Journals / Gıda / 2026 / Cilt: 51 Sayı: 1

EFFECT OF SPENT COFFEE GROUND SUBSTITUTION ON PHYSICAL, CHEMICAL, AND SENSORY ATTRIBUTES OF GLUTEN-FREE CAKE

KAHVE TELVESİ ATIĞI İKAMESİNİN GLUTENSİZ KEKLERİN FİZİKSEL, KİMYASAL VE DUYUSAL ÖZELLİKLERİ ÜZERİNE ETKİSİ

Gizem Tiryaki(Ege Üniversitesi Gıda Mühendisliği Bölümü)0000-0001-5608-9815
Journal
Gıda
Pages
91–107
Publication Date
2026-01-31
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Sensory analysis showed that gluten-free cakes with 25 and 50% SCG were most preferred, with higher SCG content decreasing sensory scores (P<0.05).

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Spent coffee grounds (SCG) can increase the technological and nutritional value of foods thanks to its antioxidants and dietary fiber content.
📊
Key Findings & Data:In this study, the physical, chemical and sensory properties of gluten-free cakes produced by substituting rice flour with SCG at 0%, 25%, 50%, 75% and 100% were evaluated.
💡
Conclusion & Impact:Sensory analysis showed that gluten-free cakes with 25 and 50% SCG were most preferred, with higher SCG content decreasing sensory scores (P<0.05).
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Alternatif gıda ürünleri · glütensiz kek · kahve telvesi atığı (KTA)

Abstract

Spent coffee grounds (SCG) can increase the technological and nutritional value of foods thanks to its antioxidants and dietary fiber content. In this study, the physical, chemical and sensory properties of gluten-free cakes produced by substituting rice flour with SCG at 0%, 25%, 50%, 75% and 100% were evaluated. SCG addition significantly increased the phenolic and antioxidant contents and the gluten-free cake with 100% SCG exhibited the highest phenolic (2.70 mg GAE/g), and antioxidant (2.54 mg TE/g for ABTS and 4.17 mg TE/g for DPPH) values (P<0.05). SCG addition affected their hardness and color properties, with higher SCG ratios leading to darker and softer cakes (P<0.05). Also, it affected the baking loss, symmetry, volume, and uniformity index values of cakes. The gluten-free cake with 25% SCG had the highest specific volume (1.68 cm3/g) (P<0.05). Sensory analysis showed that gluten-free cakes with 25 and 50% SCG were most preferred, with higher SCG content decreasing sensory scores (P<0.05).

Özet

Kahve telvesi atığı (KTA), antioksidan ve diyet lifi içeriği sayesinde gıdaların teknolojik ve besin değerini artırmaktadır. Bu çalışmada, pirinç unu %0, %25, %50, %75 ve %100 oranlarında KTA ile ikame edilerek üretilen glutensiz keklerin fiziksel, kimyasal ve duyusal özellikleri değerlendirilmiştir. KTA ilavesi, glutensiz kekin fenolik ve antioksidan içeriklerini önemli ölçüde artırmış ve %100 KTA içeren glutensiz kek en yüksek fenolik (2.70 mg GAE/g) ve antioksidan (ABTS için 2.54 mg TE/g ve DPPH için 4.17 mg TE/g) değerlerini göstermiştir (P<0.05). KTA ilavesi sertlik ve renk özelliklerini etkilemiş, daha yüksek KTA oranları daha koyu ve daha yumuşak keklerin elde edilmesini sağlamıştır (P<0.05). Ayrıca, KTA ikamesi, glutensiz keklerin pişirme kaybı, simetri, hacim ve tek düzelik indeks değerlerini de etkilemiştir. %25 KTA içeren glutensiz kekin en yüksek özgül hacme (1.68 cm3/g) sahip olduğu görülmüştür (P<0.05). Duyusal analiz, %25 ve %50 KTA içeren glutensiz keklerin en çok tercih edildiğini, daha yüksek KTA içeriğinin ise duyusal puanlarını düşürdüğü göstermiştir (P<0.05).

