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Journals / Turkish Journal of Medical Sciences / 2015 / Cilt: 45 - Sayı: 6

miRNA-mediated regulation of heat shock proteins in human ejaculated spermatozoa

Tang WENHAO
Liu DE-FENG
Hong KAI
Zhao LIAN-MING
Mao JIA-MING
Zhuang XIN-JIE
Ma LU-LIN
Jiang HUI
Pages
1285–1291
Publication Date
2015-12-04
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: These results show that specific miRNAs are expressed in human ejaculated spermatozoa.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Background/aim: Heat shock proteins (HSPs) are expressed in human spermatozoa and play a role in sperm function.
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Key Findings & Data:Materials and methods: miRNAs differentially expressed in 8 copies of an oligoasthenozoospermic semen group (OA) were identified by comparison with a normal male semen control group (NC) using microarray technology.
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Conclusion & Impact:Conclusion: These results show that specific miRNAs are expressed in human ejaculated spermatozoa.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Heat-shock proteins · microRNAs · microarray

Abstract

Background/aim: Heat shock proteins (HSPs) are expressed in human spermatozoa and play a role in sperm function. MicroRNAs (miRNAs) regulate gene expression, and the possible involvement of miRNAs in the regulation of HSP gene expression in sperm was investigated in this study. Materials and methods: miRNAs differentially expressed in 8 copies of an oligoasthenozoospermic semen group (OA) were identified by comparison with a normal male semen control group (NC) using microarray technology. Potential targets of HSP proteins among the differentially expressed miRNAs were further investigated. Results: HSP40, HSP60, HSP70, and HSP90 were all found to be expressed in human ejaculated spermatozoa. A total of 32 miRNAs showed significant differences in expression between the OA and NC groups. Ten of these miRNAs encoded potential targets of HSPs. Conclusion: These results show that specific miRNAs are expressed in human ejaculated spermatozoa. These miRNAs appear to be involved in regulating the expression of HSP40, HSP70, and HSP90, and this in turn affects sperm function.

Özet

Background/aim: Heat shock proteins (HSPs) are expressed in human spermatozoa and play a role in sperm function. MicroRNAs (miRNAs) regulate gene expression, and the possible involvement of miRNAs in the regulation of HSP gene expression in sperm was investigated in this study. Materials and methods: miRNAs differentially expressed in 8 copies of an oligoasthenozoospermic semen group (OA) were identified by comparison with a normal male semen control group (NC) using microarray technology. Potential targets of HSP proteins among the differentially expressed miRNAs were further investigated. Results: HSP40, HSP60, HSP70, and HSP90 were all found to be expressed in human ejaculated spermatozoa. A total of 32 miRNAs showed significant differences in expression between the OA and NC groups. Ten of these miRNAs encoded potential targets of HSPs. Conclusion: These results show that specific miRNAs are expressed in human ejaculated spermatozoa. These miRNAs appear to be involved in regulating the expression of HSP40, HSP70, and HSP90, and this in turn affects sperm function.

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Cite This Article

WENHAO, T., DE-FENG, L., KAI, H., LIAN-MING, Z., JIA-MING, M., XIN-JIE, Z., LU-LIN, M., HUI, J. (2015). miRNA-mediated regulation of heat shock proteins in human ejaculated spermatozoa. Turkish Journal of Medical Sciences, Cilt: 45 - Sayı: 6, 1285–1291. https://ajindex.com/articles/113746

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