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Journals / Experimed / 2026 / Volume: 14 Issue: 2

Gene Expression Profile as a Precursor of Inflammation in Mouse Models: BFMI860 and C57BL/6NCrl

Ayça Doğan(ALTINBAS UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES (MEDICINE), DEPARTMENT OF PHYSIOLOGY (MEDICINE))0000-0002-6020-8327
Gudrun A. Brockmann(Humboldt University of Berlin, Albrecht Daniel Thaer-Institute for Agricultural and Horticultural Sciences, Animal Breeding Biology and Molecular Genetics)0000-0002-4387-2947
Journal
Experimed
Pages
—
Publication Date
2024-08-26
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The BFMI strain is a valuable and promising model for clarifying the mechanisms underlying obesity and the activation of inflammation in adipose tissue.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: We aimed to investigate the differences in the immune response to body fat content between the genetically mutant obese BFMI860 (BFMI) mouse strain and the lean C57BL/6Ncrl (B6) mouse strain as a control and the effects of obesity on gene expression on inflammation-related pathways in epididymal adipose tissue.
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Key Findings & Data:At the age of 10 weeks, serum and epididymal adipose tissue samples were collected for cytokine and gene expression analyses.
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Conclusion & Impact:The BFMI strain is a valuable and promising model for clarifying the mechanisms underlying obesity and the activation of inflammation in adipose tissue.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Obesity · high-fat diet · adipocytokine signaling pathway

Abstract

Objective: We aimed to investigate the differences in the immune response to body fat content between the genetically mutant obese BFMI860 (BFMI) mouse strain and the lean C57BL/6Ncrl (B6) mouse strain as a control and the effects of obesity on gene expression on inflammation-related pathways in epididymal adipose tissue. Materials and Methods: Six males from each strain were maintained on a standard maintenance diet (SMD) or a high-fat diet (HFD). At the age of 10 weeks, serum and epididymal adipose tissue samples were collected for cytokine and gene expression analyses. RNA samples from epididymal adipose tissue were hybridized using the microarray technique to study the quantitative transcript amounts of genes. Results: Pathway analysis of gene expression data revealed no considerable development of inflammatory state in BFMI and B6 on SMD. Both strains responded to HFD distinctly; the inflammatory state was more prominent in the obese BFMI group than in the lean B6 group. Several genes, such as Adipoq, NFkbia, Plaur, F2r, C3ar1, and Nfatc4 in pathways involved in the immune system have been found to be differentially regulated in BFMI mice. Under the condition of obesity in BFMI mice, the induction of inflammation-related pathways indicates an increased risk of insulin resistance, atherosclerosis, and cardiovascular disease. Conclusion: This study identified distinct expression patterns of genes involved in inflammatory pathways, particularly those associated with the adipocytokine signaling pathway and complement and coagulation cascades, in the epididymal adipose tissue of BFMI and B6 mice. The BFMI strain is a valuable and promising model for clarifying the mechanisms underlying obesity and the activation of inflammation in adipose tissue.

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Cite This Article

Doğan, A., Brockmann, G. A. (2024). Gene Expression Profile as a Precursor of Inflammation in Mouse Models: BFMI860 and C57BL/6NCrl. Experimed, Volume: 14 Issue: 2. https://doi.org/10.26650/experimed.1384602

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