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Journals / Experimed / 2026 / Volume: 16 Issue: 2

The Effect of Verbascoside on DNA Damage and Oxidative Stress in 3T3-L1 Adipocytes

Melek Tunç Ata(PAMUKKALE UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF PHYSIOLOGY)0000-0002-0384-2356
Gizem Akan(PAMUKKALE UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF PHYSIOLOGY)0009-0000-3336-102X
Şefike Sude Güvenç(PAMUKKALE UNIVERSITY, SCHOOL OF MEDICINE)0009-0001-8229-0747
Deniz Çelebi(PAMUKKALE UNIVERSITY, SCHOOL OF MEDICINE)0009-0001-0542-1627
Selen Şafak(PAMUKKALE UNIVERSITY, SCHOOL OF MEDICINE)0009-0007-3619-896X
Zeynep Azra Ünal(PAMUKKALE UNIVERSITY, SCHOOL OF MEDICINE)0009-0001-7726-3146
Safa Korkmaz(PAMUKKALE UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF PHYSIOLOGY)0009-0000-2480-5514
Emine Kılıç Toprak(PAMUKKALE UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF PHYSIOLOGY)0000-0002-8795-0185
Journal
Experimed
Pages
—
Publication Date
2026-08-28
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These findings demonstrate that at appropriate doses, verbascoside is a candidate for preventing metabolic disorders, though further molecular studies are needed.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: Obesity involves excessive lipid accumulation in adipose tissue, triggering oxidative stress and DNA damage.
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Key Findings & Data:This study evaluated the effects of verbascoside doses (50 μM: V50 and 100 μM: V100) on DNA damage and oxidative stress in mature adipocytes.
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Conclusion & Impact:These findings demonstrate that at appropriate doses, verbascoside is a candidate for preventing metabolic disorders, though further molecular studies are needed.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Adipocyte · DNA damage · Obesity

Abstract

Objective: Obesity involves excessive lipid accumulation in adipose tissue, triggering oxidative stress and DNA damage. This study evaluated the effects of verbascoside doses (50 μM: V50 and 100 μM: V100) on DNA damage and oxidative stress in mature adipocytes. Materials and Methods: 3T3-L1 cells were differentiated into mature adipocytes and treated with V50 or V100. Oil Red O staining quantified the lipid accumulation. DNA damage was evaluated using the Comet assay. We assessed oxidative stress by calculating the oxidative stress index (OSI) based on total oxidant status (TOS) and total antioxidant status (TAS) measurements. Results: V100 reduced lipid accumulation compared with the control group. Furthermore, while V50 increased DNA damage (tail intensity and moment), V100 significantly lowered it compared to V50. Additionally, V100 improved oxidative stress markers (increased TAS, decreased TOS, and OSI), whereas V50 had no significant effect on these parameters. Conclusion: Verbascoside showed dose-dependent effects on mature adipocytes. While V50 increased DNA damage independently of oxidative stress, V100 improved oxidative status and reduced DNA damage. These findings demonstrate that at appropriate doses, verbascoside is a candidate for preventing metabolic disorders, though further molecular studies are needed.

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Cite This Article

Ata, M. T., Akan, G., Güvenç, Ş. S., Çelebi, D., Şafak, S., Ünal, Z. A., Korkmaz, S., Toprak, E. K. (2026). The Effect of Verbascoside on DNA Damage and Oxidative Stress in 3T3-L1 Adipocytes. Experimed, Volume: 16 Issue: 2. https://doi.org/10.26650/experimed.1892531

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References (27)

