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Journals / Experimed / 2024 / Volume: 14 Issue: 3

Evaluation of the Molecular Effects of the Anticancer Adjuvant Valproic Acid on HEK293T Cells

Hazal Banu Olgun Celebioglu(Graduate School of Health Sciences, Istanbul University)0000-0001-8716-5040
Yeliz Ekici(Graduate School of Health Sciences, Istanbul University)0000-0002-6750-6378
Abdullah Yılmaz(Department of Immunology, Aziz Sancar Institute of Experimental Medicine, Istanbul University)0000-0002-3003-9956
Feyza Nur Tuncer(Department of Genetics, Aziz Sancar Institute of Experimental Medicine)0000-0001-8233-1839
Journal
Experimed
Pages
—
Publication Date
2024-12-19
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Preliminary results indicated no proliferative effect of VPA treatment.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Valproic acid (VPA) can induce apoptosis while inhibiting proliferation in various cancer cells.
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Key Findings & Data:This study adopted a novel strategy for investigating the molecular effects of VPA on non-cancerous cells.
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Conclusion & Impact:Conclusion: Preliminary results indicated no proliferative effect of VPA treatment.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:HEK293T · VPA · apoptosis

Abstract

Objective: Valproic acid (VPA) can induce apoptosis while inhibiting proliferation in various cancer cells. This study adopted a novel strategy for investigating the molecular effects of VPA on non-cancerous cells. Building upon our previous work, which examined the effects of VPA in PANC-1 cells, we now turn our attention to HEK293T cells to determine the effect of VPA in non-cancerous cells. Materials and Methods: HEK293T cells were treated with 2.5 and 5-mM VPA. Flow cytometry measurements were performed on the 24th, 48th, 72nd, and 96th h. Assays for apoptosis and proliferation were conducted using annexin V/ propidium iodide (PI) staining and carboxyfluorescein-succinimidyl ester (CFSE), respectively. Results: No statistical significance was detected between the two different doses of VPA-treated cells and the controls at any time point. While early apoptosis values remained stable until 72 h in all groups, an increase was monitored in cells subjected to VPA for 96 h. Conclusion: Preliminary results indicated no proliferative effect of VPA treatment. However, it may induce apoptosis in long-term incubations. Nevertheless, additional doses of VPA at increased concentrations should be administered to explore cytotoxic levels and their impact on proliferation and apoptosis.

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Cite This Article

Celebioglu, H. B. O., Ekici, Y., Yılmaz, A., Tuncer, F. N. (2024). Evaluation of the Molecular Effects of the Anticancer Adjuvant Valproic Acid on HEK293T Cells. Experimed, Volume: 14 Issue: 3. https://doi.org/10.26650/experimed.1500633

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