🔖

Cite This Article

Tiryaki, G., Nakilcioğlu, E. (2026). EFFECT OF SPENT COFFEE GROUND SUBSTITUTION ON PHYSICAL, CHEMICAL, AND SENSORY ATTRIBUTES OF GLUTEN-FREE CAKE. Gıda, Cilt: 51 Sayı: 1, 91–107. https://doi.org/10.15237//gida.GD25089

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (92)

  1. AACC (2000). Approved Methods of Analysis. 10th Edition, American Association of Cereal Chemists, St. Paul, MN., USA.
  2. AOAC (2005). Official methods of analysis of AOAC international. 18th Edition, Author, Rockville, MD. Aghajanzadeh S, Sultana A, Mohammad Ziaiifar A, Khalloufi S. (2024). Formation of pores and bubbles and their impacts on the quality attributes of processed foods: a review. Food Research International, 114494.
  3. Agustini, S., Yusya, M.K. (2020). The effect of packaging materials on the physicochemical stability of ground roasted coffee. Current Research on Biosciences and Biotechnology, 1(2), 66-70.
  4. Ahanchi, M., Sugianto, E.C., Chau, A., Khoddami, A. (2024). Quality Properties of Bakery Products and Pasta Containing Spent Coffee Grounds (SCGs): 13(22), 3576.
  5. Ahmed, A.R., Alqahtani, N.K., Ramadan, K.M., Mohamed, H.I., Mahmoud, M.A., Elkatry, H.O. (2023). The bioactive substances in spent black tea and Arabic coffee could improve the nutritional value and extend the shelf life of sponge cake after fortification. ACS omega, 8(37), 33593-33609.
  6. Ahmed, H., Abolore, R.S., Jaiswal, S., Jaiswal, A.K. (2024). Toward circular economy: Potentials of spent coffee grounds in bioproducts and chemical production. Biomass, 4(2), 286- 312.
  7. Aisyah, N., Sunarti, T.C., Meryandini, A. (2025). Antioxidant activity, total phenolic, flavonoid, and caffeine contents of robusta coffee (Coffea canephora) fermented with lactic acid bacteria. Menara Perkebunan, 93(1), 21-30.
  8. Ali, H.S., Mansour, A.F., Kamil, M.M., Hussein, A.M.S. (2018). Formulation of nutraceutical biscuits based on dried spent coffee grounds. Int. J. Pharmacol, 14(4), 584-594.
  9. Alshuaib, F., Baloach, H., Venkatesan, S. (2025). Lower energy consumption-based approach to utilising spent coffee grounds and biochar in cement mortar and concrete. Australian Journal of Civil Engineering, 1-13.
  10. Andrade, C., Perestrelo, R., Câmara, J.S. (2022). Bioactive compounds and antioxidant activity from spent coffee grounds as a powerful approach for its valorization. Molecules, 27(21), 7504.
  11. AOAC (1990). Official Methods of Analysis. 14th Edition, Association of Official Analytical Chemists, Washington, DC.
  12. AOAC (2007). Official Methods of Analysis of AOAC International. 18th Edition, DC: Association of Official Analytical Chemists, Washington, DC.
  13. AOAC (2000). Official Methods of Analysis. 17th Edition, Association of official analytical chemist international, Gaithersburg, MA. Ardeshir, A., Fazeli, F., Khorshidian, N., Mohammadi, M. (2024). Physicochemical, antioxidant, and sensory characteristics of sponge cake fortified with quinoa flour, oolong, and white tea powder. Journal of Food Quality, 2024(1), 6086158.
  14. Arslain, K., Gustafson, C.R., Baishya, P., Rose, D.J. (2021). Determinants of gluten-free diet adoption among individuals without celiac disease or non-celiac gluten sensitivity. Appetite, 156, 104958.
  15. Arámburo-Gálvez, J.G., Beltrán-Cárdenas, C.E., Geralda André, T., Carvalho Gomes, I., Macêdo-Callou, M.A., Braga-Rocha, É.M., & Cabrera-Chávez, F. (2020). Prevalence of Adverse Reactions to Glutenand People Going on a Gluten-Free Diet: A Survey Study Conducted in Brazil. Medicina, 56(4), 163.
  16. Aslan, M., Bilgiçli, N. (2022). The comparison of lupin milk with soy milk as an alternative egg substitute for cake production. Journal of Food Processing and Preservation, 46(5), e16572.