  1. World Health Organization. Obesity and overweight. 2025. Available from: https://www.who.int/news-room/fact-sheets/detail/obesity-and-overweight. google scholar
  2. Liu F, He J, Wang H, Zhu D, Bi Y. Adipose morphology: a critical factor in regulation of human metabolic diseases and adipose tissue dysfunction. Obes Surg 2020 30(12): 5086-100. google scholar
  3. Hotamisligil GS. Inflammation and metabolic disorders. Nature 2006 444(7121): 860–7. google scholar
  4. Yu J, Qiu J, Zhang Z, Cui X, Guo W, Sheng M, et al. Redox biology in adipose tissue physiology and obesity. Adv Biol (Weinh) 2023 7(9): e2200234. google scholar
  5. Włodarczyk M, Nowicka G. Obesity, DNA damage, and development of obesity-related diseases. Int J Mol Sci 2019 20(5): 1146. google scholar
  6. Voronkova YS, Voronkova OS, Gorban VA, Holoborodko KK. Oxidative stress, reactive oxygen species, antioxidants: a review. Ecol Noospherol 2018 29(1): 52-5. google scholar
  7. Escobar Marcillo DI, Guglielmi V, Privitera GF, Signore M, Simonelli V, Manganello F, et al. The dual nature of DNA damage response in obesity and bariatric surgery-induced weight loss. Cell Death Dis 2024 15(9): 664. google scholar
  8. Yahfoufi N, Alsadi N, Jambi M, Matar C. The immunomodulatory and anti-inflammatory role of polyphenols. Nutrients 2018 10(11): 1618. google scholar
  9. Boccellino M, D'Angelo S. Anti-obesity effects of polyphenol intake: current status and future possibilities. Int J Mol Sci 2020 21(16): 5642. google scholar
  10. Mohammed RA, Kamel AS, Hindam MO, El-Dessouki AM, Hamouda HA, Ramadan NM, et al. Acteoside as a multifunctional natural glycoside: therapeutic potential across various diseases. Inflammopharmacology 2025. https://doi.org/10.1007/s10787-025-01811-0. google scholar
  11. Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem 2004 37(2): 112–9. google scholar
  12. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005 38(12): 1103–11. google scholar
  13. Prieto-Hontoria PL, Pérez-Matute P, Fernández-Galilea M, Martínez JA, Moreno-Aliaga MJ. Lipoic acid inhibits leptin secretion and Sp1 activity in adipocytes. Mol Nutr Food Res 2011 55(7): 1059-69. google scholar
  14. Dragunow M, Cameron R, Narayan P, O'Carroll S. Image-based high-throughput quantification of cellular fat accumulation. J Biomol Screen 2007 12(7): 999-1005. google scholar
  15. Olivares-Vicente M, Sánchez-Marzo N, Encinar JA, de la Luz Cádiz-Gurrea M, Lozano-Sánchez J, Segura-Carretero A, et al. The potential synergistic modulation of AMPK by Lippia citriodora compounds as a target in metabolic disorders. Nutrients 2019 11(12): 2961. google scholar
  16. Özbek NE, Kılıç Toprak E, Çört A, Altıntaş F, Tokgün O, Küçükatay V. Verbascoside promotes lipolysis in 3T3-L1 hypertrophic cells through alteration in the expression of lipolysis-related genes. Pam Tıp Derg 2026 19(1): 13-26. google scholar
  17. Cevik M, Caker S, Deliorman G, Cagatay P, Gunduz MK, Susleyici B. The effects of glipizide on DNA damage and nuclear transport in differentiated 3T3-L1 adipocytes. Mol Biol Rep 2022 49(2): 1151-9. google scholar
  18. León-González AJ, Auger C, Schini-Kerth VB. Pro-oxidant activity of polyphenols and its implication on cancer chemoprevention and chemotherapy. Biochem Pharmacol 2015 98(3): 371-80. google scholar
  19. Santoro A, Bianco G, Picerno P, Aquino RP, Autore G, Marzocco S, et al. Verminoside- and verbascoside-induced genotoxicity on human lymphocytes: involvement of PARP-1 and p53 proteins. Toxicol Lett 2008 178(2): 71-6. google scholar
  20. Kaewdaungdee S, Sham ZW, Ho HX, Lee SY, Tanee T, Benoit YD, et al. Barleria extracts containing barlerin and verbascoside boost immunity and regulate CYP450 gene in prostate cancer. Sci Rep 2025 15(1): 21942. google scholar
  21. Marčetić M, Bufan B, Drobac M, Antić Stanković J, Arsenović Ranin N, Milenković MT, et al. Multifaceted biological properties of verbascoside/acteoside: antimicrobial, cytotoxic, anti-inflammatory, and immunomodulatory effects. Antibiotics (Basel) 2025 14(7): 697. google scholar
  22. Collins AR. The comet assay for DNA damage and repair: principles, applications, and limitations. Mol Biotechnol 2004 26(3): 249-61. google scholar
  23. Calabrese EJ, Blain R. The occurrence of hormetic dose responses in the toxicological literature, the hormesis database: an overview. Toxicol Appl Pharmacol 2005 202(3): 289-301. google scholar
  24. Calabrese EJ, Kozumbo WJ. The hormetic dose-response mechanism: Nrf2 activation. Pharmacol Res 2021 167: 105526. google scholar
  25. Li N, Hao L, Li S, Deng J, Yu F, Zhang J, et al. The NRF-2/HO-1 signaling pathway: A promising therapeutic target for metabolic dysfunction-associated steatotic liver disease. J Inflamm Res 2024 17: 8061-83. google scholar
  26. Herranz-López M, Barrajón-Catalán E, Segura-Carretero A, Menéndez JA, Joven J, Micol V. Lemon verbena (Lippia citriodora) polyphenols alleviate obesity-related disturbances in hypertrophic adipocytes through AMPK-dependent mechanisms. Phytomedicine 2015 22(6): 605-14. google scholar
  27. Hao DD, Zhang L, Liu SL, Sun ZJ, Si RG, Zhang FN, et al. Acteoside inhibits hepatic lipid accumulation and oxidative stress in type 2 diabetic mice via the Nrf2/HO-1 Pathway. J Toxicol Environ Health A 2025 88(15): 616-25. google scholar