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Journals / Experimed / 2022 / Volume: 12 Issue: 3

Differential Cytotoxic Activity of a New Cationic Pd(II) Coordination Compound with N4-Tetradentate Hybrid Ligand in Cancer Cell Lines

Merve Erkısa Genel(ISTANBUL UNIVERSITY, İSTANBUL FACULTY OF MEDICINE, İSTANBUL MEDICINE PR.)0000-0002-3127-742X
Selin Selvi(İSTİNYE ÜNİVERSİTESİ, MOLEKÜLER KANSER UYGULAMA VE ARAŞTIRMA MERKEZİ)0000-0003-2762-4780
Ismail Yılmaz(KARABÜK ÜNİVERSİTESİ, FEN FAKÜLTESİ, KİMYA BÖLÜMÜ, KİMYA PR.)0000-0003-1116-0934
Remzi Okan Akar(İSTİNYE ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, TIBBİ BİYOKİMYA ANABİLİM DALI)0000-0001-8687-2034
İlhan Yaylım(İSTANBUL ÜNİVERSİTESİ, AZİZ SANCAR DENEYSEL TIP ARAŞTIRMA ENSTİTÜSÜ, MOLEKÜLER TIP ANABİLİM DALI)0000-0003-2615-0202
Abdurrahman Şengül(ZONGULDAK BÜLENT ECEVİT ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ, KİMYA BÖLÜMÜ, KİMYA PR.)0000-0001-6851-4612
Engin Ulukaya(İSTİNYE ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, TIBBİ BİYOKİMYA ANABİLİM DALI)0000-0003-4875-5472
Journal
Experimed
Pages
—
Publication Date
2022-12-31
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Therefore, complex 1 deserves further attention for proof of concept in animal models.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Successful cancer treatment still requires the discovery of novel compounds that hold promise for chemotherapeutics.
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Key Findings & Data:Materials and Methods: A new cationic palladium(II) coordination compound, [Pd(L)]Cl2·H2O, denoted as Complex 1, where the ligand L is the compound 6,6'-bis(NH-benzimidazol-2-yl)-2,2'-bipyridine), was synthesized and characterized by the attenuated total reflectance (ATR) - fourier-transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (1H NMR), electrospray ionization mass spectrometry (ESI-MS), and carbon-hydrogen-nitrogen (CHN) analyses.
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Conclusion & Impact:Therefore, complex 1 deserves further attention for proof of concept in animal models.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Palladium complexes · N4-donor ligand · benzimidazole

Abstract

Objective: Successful cancer treatment still requires the discovery of novel compounds that hold promise for chemotherapeutics. The objective of this study was to examine the effectiveness of a newly synthesized cationic palladium(II) coordination compound that functions via several pathways to provide an efficient therapeutic option for various cancer cells. Materials and Methods: A new cationic palladium(II) coordination compound, [Pd(L)]Cl2·H2O, denoted as Complex 1, where the ligand L is the compound 6,6'-bis(NH-benzimidazol-2-yl)-2,2'-bipyridine), was synthesized and characterized by the attenuated total reflectance (ATR) - fourier-transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (1H NMR), electrospray ionization mass spectrometry (ESI-MS), and carbon-hydrogen-nitrogen (CHN) analyses. The density functional theory (DFT) calculations show the coordination sphere around the metal center in Complex 1 to be made up of tertiary N atoms of the pyridine (py) and benzimidazole (bim) rings completing the square-planar geometry with significant distortion. The anti-growth/cytotoxic activity of the complex was determined using the sulforhodamine B (SRB) and adenosine triphosphate (ATP) viability assays for 24 and 48 h in vitro. The study evaluates the determinations for annexin V-propidium iodide (PI) positivity, mitochondrial membrane potential loss, Bcl-2 protein inactivation, and deoxyribonucleic acid (DNA) damage to investigate the cell death mode and its partial mechanism. Results: Complex 1 caused cytotoxicity in a dose-dependent manner in all the cell lines used, with IC50 values ranging from 2.6-8.8 μM for 48 h. Among the cancer models, colon and breast cancer cell lines underwent cell death by well-described apoptosis through the intrinsic pathway involving the mitochondria. However, the other cell lines did not show such a cell death modality. This implies that differential cell death modes operate based on the cancer type. Conclusion: For the treatment of breast and colon cancers, the complex 1 appears to be a unique, promising complex. Therefore, complex 1 deserves further attention for proof of concept in animal models.

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Cite This Article

Genel, M. E., Selvi, S., Yılmaz, I., Akar, R. O., Yaylım, İ., Şengül, A., Ulukaya, E. (2022). Differential Cytotoxic Activity of a New Cationic Pd(II) Coordination Compound with N4-Tetradentate Hybrid Ligand in Cancer Cell Lines. Experimed, Volume: 12 Issue: 3. https://doi.org/10.26650/experimed.1188586

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