  17. Aslan Türker, D., Göksel Saraç, M., Doğan, M. (2023). Development of gluten-free cake formulations: the role of tapioca & potato starch and quinoa in the rheological, textural and powder flow properties. European Food Research and Technology, 249(3), 675-684.
  18. Ateş, G., Elmacı, Y. (2019). Physical, chemical and sensory characteristics of fiber-enriched cakes prepared with coffee silverskin as wheat flour substitution. Journal of Food Measurement and Characterization, 13(1), 755-763.
  19. Azuan, A.A., Mohd, Z.Z., Hasmadi, M., ND, R., Zainol, M.K. (2020). Physicochemical, antioxidant and sensory characteristics of cookies supplemented with different levels of spent coffee ground extract. Food Research. Ballesteros, L.F., Teixeira, J.A., Mussatto, S.I. (2014). Chemical, functional, and structural properties of spent coffee grounds and coffee silverskin. Food and bioprocess technology, 7, 3493-3503.
  20. Benincá, D.B., do Carmo, L.B., Grancieri, M., Aguiar, L.L., Lima Filho, T., Costa, A.G.V., & Silva, P.I. (2023). Incorporation of spent coffee grounds in muffins: A promising industrial application. Food Chemistry Advances, 3, 100329.
  21. Bevilacqua, E., Cruzat, V., Singh, I., Rose’Meyer, R.B., Panchal, S.K., Brown, L. (2023). The potential of spent coffee grounds in functional food development. Nutrients, 15(4), 994.
  22. Bijla, L., Ibourki, M., Bouzid, H.A., Sakar, E.H., Aissa, R., Laknifli, A., Gharby, S. (2022). Proximate composition, antioxidant activity, mineral and lipid profiling of spent coffee grounds collected in Morocco reveal a great potential of valorization. Waste and Biomass Valorization, 13(11), 4495-4510.
  23. Bulkaini, L.A., Apriyanto, Y., Harja, O., Wijaya, S.R. (2024). Managing Food Waste: Examining The Value and Sen-Sory of Spent Coffee Ground as Ingridient to Making Bread. Jurnal Sains Pariwisata dan Perhotelan, 2(1), 47-58.
  24. Carmen, M.T., Lorena, Z.C., Alexander, V.A., Amandio, V., Raúl, S. (2020). Coffee pulp: An industrial by-product with uses in agriculture, nutrition and biotechnology. Reviews in Agricultural Science, 8, 323-342.
  25. Castaldo, L., Lombardi, S., Gaspari, A., Rubino, M., Izzo, L., Narváez, A., & Grosso, M. (2021). In vitro bioaccessibility and antioxidant activity of polyphenolic compounds from spent coffee grounds-enriched cookies. Foods, 10(8), 1837.
  26. Cavanagh, Q., Brooks, M.S.L., Rupasinghe, H.V. (2023). Innovative technologies used to convert spent coffee grounds into new food ingredients: Opportunities, challenges, and prospects. Future Foods, 8, 100255.
  27. Cemeroğlu, B.S. (2007). Gıda analizleri (Gıda Teknolojisi Derneği). Ankara: Bizim Büro Basımevi. 535 p.
  28. Changotra, R., Yang, J., Rajput, H., Hu, Y., He, Q.S. (2024). Mechanism and performance evaluation of spent-coffee grounds-derived nanocomposite materials for highly efficient photocatalytic degradation of organic pollutant. Journal of Industrial and Engineering Chemistry, 133, 428-438.
  29. Choe, U. (2025). Valorization of spent coffee grounds and their applications in food science. Current Research in Food Science, 101010.
  30. Chrysargyris, A., Antoniou, O., Xylia, P., Petropoulos, S., Tzortzakis, N. (2021). The use of spent coffee grounds in growing media for the production of Brassica seedlings in nurseries. Environmental Science and Pollution Research, 28, 24279-24290.
  31. Çelik, C., ISIK, F. (2023). Quality characteristics of gluten-free muffins fortified with watermelon rind powder. Food Science and Technology, 43, e113822.
  32. Dadalı, C., Elmacı, Y. (2019). Reduction of sucrose by inhomogeneous distribution in cake formulation. Journal of Food Measurement and Characterization, 13(4), 2563-2570.
  33. de la Hera, E., Ruiz-París, E., Oliete, B., Gómez, M. (2012). Studies of the quality of cakes made with wheat-lentil composite flours. LWT, 49(1), 48-54.
  34. Demirkesen, I., Ozkaya, B. (2022). Recent strategies for tackling the problems in gluten-free diet and products. Critical Reviews in Food Science and Nutrition, 62(3), 571-597.
