SavedAdd your journal

Journals / Trakya University Journal of Natural Sciences / 2022 / Volume: 23 Issue: 2

EVALUATION OF THE ANTIBIOFILM EFFECT OF FLUCONAZOLE LOADED PLGA NANOPARTICLES PREPARED USING RHAMNOLIPID ON Candida albicans

Ayşe Üstün(ERZURUM TECHNICAL UNIVERSITY)0000-0002-4723-052X
Serkan Örtucu(ERZURUM TECHNICAL UNIVERSITY)0000-0002-3180-0444
Pages
—
Publication Date
2022-10-15
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These results show that the PVA-R-FLZ-PLGA drug delivery system is a new therapeutic approach that can be used in infections caused by C. albicans.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, fluconazole (FLZ) loaded poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) were prepared with two different formulations consisting of polyvinyl alcohol (PVA) and PVA-rhamnolipid (R) in order to improve antibiofilm activity against Candida albicans ATCC 90028.
📊
Key Findings & Data:Drug loading capacity and encapsulation efficiency percentages were measured by spectrophotometric method.
💡
Conclusion & Impact:These results show that the PVA-R-FLZ-PLGA drug delivery system is a new therapeutic approach that can be used in infections caused by C. albicans.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Drug delivery · PLGA nanoparticles · Sustained release

Abstract

In this study, fluconazole (FLZ) loaded poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) were prepared with two different formulations consisting of polyvinyl alcohol (PVA) and PVA-rhamnolipid (R) in order to improve antibiofilm activity against Candida albicans ATCC 90028. The encapsulation efficiency, drug loading capacity, in-vitro release, characterization and antibiofilm activity of these formulations were compared. Characterization of NPs were analyzed by scanning electron microscopy (SEM) and Zetasizer. Drug loading capacity and encapsulation efficiency percentages were measured by spectrophotometric method. PLGA-NPs were spherical in shape with mean sizes of ~300 nm and surface charge of FLZ loaded PVA and PVA-R-PLGA NPs -25,9±1.99, -48,1±2.46, respectively. Sustained release of FLZ (≥60% after 6 h) were obtained in PVA-R PLGA-NPs. The encapsulation efficiency percentages of PVA-FLZ-PLGA and PVA-R-FLZ-PLGA were 50% and 85%, respectively. Antibiofilm inhibition percentages are 55% and 63%, respectively. These results show that the PVA-R-FLZ-PLGA drug delivery system is a new therapeutic approach that can be used in infections caused by C. albicans.

Özet

Bu çalışmada, flukonazol (FLZ) yüklü poli(laktik-ko-glikolik asit) (PLGA) nanopartikülleri (NP'ler), Candida albicans ATCC 90028'e karşı antibiyofilm aktiviteyi artırmak için polivinil alkol (PVA) ve PVA-rhamnolipid (R)'den oluşan iki farklı formülasyon ile hazırlandı. Bu formülasyonların enkapsülasyon etkinliği, ilaç yükleme kapasitesi, in vitro salınım, karakterizasyonu ve antibiyofilm aktivitesi karşılaştırıldı. NP'lerin karakterizasyonu, taramalı elektron mikroskobu (SEM) ve Zetasizer ile analiz edildi. İlaç yükleme kapasitesi ve enkapsülasyon etkinliği yüzdeleri, spektrofotometrik yöntemle ölçüldü. PLGA-NP'ler, ortalama büyüklükleri ~300 nm, küresel şekilli ve FLZ yüklü PVA ve PVA-R-PLGA-NP'lerin yüzey yükü sırasıyla -25,9±1.99, -48,1±2.46'dır. PVA-R-PLGA-NP'lerde sürekli FLZ salınımı (6 saat sonra >%60) elde edildi. PVA-FLZ-PLGA ve PVA-R-FLZ-PLGA'nın enkapsülasyon etkinliği yüzdeleri sırasıyla %50 ve %85'tir. Antibiyofilm inhibisyon yüzdeleri sırasıyla %55 ve %63'tür. Bu sonuçlar, PVA-R-FLZ-PLGA ilaç taşıma sisteminin C.albicans'ın neden olduğu enfeksiyonlarda kullanılabilecek yeni bir tedavi yaklaşımı olduğunu göstermektedir.

