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Journals / Experimed / 2026 / Volume: 13 Issue: 2

Gingipain Injection Affects Intestinal OxidantAntioxidant Status and Alkaline Phosphatase in Overfed Zebrafish

Gizem Gündüz(Marmara University, Institute of Health Sciences, Department of Periodontology)0000-0003-1282-1175
Merih Beler(Marmara University, Institute of Health Sciences, Department of Biochemistry)0000-0002-3828-4630
İsmail Ünal(Marmara University, Institute of Health Sciences, Department of Biochemistry)0000-0002-8664-3298
Derya Cansız(Istanbul Medipol University, Faculty of Medicine, Department of Biochemistry)0000-0002-6274-801X
Ebru Emekli Alturfan(Marmara University, Faculty of Dentistry, Department of Biochemistry)0000-0003-2419-8587
Kemal Naci Kose(Marmara University, Faculty of Dentistry, Department of Periodontology)0000-0002-0423-8011
Journal
Experimed
Pages
—
Publication Date
2023-09-18
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Our findings demonstrate that gingipain injection alters the ALP activity and intestinal oxidant-antioxidant status in overfed zebrafish.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Porphyromonas gingivalis (P. gingivalis), a major periodontopathogen, is associated with overfeeding disorders, including metabolic syndrome.
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Key Findings & Data:Materials and Methods: Four groups of healthy adult zebrafish were placed in random tanks as C: Control (n=15); GP: Gingipain (n=15); OF: Overfeeding (n=15); and OF+GP: Overfeeding+Gingipain (n=15) groups.
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Conclusion & Impact:Conclusion: Our findings demonstrate that gingipain injection alters the ALP activity and intestinal oxidant-antioxidant status in overfed zebrafish.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Gingipain · zebrafish · oxidative stress

Abstract

Objective: Porphyromonas gingivalis (P. gingivalis), a major periodontopathogen, is associated with overfeeding disorders, including metabolic syndrome. Gingipains are one of the most powerful endotoxins of P. gingivalis. Our aim was to reveal the effects of gingipain injections on the intestinal oxidant-antioxidant status and alkaline phosphatase (ALP) activity in overfed zebrafish. Materials and Methods: Four groups of healthy adult zebrafish were placed in random tanks as C: Control (n=15); GP: Gingipain (n=15); OF: Overfeeding (n=15); and OF+GP: Overfeeding+Gingipain (n=15) groups. At the end of the experiment, levels of intestinal lipid peroxidation (LPO) and ALP, glutathione S-transferase (GST), and catalase (CAT) activities were evaluated. Results: Intestinal LPO was significantly lower in the GP and OF groups compared to C. Gingipain injection in OF (OF+GP) significantly elevated LPO when compared to C, GP, and OF groups. ALP activities decreased significantly in the GP, OF, and OF+GP compared to C. GST activities increased significantly in the GP when compared to C. Decreased GST activities were observed in the OF and OF+GP. This decrease was less in OF+GP. CAT activities significantly decreased in all groups when compared to C. Conclusion: Our findings demonstrate that gingipain injection alters the ALP activity and intestinal oxidant-antioxidant status in overfed zebrafish.

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Cite This Article

Gündüz, G., Beler, M., Ünal, İ., Cansız, D., Alturfan, E. E., Kose, K. N. (2023). Gingipain Injection Affects Intestinal OxidantAntioxidant Status and Alkaline Phosphatase in Overfed Zebrafish. Experimed, Volume: 13 Issue: 2. https://doi.org/10.26650/experimed.1268503

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