  35. Diego, M. C. J., (2023). The coffee: Overview of the global market, the performance and the business strategies in Colombia, Master of Science Degree in Engineering and Management, Torino, Italy.
  36. Dong, W., Wang, D., Hu, R., Long, Y., Lv, L. (2020). Chemical composition, structural and functional properties of soluble dietary fiber obtained from coffee peel using different extraction methods. Food Research International, 136, 109497.
  37. European Commission (2024). Regulation (EU) 2024/2470 of the European Parliament and of the Council of 1 May 2024 on microbiological criteria for foodstuffs. Official Journal of the European U n i o n. https://eur-lex.europa.eu/legal-content/EN/TXT/HTML/?uri=CELLAR%3A3a3a8edd-0314- 11ef-a251-01aa75ed71a1 (Accessed: 13 July 2025).
  38. Flores, G., Wherley, B., McInnes, K., Feagley, S., Hejl, R. (2024). Evaluation of spent coffee grounds as a nutrient source for turfgrass systems. Journal of Plant Nutrition, 47(19), 3526- 3541.
  39. Franca, A. S., Basílio, E. P., Resende, L. M., Fante, C. A., Oliveira, L. S. (2024). Coffee Silverskin as a Potential Ingredient for Functional Foods: Recent Advances and a Case Study with Chocolate Cake. Foods, 13(23), 3935.
  40. Franca, A. S., Oliveira, L. S. (2022). Potential uses of spent coffee grounds in the food industry. Foods, 11(14), 2064. Garcia, C. V., Kim, Y. T. (2021). Spent coffee grounds and coffee silverskin as potential materials for packaging: A review. Journal of Polymers and the Environment, 29(8), 2372- 2384.
  41. Garcia-Salas, P., Morales-Soto, A., Segura-Carretero, A., Fernández- Gutiérrez, A. (2010). Phenolic-compound-extraction systems for fruit and vegetable samples. Molecules, 15(12), 8813-8826.
  42. González, J. D. T. (2024). Reduction of in Vitro and in Vivo Starch Digestibility in Baked Starchy Matrices Formulated with Soluble Dietary Fiber, Doctoral dissertation, Pontificia Universidad Catolica de Chile (Chile)). Han, Z., Zhu, M., Wan, X., Zhai, X., Ho, C. T., Zhang, L. (2024). Food polyphenols and Maillard reaction: regulation effect and chemical mechanism. Critical reviews in food science and nutrition, 64(15), 4904-4920.
  43. Horgan, F. G., Floyd, D., Mundaca, E. A., Crisol-Martínez, E. (2023). Spent coffee grounds applied as a top-dressing or incorporated into the soil can improve plant growth while reducing slug herbivory. Agriculture, 13(2), 257.
  44. Hu, G. L., Alafifi, J. H., Quan, C. X., Al-Romaima, A., Qiu, M. H. (2025). Exploring the complexities of bitterness: a comprehensive review of methodologies, bitterants, and influencing factors centered around coffee beverage. Critical Reviews in Food Science and Nutrition, 1-17.
  45. Hussein, A., Ali, H., Bareh, G., Farouk, A. (2019). Influence of Spent Coffee Ground as Fiber Source on Chemical, Rheological and Sensory Properties of Sponge Cake. Pakistan Journal of Biological Sciences: PJBS, 22(6), 273-282.
  46. ICO (2023). Annual Review 2022/2023. https://icocoffee.org (Accessed: 10 June 2025).
  47. Jahanbakhshi, R., Ansari, S. (2020). Physicochemical properties of sponge cake fortified by olive stone powder. Journal of Food Quality, 2020(1), 1493638.
  48. Koay, H.Y., Azman, A.T., Zin, Z.M., Portman, K.L., Hasmadi, M., Rusli, N.D., & Zainol, M.K. (2023). Assessing the impact of spent coffee ground (SCG) concentrations on shortbread: A study of physicochemical attributes and sensory acceptance. Future Foods, 8, 100245.
  49. Kreutz, J.M., Adriaanse, M. P., van Der Ploeg, E.M., Vreugdenhil, A. C. (2020). Narrative review: nutrient deficiencies in adults and children with treated and untreated celiac disease. Nutrients, 12(2), 500.
  50. Li, M., Sun, H.N., Mu, T.H., Tian, S.L., Ji, L.L., Zhang, D.Q., & Tian, J.C. (2020). Preparation and quality characteristics of gluten‐free potatocake. Journal of Food Processing and Preservation, 44(11), e14828.
