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Journals / Turkish Journal of Medical Sciences / 2018 / Cilt: 48 - Sayı: 5

The use of rapid antigen testing and matrix-assisted laser desorption/ionization-time of flight mass spectrometry in the diagnosis of group A beta-hemolytic streptococci in throat swab samples

Rıdvan DENİZ
Elif AKTAŞ
Ayşe BARIŞ
Banu BAYRAKTAR
Pages
939–944
Publication Date
2018-10-31
DOI
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⚡ TL;DR(Too Long; Didn't Read)

Conclusion: This study shows that both the rapid antigen testing of samples and bacterial identification with MALDI-TOF MS contribute much to the rapid diagnosis of GAS tonsillopharyngitis.

📌 3 Key Takeaways

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Objective & Hypothesis:Background/aim: We aimed to evaluate the efficacy of a rapid antigen test in detecting group A beta-hemolytic streptococci (GAS) in throat samples in comparison with the culture method and to compare the efficiency of matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and traditional methods in identifying GAS in cultures.
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Key Findings & Data:Materials and methods: A total of 3668 throat samples from patients with a prediagnosis of tonsillopharyngitis were assessed by the QuickVue+Strep A antigen test and culture.
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Conclusion & Impact:Conclusion: This study shows that both the rapid antigen testing of samples and bacterial identification with MALDI-TOF MS contribute much to the rapid diagnosis of GAS tonsillopharyngitis.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:MALDI-TOF MS · rapid antigen test · Streptococcus pyogenes

Abstract

Background/aim: We aimed to evaluate the efficacy of a rapid antigen test in detecting group A beta-hemolytic streptococci (GAS) in throat samples in comparison with the culture method and to compare the efficiency of matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and traditional methods in identifying GAS in cultures. Materials and methods: A total of 3668 throat samples from patients with a prediagnosis of tonsillopharyngitis were assessed by the QuickVue+Strep A antigen test and culture. For GAS identification from cultures, bacitracin sensitivity, PYR, and latex agglutination tests and MALDI-TOF MS were used. Results: A total of 567 (15.5%) and 536 (14.6%) of the samples were positive for GAS culture and rapid antigen testing, respectively. The sensitivity and specificity of the rapid antigen test compared to culture was 89.07% and 99%, respectively, while positive and negative predictive values were 94.22% and 98.02%. Traditional methods were in full concordance with MALDI-TOF-MS for all 567 isolates. In all densities of growth in culture, the time to diagnosis with MALDI-TOF MS was significantly lower than with traditional identification tests. Conclusion: This study shows that both the rapid antigen testing of samples and bacterial identification with MALDI-TOF MS contribute much to the rapid diagnosis of GAS tonsillopharyngitis.

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Cite This Article

DENİZ, R., AKTAŞ, E., BARIŞ, A., BAYRAKTAR, B. (2018). The use of rapid antigen testing and matrix-assisted laser desorption/ionization-time of flight mass spectrometry in the diagnosis of group A beta-hemolytic streptococci in throat swab samples. Turkish Journal of Medical Sciences, Cilt: 48 - Sayı: 5, 939–944. https://ajindex.com/articles/113473

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