayı: 6, 1321–1328. https://ajindex.com/articles/113747

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