SavedAdd your journal

Journals / Turkish Journal of Medical Sciences / 2015 / Cilt: 45 - Sayı: 6

PCR investigation of Panton-Valentine leukocidin, enterotoxin, exfoliative toxin, and agr genes in Staphylococcus aureus strains isolated from psoriasis patients

PCR investigation of Panton-Valentine leukocidin, enterotoxin, exfoliative toxin,and agr genes in Staphylococcus aureus strains isolated from psoriasis patients*

Julide Sedef GÖÇMEN
Neriman ŞAHİNER
Mukadder KOÇAK
Zeynep Ceren KARAHAN
Pages
1345–1352
Publication Date
2015-12-04
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Enterotoxin production and the carried accessory gene regulatory locus may be important in the aggravation of psoriasis.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Background/aim: Staphylococcus aureus colonization is a determiner of disease activation in psoriasis patients.
📊
Key Findings & Data:Here we evaluate the presence of genes encoding Panton-Valentine leukocidin (PVL), enterotoxins, TSST-1, exfoliative toxins, and the accessory gene regulatory locus by polymerase chain reaction (PCR) in S. aureus isolates obtained from healthy and diseased skin regions and anterior nares of psoriasis patients and healthy controls.
💡
Conclusion & Impact:Conclusion: Enterotoxin production and the carried accessory gene regulatory locus may be important in the aggravation of psoriasis.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Polymerase chain reaction · Staphylococcus aureus · toxin genes

Abstract

Background/aim: Staphylococcus aureus colonization is a determiner of disease activation in psoriasis patients. Here we evaluate the presence of genes encoding Panton-Valentine leukocidin (PVL), enterotoxins, TSST-1, exfoliative toxins, and the accessory gene regulatory locus by polymerase chain reaction (PCR) in S. aureus isolates obtained from healthy and diseased skin regions and anterior nares of psoriasis patients and healthy controls. Materials and methods: The presence of PVL and toxin genes was investigated, and agr typing was performed by PCR. Results: Eighteen of the isolated strains carried the sei, 1 carried the seb-sec, and 1 carried the seg1397645907enterotoxin gene. Eight of the strains carrying enterotoxin genes were isolated from nasal swabs, 6 from diseased skin swabs, and 4 from healthy skin swabs. None of the strains isolated from the control group carried the agr locus. On the other hand, 11 of the S. aureus strains isolated from the patients carried type 1, 7 carried type 1 + 3, 4 carried type 2, 4 carried type 3, and 1 carried type 1 + 2 agr loci. Conclusion: Enterotoxin production and the carried accessory gene regulatory locus may be important in the aggravation of psoriasis.

Özet

Background/aim: Staphylococcus aureus colonization is a determiner of disease activation in psoriasis patients. Here we evaluate the presence of genes encoding Panton-Valentine leukocidin (PVL), enterotoxins, TSST-1, exfoliative toxins, and the accessory gene regulatory locus by polymerase chain reaction (PCR) in S. aureus isolates obtained from healthy and diseased skin regions and anterior nares of psoriasis patients and healthy controls. Materials and methods: The presence of PVL and toxin genes was investigated, and agr typing was performed by PCR. Results: Eighteen of the isolated strains carried the sei, 1 carried the seb-sec, and 1 carried the seg1397645907enterotoxin gene. Eight of the strains carrying enterotoxin genes were isolated from nasal swabs, 6 from diseased skin swabs, and 4 from healthy skin swabs. None of the strains isolated from the control group carried the agr locus. On the other hand, 11 of the S. aureus strains isolated from the patients carried type 1, 7 carried type 1 + 3, 4 carried type 2, 4 carried type 3, and 1 carried type 1 + 2 agr loci. Conclusion: Enterotoxin production and the carried accessory gene regulatory locus may be important in the aggravation of psoriasis.

🔖

Cite This Article

GÖÇMEN, J. S., ŞAHİNER, N., KOÇAK, M., KARAHAN, Z. C. (2015). PCR investigation of Panton-Valentine leukocidin, enterotoxin, exfoliative toxin, and agr genes in Staphylococcus aureus strains isolated from psoriasis patients. Turkish Journal of Medical Sciences, Cilt: 45 - Sayı: 6, 1345–1352. https://ajindex.com/articles/113692

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (10)

  1. Parisi R, Symmons DP, Griffiths CE, Ashcroft DM. Global epidemiology of psoriasis: a systematic review of incidence and prevalence. J Invest Dermatol 2013; 133: 377–385. 2. Kundakci N, Türsen U, Babiker MO, Gürgey E. The evaluation of the sociodemographic and clinical features of Turkish psoriasis patients. Int J Dermatol 2002; 41: 220–224. 3. Fry L, Baker BS. Triggering psoriasis: the role of infections and medications. Clin Dermatol 2007; 25: 606–615. van de Kerkhof PCM. The evolution of psoriatic lesion. Br J Dermatol 2007; 157: 4–15.
  2. Brooks GF, Carroll KC, Butel JS, Morse SA, Mietzner TA. In: Weitz M, Lebowitz H, editors. Jawetz, Melnick & Adelberg’s Medical Microbiology. 25th ed. New York, NY, USA: McGraw- Hill; 2013. pp 199–206. 6. Lowy FD. Staphylococcus aureus infections. N Engl J Med 1998; 339: 520–532.
  3. Holmes A, Ganner M, McGuane S, Pitt TL, Cookson BD, Kearns AM. Staphylococcus aureus isolates carrying Panton- Valentine leucocidin genes in England and Wales: frequency, characterization, and association with clinical disease. J Clin Microbiol 2005; 43: 2384–2390. 8. Foster TJ, Höök M. Surface protein adhesins of Staphylococcus aureus. Trends Microbiol 1998; 6: 484–488.
  4. Speziale P, Pietrocola G, Rindi S, Provenzano M, Provenza G, Di Poto A, Visai L, Arciola CR. Structural and functional role of Staphylococcus aureus surface components recognizing adhesive matrix molecules of the host. Future Microbiol 2009; 4: 1337–1352.
  5. Marraffini LA, Dedent AC, Schneewind O. Sortases and the art of anchoring proteins to the envelopes of gram-positive bacteria. Microbiol Mol Biol Rev 2006; 70: 192–221.
  6. Maresso AW, Schneewind O. Sortase as a target of anti-infective therapy. Pharmacol Rev 2008; 60: 128–141.
  7. Lina G, Piemont Y, Godail-Gamot F, Bes M, Peter MO, Gauduchon V, Vandenesch F, Etienne J. Involvement of Panton–Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clin Infect Dis 1999; 29: 1128–1132.
  8. Karahan ZC, Dolapçı İ, Tekeli A. Influence of reaction optimization on the results of PCR amplification of Panton- Valentine leukocidin genes among Staphylococcus aureus isolates. Mikrobiyol Bül 2009; 43: 519–528. Foster TJ. Immune evasion by staphylococci. Nat Rev Microbiol 2005; 3: 948–958.
  9. Kaneko J, Kamio Y. Bacterial two-component and hetero- heptameric pore-forming cytolytic toxins: structures pore- forming mechanism organization of the genes. Biosci Biotechnol Biochem 2004; 68: 981–1003.
  10. Menestrina G, Dalla Serra M,