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Journals / Trakya University Journal of Natural Sciences / 2026 / Volume: 25 Issue: 1

Morphological and molecular identification of Dissingia confusa based on the first record of the species in Türkiye

Şuheda Sümeyye Terman(VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ)0000-0002-1244-9716
Ayten Dizkırıcı(VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ)0000-0002-0578-5092
Mustafa Emre Akçay(VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ, FEN FAKÜLTESİ)0000-0002-9215-3383
Cemil Sadullahoğlu(ŞIRNAK ÜNİVERSİTESİ)0000-0002-0442-9045
Pages
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Publication Date
2024-04-15
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Detailed description, colour images of fresh and dried ascomata, along with photographs of microscopic characters and the obtained phylogenetic trees are given.

📌 3 Key Takeaways

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Objective & Hypothesis:In the present study, specimens kept in Fungarium of Van Yüzüncü Yıl University - Türkiye (VANF) were evaluated based on morphological and molecular characters, and identified as Dissingia confusa as a new record from Eastern Türkiye.
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Key Findings & Data:Ascocarp, hymenium, stipe, asco-spores, ascus and paraphyses were considered as diagnostic morphological characters and the nucleotide sequences of the large subunit (LSU) ribosomal DNA and the translation elonga-tion factor 1-alpha (TEF1-α) were referred as molecular data.
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Conclusion & Impact:Detailed description, colour images of fresh and dried ascomata, along with photographs of microscopic characters and the obtained phylogenetic trees are given.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Dissingia · Helvella · molecular taxonomy

Abstract

In the present study, specimens kept in Fungarium of Van Yüzüncü Yıl University - Türkiye (VANF) were evaluated based on morphological and molecular characters, and identified as Dissingia confusa as a new record from Eastern Türkiye. Ascocarp, hymenium, stipe, asco-spores, ascus and paraphyses were considered as diagnostic morphological characters and the nucleotide sequences of the large subunit (LSU) ribosomal DNA and the translation elonga-tion factor 1-alpha (TEF1-α) were referred as molecular data. A phylogenetic analysis was performed with Maximum parsimony and Bayesian inference methods. The DNA sequences of 15 (7 for TEF1-α) Dissingia and 27 (14 for TEF1-α) Helvella specimens downloaded from the GenBank database were included in the analysis to estimate phylogeny between the two close genera. Morphological evaluations and genetic evidences confirmed that Dissingia is phylogenetically separated from Helvella at genus level and helped to identify the studied specimens as D. confusa as a new record in Türkiye. Detailed description, colour images of fresh and dried ascomata, along with photographs of microscopic characters and the obtained phylogenetic trees are given.

Özet

Van Yüzüncü Yıl Üniversitesi Fungaryumu’nda (VANF) muhafaza edilen Dissingia confusa türü morfolojik ve moleküler karakterler ile Türkiye'nin doğusundan yeni bir kayıt olarak tanımlanmıştır. Şapka, himenyum, sap, spor, askus ve parafiz yapıları morfolojik veri olarak, büyük alt ünite (LSU) ve translasyon uzama faktörü 1-alfa (TEF1-α) bölgelerinin nükleotid dizileri moleküler veri olarak kullanılmıştır. Filogenetik analiz Maksimum parsimoni ve Bayesian çıkarım yöntemi ile gerçekleştirilmiştir. Analizlere, iki cins arasındaki filogeniyi göstermek için GenBank veritabanından indirilen 15 (TEF1-α için 7) Dissingia ve 27 (TEF1-α için 14) Helvella örnekleri de eklenmiştir. Morfolojik çalışmalar ve genetik kanıtlar, Dissingia cinsinin cins düzeyinde Helvella cinsinden filogenetik olarak ayrıldığını ve Dissingia confusa türünün Türkiye'de ilk kez tanımlandığını doğrulamıştır. Detaylı deskripsiyon, taze ve kuru örneklerin renkli fotoğrafları ile mikroskobik karakterlerin görüntüleri ve filogenetik ağaçlar verilmiştir.

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Cite This Article

Terman, Ş. S., Dizkırıcı, A., Akçay, M. E., Sadullahoğlu, C. (2024). Morphological and molecular identification of Dissingia confusa based on the first record of the species in Türkiye. Trakya University Journal of Natural Sciences, Volume: 25 Issue: 1. https://doi.org/10.23902/trkjnat.1379302

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