  51. Lin, Y.W., Lu, W.C., Liao, C.H., Huang, P. H. (2024). Innovative coffee filter cups enhance pour-over coffee quality while providing bioactive benefits-Challenges and opportunities. Journal of Agriculture and Food Research, 15, 101024.
  52. Mahmud, M.C., Shellie, R.A., Keast, R. (2020). Unravelling the relationship between aroma compounds and consumer acceptance: Coffee as an example. Comprehensive Reviews in Food Science and Food Safety, 19(5), 2380-2420.
  53. Majzoobi, M., Poor, Z.V., Jamalian, J., Farahnaky, A. (2016). Improvement of the quality of gluten-free sponge cake using different levels and particle sizes of carrot pomace powder. International Journal of Food Science and Technology, 51(6), 1369-1377.
  54. Martínez-Cervera, S., Salvador, A., Sanz, T. (2015). Cellulose ether emulsions as fat replacers in muffins: Rheological, thermal and textural properties. LWT-Food Science and Technology, 63(2), 1083-1090.
  55. Martinez, N. A. (2025). Micronutrient Deficiencies in Celiac Disease: Systematic Review. Martinez-Saez, N., García, A.T., Pérez, I.D., Rebollo-Hernanz, M., Mesías, M., Morales, F.J., & Del Castillo, M.D. (2017). Use of spent coffee grounds as food ingredient in bakery products. Food Chemistry, 216, 114-122.
  56. Mazzola, A.M., Zammarchi, I., Valerii, M.C., Spisni, E., Saracino, I.M., Lanzarotto, F., & Ricci, C. (2024). Gluten-free diet and other celiac disease therapies: Current understanding and emerging strategies. Nutrients, 16(7), 1006. Mudalal, S., Sawafta, K., Zaqdah, M., Jaayssa, R., Saidi, S., Rahhal, B., Ayyad, Z., & Abu- Khalaf, N. (2025).
  57. Sustainable Cookies Enriched with Spent Coffee Grounds: A Study on Nutritional, Textural, and Sensory Properties. Journal of Food Processing and Preservation, 2025(1), 7439017. Mussatto, S.I., Machado, E.M.S., Martins, S., Teixeira, J.A. (2011). Production, composition, and application of coffee and its industrial residues. Food and Bioprocess Technology, 4(5), 661–672.
  58. Nakilcioglu, E., Dadalı, C. (2024). Optimization of gluten-free cake formulation by replacement of rice flour and baking powder with different levels of eggshell powder using response surface methodology. Journal of Food Measurement and Characterization, 18(7), 5541-5551.
  59. Nehlig, A. (2022). Effects of coffee on the gastro-intestinal tract: a narrative review and literature update. Nutrients, 14(2), 399.
  60. Papageorgiou, C., Dermesonlouoglou, E., Tsimogiannis, D., Taoukis, P. (2024). Enrichment of Bakery Products with Antioxidant and Dietary Fiber Ingredients Obtained from Spent Coffee Ground. Applied Sciences, 14(16), 6863.
  61. Patil, S., Shankar, S.R., Murthy, P.S. (2023). Impact of different varieties and mature stages on phytochemicals from Coffea arabica and Coffea robusta leaves. Biochemical Systematics and Ecology, 110, 104699.
  62. Prihadi, A.R., Maimulyanti, A. (2021). Chemical compounds of coffee ground and spent coffee ground for pharmaceutical products. Pharmaceutical and Biomedical Sciences Journal (PBSJ), 2(2), 49-52.
  63. Procida, G., Lagazio, C., Cateni, F., Zacchigna, M., Cichelli, A. (2020). Characterization of Arabica and Robusta volatile coffees composition by reverse carrier gas headspace gas chromatography– mass spectrometry based on a statistical approach. Food science and biotechnology, 29, 1319-1330.
  64. Qi, K., Cao, S., Li, C. (2024). Possible interaction between pectin and gluten alters the starch digestibility and texture of wheat bread. International Journal of Biological Macromolecules, 269, 131907.
  65. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice- Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine, 26(9- 10), 1231-1237.