🔖

Cite This Article

Üstün, A., Örtucu, S. (2022). EVALUATION OF THE ANTIBIOFILM EFFECT OF FLUCONAZOLE LOADED PLGA NANOPARTICLES PREPARED USING RHAMNOLIPID ON Candida albicans. Trakya University Journal of Natural Sciences, Volume: 23 Issue: 2. https://doi.org/10.23902/trkjnat.1103047

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (26)

  1. Ahmed, R., Tariq, M., Ahmad, I.S., Fouly, H., Fakhar-i-Abbas, Hasan, A. & Kushad, M. 2019. Poly(lactic-co-glycolic acid) Nanoparticles Loaded with Callistemon citrinus Phenolics Exhibited Anticancer Properties against Three Breast Cancer Cell Lines. Journal of Food Quality, e2638481. https://doi.org/10.1155/2019/2638481
  2. Alhowyan, A.A., Altamimi, M.A., Kalam, M.A., Khan, A.A., Badran, M., Binkhathlan, Z., Alkholief, M. & Alshamsan, A. 2019. Antifungal efficacy of Itraconazole loaded PLGA-nanoparticles stabilized by vitamin-E TPGS: In vitro and ex vivo studies. Journal of Microbiological Methods, 161: 87-95. https://doi.org/10.1016/j.mimet.2019.01.020
  3. de Alteriis, V., Maselli, A., Falanga, S., Galdiero, F.M., Di Lella, R., Gesuele, M., Guida. & E. Galdiero. 2018. Efficiency of gold nanoparticles coated with the antimicrobial peptide indolicidin against biofilm formation and development of Candida spp. clinical isolates, Infect Drug Resist. 11: 915-925. https://doi.org/10.2147/IDR.S164262.
  4. Cheow, W.S. & Hadinoto, K. 2012. Lipid-polymer hybrid nanoparticles with rhamnolipid-triggered release capabilities as anti-biofilm drug delivery vehicles. Particuology, 10(3): 327-333. https://doi.org/10.1016/j.partic.2011.08.007
  5. de Barros, P. P., Rossoni, R. D., de Souza, C. M., Scorzoni, L., Fenley, J. D. C., & Junqueira, J. C. 2020. Candida biofilms: an update on developmental mechanisms and therapeutic challenges. Mycopathologia, 185(3): 415-424.
  6. Gómez-Sequeda, N., Torres, R. & Ortiz, C. 2017. Synthesis, characterization, and in vitro activity against Candida spp. of fluconazole encapsulated on cationic and conventional nanoparticles of poly(lactic-co-glycolic acid). Nanotechnol Science and Applications, 10; 95-104. https://doi.org/10.2147/NSA.S96018
  7. Han, C., Romero, N., Fischer, S., Dookran, J., Berger, A. & Doiron, A.L. 2017. Recent developments in the use of nanoparticles for treatment of biofilms. Nanotechnology Reviews, 6(5): 383-404. https://doi.org/10.1515/ntrev-2016-0054
  8. Hazen, K.C. & Howell, S.A. 2003. Candida, Cryptococcus, and other yeasts of medical importance. Manual of Clinical Microbiology, 2: 1693-1711.
  9. Kalam, M.A., Alshehri, S., Alshamsan, A., Haque, A. & Shakeel, F. 2017. Solid liquid equilibrium of an antifungal drug itraconazole in different neat solvents: Determination and correlation. Journal of Molecular Liquids, 234: 81--87. https://doi.org/10.1016/j.molliq.2017.03.054
  10. Lee, Y., Lee, D., Park, E., Jang, S., Cheon, S.Y., Han, S. & Koo, H. 2021. Rhamnolipid-coated W/O/W double emulsion nanoparticles for efficient delivery of doxorubicin/erlotinib and combination chemotherapy. Journal of Nanobiotechnology 19(1): 411. https://doi.org/10.1186/s12951-021-01160-4
  11. Lü, J.-M., Wang, X., Marin-Muller, C., Wang, H., Lin, P.H., Yao, Q. & Chen, C. 2009. Current advances in research and clinical applications of PLGA-based nanotechnology. Expert Review of Molecular Diagnostics, 9(4): 325-341. https://doi.org/10.1586/erm.09.15
  12. Menon, J.U., Kona, S., Wadajkar, A.S., Desai, F., Vadla, A. & Nguyen, K.T. 2012. Effects of surfactants on the properties of PLGA nanoparticles. Journal of Biomedical Materials Research Part A, 100(8): 1998-2005. https://doi.org/10.1002/jbm.a.34040
