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Journals / Experimed / 2026 / Volume: 13 Issue: 1

Histopathological and Serum Biomarkers Analyses in MRONJ due to Periodontal Disease in Rats: Comparison of Zoledronic Acid and Denosumab

Ceren Özbek(İSTANBUL ÜNİVERSİTESİ, DİŞ HEKİMLİĞİ FAKÜLTESİ, DİŞ HEKİMLİĞİ (DR))0000-0003-4121-3777
Revan Birke Koca-ünsal(GİRNE ÜNİVERSİTESİ)0000-0003-1540-983X
Merva Soluk Tekkeşin(İSTANBUL ÜNİVERSİTESİ, SAĞLIK BİLİMLERİ ENSTİTÜSÜ, TÜMÖR PATOLOJİSİ (DR))0000-0002-7178-3335
Faruk Çelik(ISTANBUL UNIVERSITY, AZIZ SANCAR INSTITUTE OF EXPERIMENTAL MEDICINE)0000-0003-2433-0277
Hayriye Arzu Ergen(ISTANBUL UNIVERSITY, AZIZ SANCAR INSTITUTE OF EXPERIMENTAL MEDICINE)0000-0001-5736-8453
Ümit Zeybek(ISTANBUL UNIVERSITY, AZIZ SANCAR INSTITUTE OF EXPERIMENTAL MEDICINE)0000-0001-8403-2939
Kıvanç Bektaş Kayhan(İSTANBUL ÜNİVERSİTESİ, DİŞ HEKİMLİĞİ FAKÜLTESİ, DİŞ HEKİMLİĞİ (DR))0000-0001-7149-9230
Meral Ünür(İSTANBUL ÜNİVERSİTESİ, DİŞ HEKİMLİĞİ FAKÜLTESİ, DİŞ HEKİMLİĞİ (DR))0000-0003-4510-1668
Journal
Experimed
Pages
—
Publication Date
2023-05-11
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Since the long-term use of bisphosphonates can cause osteonecrosis in the jaw bones, it should not be overlooked that this can also be caused by chronic inflammatory conditions such as periodontal disease.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: This study aimed to investigate the bisphosphonate and denosumab effects in medication-related osteonecrosis of the jaws (MRONJ) caused by periodontal disease with analyses of serum biomarkers and histopathology.
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Key Findings & Data:The rats were divided into a zoledronic acid group (ZG) (n=12), a denosumab group (DG) (n=12), a saline group (SG) (n=10), and a control group (CG) (n=6).
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Conclusion & Impact:Conclusion: Since the long-term use of bisphosphonates can cause osteonecrosis in the jaw bones, it should not be overlooked that this can also be caused by chronic inflammatory conditions such as periodontal disease.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Bisphosphonates · denosumab · osteonecrosis

Abstract

Objective: This study aimed to investigate the bisphosphonate and denosumab effects in medication-related osteonecrosis of the jaws (MRONJ) caused by periodontal disease with analyses of serum biomarkers and histopathology. Materials and Methods: Forty Copenhagen rats were used in the study. A ligature wire was wrapped around the first molars to induce periodontal disease. The rats were divided into a zoledronic acid group (ZG) (n=12), a denosumab group (DG) (n=12), a saline group (SG) (n=10), and a control group (CG) (n=6). Prostate cancer was induced by injections for ZG, DG, and SG following the ligature application, and injections were repeated on the 14th and 21st days. While periodontal disease was evaluated clinically with gingival edema, swelling and redness, serum osteocalcin, osteopontin, parathormone and receptor activator of nuclear factor-kappa B ligand (RANKL) levels were evaluated using the LUMINEX technique. The Mann-Whitney U test was used for the comparison of parameters between groups (p<0.05). Results: The osteocalcin levels were increased in CG, RANKL levels were decreased in DG, osteopontin levels were increased in ZG, and parathormone levels were increased in both ZG and CG. Conclusion: Since the long-term use of bisphosphonates can cause osteonecrosis in the jaw bones, it should not be overlooked that this can also be caused by chronic inflammatory conditions such as periodontal disease.

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Cite This Article

Özbek, C., Koca-ünsal, R. B., Tekkeşin, M. S., Çelik, F., Ergen, H. A., Zeybek, Ü., Kayhan, K. B., Ünür, M. (2023). Histopathological and Serum Biomarkers Analyses in MRONJ due to Periodontal Disease in Rats: Comparison of Zoledronic Acid and Denosumab. Experimed, Volume: 13 Issue: 1. https://doi.org/10.26650/experimed.1190912

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