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Journals / Trakya University Journal of Natural Sciences / 2026 / Volume: 25 Issue: 1

Effects of gossypin on acetaminophen-induced hepatotoxicity in mice

İrfan Çınar(KASTAMONU ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0002-9826-2556
Muhammed Yayla(KAFKAS ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0002-0659-3084
Erdem Toktay(KAFKAS ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0002-0715-2707
Damla Binnetoğlu(KAFKAS ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0002-7041-7253
Pages
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Publication Date
2024-04-15
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Taken together, GOS has the potential to be devel-oped as a preventive agent to be administered to patients suffering from APAP overdose.

📌 3 Key Takeaways

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Objective & Hypothesis:Liver injury from paracetamol (acetaminophen) (APAP) is common worldwide.
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Key Findings & Data:Protein and mRNA levels of inflammatory, apoptot-ic, and cytochrome factors, including TNF-α, IL-1β, IL-6, NF-kB, and CYP2E1, were ana-lyzed using real-time PCR.
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Conclusion & Impact:Taken together, GOS has the potential to be devel-oped as a preventive agent to be administered to patients suffering from APAP overdose.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Gossypin · Acetaminophen · CYP2E1

Abstract

Liver injury from paracetamol (acetaminophen) (APAP) is common worldwide. To prevent intoxication with a drug with high poisoning, treatment can be made possible with an easily accessible and harmless substance. This study aimed to investigate the hepatoprotective ef-fects of Gossypin (GOS) in mice exposed to an overdose of APAP -the possible mechanism of action. Specifically, serum [alanine aminotransferase (ALT), aspartate transaminase (AST), and hepatic biochemical parameters (glutathione (GSH), malondialdehyde (MDA) and super-oxide dismutase (SOD)] were evaluated. Protein and mRNA levels of inflammatory, apoptot-ic, and cytochrome factors, including TNF-α, IL-1β, IL-6, NF-kB, and CYP2E1, were ana-lyzed using real-time PCR. Pretreatment with GOS significantly reduced APAP-induced he-patic injury via oxidative stress. Along with potent antioxidant activity, GOS promoted APAP hepatic detoxification by regulating AST, ALT, GSH, MDA, and SOD activities and mRNA levels of the cytochrome CYP2E1 gene. The anti-inflammatory activity of GOS in-creases its production. TNF-α, IL-1β and IL-6, through possible NF-kB blockade, are also responsible for its hepatoprotective effect. Taken together, GOS has the potential to be devel-oped as a preventive agent to be administered to patients suffering from APAP overdose.

Özet

Parasetamole (Asetaminofen) (APAP) bağlı karaciğer hasarı dünya çapında yaygındır. Zehir-lenme oranı yüksek bir ilaçla zehirlenmeyi önlemek için kolay ulaşılabilir ve zararsız bir mad-deyle tedavi mümkün kılınabilir. Bu çalışma, aşırı dozda APAP'a maruz kalan farelerde Gossypin (GOS) hepatoprotektif etkilerini (olası etki mekanizması) araştırmayı amaçladı. Spesifik olarak serum [alanin aminotransferaz (ALT), aspartat transaminaz (AST) ve hepatik biyokimyasal parametreler (glutatyon (GSH), malondialdehit (MDA) ve süperoksit dismutaz (SOD)] değerlendirildi. Daha sonra inflamatuar, apoptotik protein ve mRNA seviyeleri değer-lendirildi ve TNF-a, IL-1β, IL-6, NF-kB ve CYP2E1 dahil olmak üzere sitokrom faktörleri, gerçek zamanlı PCR kullanılarak analiz edildi. GOS ile ön tedavi, oksidatif stres ve sentrilo-büler yoluyla APAP'ın neden olduğu karaciğer hasarını önemli ölçüde azalttı. GOS, güçlü antioksidan aktivitesinin yanı sıra, sitokrom CYP2E1 geninin AST, ALT, GSH, MDA ve SOD aktivitelerini ve mRNA seviyelerini düzenleyerek APAP hepatik detoksifikasyonunu destekledi. GOS'in anti-inflamatuar aktivitesi, üretimini artırır. Olası NF-kB blokajı yoluyla TNF-α, IL-1β ve IL-6 da hepatoprotektif etkisinden sorumludur. Birlikte ele alındığında GOS, APAP doz aşımından muzdarip olan hastalara uygulanacak önleyici bir ajan olarak ge-liştirilme potansiyeline sahiptir.

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Cite This Article

Çınar, İ., Yayla, M., Toktay, E., Binnetoğlu, D., *, İ. Ç. (2024). Effects of gossypin on acetaminophen-induced hepatotoxicity in mice. Trakya University Journal of Natural Sciences, Volume: 25 Issue: 1. https://doi.org/10.23902/trkjnat.1410800

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