  13. Müller, R.H. & Jacobs, C. 2002. Buparvaquone mucoadhesive nanosuspension: preparation, optimisation and long-term stability. International Journal of Pharmaceutics, 237(1): 151-161. https://doi.org/10.1016/S0378-5173(02)00040-6
  14. Nakarani, M., Patel, Priyal, Patel, J., Patel, Pankaj, Murthy, R.S.R. & Vaghani, S.S. 2010. Cyclosporine A-Nanosuspension: Formulation, Characterization and In Vivo Comparison with a Marketed Formulation. Scientia Pharmaceutica, 78(2): 345-362. https://doi.org/10.3797/scipharm.0908-12
  15. Patravale, V.B., Date, A.A. & Kulkarni, R.M. 2004. Nanosuspensions: a promising drug delivery strategy. Journal of Pharmacy Pharmacology, 56(7): 827-840. https://doi.org/10.1211/0022357023691
  16. Pezeshki, A., Ghanbarzadeh, B., Mohammadi, M., Fathollahi, I. & Hamishehkar, H. 2014. Encapsulation of Vitamin A Palmitate in Nanostructured Lipid Carrier (NLC)-Effect of Surfactant Concentration on the Formulation Properties. Advanced Pharmaceutical Bulletin, 4(Suppl2): 563-568. https://doi.org/10.5681/apb.2014.083
  17. Sadozai, S.K., Khan, S.A., Karim, N., Becker, D., Steinbrück, N., Gier, S., Baseer, A., Breinig, F., Kickelbick, G. & Schneider, M. 2020. Ketoconazole-loaded PLGA nanoparticles and their synergism against Candida albicans when combined with silver nanoparticles. Journal of Drug Delivery Science and Technology, 56: 101574. https://doi.org/10.1016/j.jddst.2020.101574
  18. Shafique, M., Khan, M.A., Khan, W.S., Maqsood-ur-Rehman, Ahmad, W. & Khan, S. 2017. Fabrication, Characterization, and In Vivo Evaluation of Famotidine Loaded Solid Lipid Nanoparticles for Boosting Oral Bioavailability. Journal of Nanomaterials, 2017, e7357150. https://doi.org/10.1155/2017/7357150
  19. Sharma, N., Madan, P. & Lin, S. 2016. Effect of process and formulation variables on the preparation of parenteral paclitaxel-loaded biodegradable polymeric nanoparticles: A co-surfactant study. Asian Journal of Pharmaceutical Sciences, 11(3): 404-416. https://doi.org/10.1016/j.ajps.2015.09.004
  20. Shi, C., Zeng, F. & Fu, D. 2016. Surfactant-free poly(lactide-co-glycolide) nanoparticles for improving in vitro anticancer efficacy of tetrandrine. Journal of Microencapsulation, 33(3): 249-256. https://doi.org/10.3109/02652048.2016.1156175
  21. Şengel Türk, C.T., Sezgin Bayındır, Z. & Badıllı, U. 2009. Preparation of polymeric nanoparticles using different stabilizing agents. Ankara Universitesi Eczacılık Fakultesi Dergisi, 38(4): 257-268.
  22. Vangijzegem, T., Stanicki, D. & Laurent, S. 2019. Magnetic iron oxide nanoparticles for drug delivery: applications and characteristics. Expert Opinion on Drug Delivery, 16(1): 69-78. https://doi.org/10.1080/17425247.2019.1554647
  23. Wani, I.A. & Ahmad, T., 2013. Size and shape dependant antifungal activity of gold nanoparticles: A case study of Candida. Colloids and Surfaces B: Biointerfaces, 101: 162-170. https://doi.org/10.1016/j.colsurfb.2012.06.005
  24. Water, J.J., Smart, S., Franzyk, H., Foged, C. & Nielsen, H.M. 2015. Nanoparticle-mediated delivery of the antimicrobial peptide plectasin against Staphylococcus aureus in infected epithelial cells. European Journal of Pharmaceutics and Biopharmaceutics, 92: 65-73. https://doi.org/10.1016/j.ejpb.2015.02.009
  25. Yenice Gürsu, B. 2020. Potential antibiofilm activity of farnesol-loaded poly(DL-lactide-co-glycolide) (PLGA) nanoparticles against Candida albicans. Journal of Analytical Science and Technology, 11(1): 43. https://doi.org/10.1186/s40543-020-00241-7
  26. Yi, G., Son, J., Yoo, J., Park, C. & Koo, H. 2019. Rhamnolipid nanoparticles for in vivo drug delivery and photodynamic therapy. Nanomedicine: Nanotechnology, Biology and Medicine, 19: 12-21. https://doi.org/10.1016/j.nano.2019.03.015