  66. Romauli, N.D.M., Siahaan, F.T.K., Sagala, M., Sihombing, H.V., Ambarita, H., Manurung, H. (2023). Comparative investigation on the nutritional value of fresh coffee pulp, cascara powder, and cascara sap from arabica, robusta, and liberica coffee. In IOP Conference Series: Earth and Environmental Science (Vol. 1230, No. 1, p. 012153). IOP Publishing.
  67. Sabença, C., Ribeiro, M., Sousa, T.D., Poeta, P., Bagulho, A.S., Igrejas, G. (2021). Wheat/glutenrelated disorders and gluten-free diet misconceptions: A review. Foods, 10(8), 1765.
  68. Saberian, M., Li, J., Donnoli, A., Bonderenko, E., Oliva, P., Gill, B., & Siddique, R. (2021). Recycling of spent coffee grounds in construction materials: A review. Journal of Cleaner Production, 289, 125837.
  69. Salazar‐López, N.J., López‐Rodríguez, C.V., Hernández‐Montoya, D.A., Campos‐Vega, R. (2020). Health benefits of spent coffee grounds. Food Wastes and By‐products: Nutraceutical and Health Potential, 327-351.
  70. Saratale, G.D., Bhosale, R., Shobana, S., Banu, J.R., Pugazhendhi, A., Mahmoud, E., & Kumar, G. (2020). A review on valorization of spent coffee grounds (SCG) towards biopolymers and biocatalysts production. Bioresource technology, 314, 123800.
  71. Saxena, R., Laddha, H., Bhoi, R. G. (2025). Sustainable management of spent coffee grounds: applications, decompositions techniques and structural analysis. Journal of Material Cycles and Waste Management, 27(1), 19-33.
  72. Schages, J., Brands, B., Heldt, P., Kohlmann, R., Pickel, A., Bockmühl, D. (2018). The influence of technical and microbiological parameters on the hygienic quality of coffee from fully automated coffee machines. Journal of Bioprocessing & Biotechniques, (8).
  73. Schakel, S.F., Jasthi, B., Van Heel, N., Harnack, L. (2009). Adjusting a nutrient database to improve calculation of percent calories from macronutrients. Journal of food composition and analysis, 22, S32-S36.
  74. Singla, D., Malik, T., Singh, A., Thakur, S., Kumar, P. (2024). Advances in understanding wheat-related disorders: A comprehensive review on gluten-free products with emphasis on wheat allergy, celiac and non-celiac gluten sensitivity. Food Chemistry Advances, 4, 100627.
  75. Sirichokworrakit, S., Aukkanit, N., Sukatta, U., Chutrtong, J., Sangsuwon, C. (2024). Physicochemical and antioxidant properties of gluten-free chiffon cake prepared with riceberry rice flour as replacement for rice flour. International Food Research Journal, 31(4), 886-895.
  76. Sulyman, M., Al-Marog, S., Al-Azabi, K., Dawed, E., Abukrain, A. (2020). Economical and Eco-Friendly Adsorbent Derived from Coffee Waste for Efficient Adsorption of Methylene Blue: Characterization, Evaluation and Optimization Studies. Chemical Science International Journal, 16–36. Sun, T., Powers, J. R., Tang, J. (2007). Evaluation of the antioxidant activity of asparagus, broccoli and their juices. Food chemistry, 105(1), 101-106.
  77. Trà, T.T.T., Yến, V.T.N., Thu, N.T.A., Nguyệt, T.N.M., Mẫn, L.V.V. (2021). Use of wheat flour and spent coffee grounds in the production of cookies with high fiber and antioxidant content: Effects of spent coffee grounds ratio on the product quality. In IOP Conference Series: Earth and Environmental Science (Vol. 947, No. 1, p. 012044). IOP Publishing.
  78. Turkish Food Codex Microbiological Criteria Regulation (No. 2022/4693).
  79. https://www.denib.gov.tr/files/downloads/sirku_ ekleri/2022-298-ek1.pdf (access: 08.12.2025).
  80. Türk Gıda Kodeksi (2011). Mikrobiyolojik Kriterler Yönetmeliği, Tarım ve Köy İşleri Bakanlığı, 23960, Tebliğ No:2001-19, Ankara, Türkiye.
  81. Türker, B., Savlak, N. (2022). Gluten-free cake with unripe banana peel flour substitution: impact on nutritional, functional and sensorial properties. Nutrition & Food Science, 52(6), 980-995.
  82. Wieser, H., Ruiz-Carnicer, Á., Segura, V., Comino, I., Sousa, C. (2021). Challenges of monitoring the gluten-free diet adherence in the management and follow-up of patients with celiac disease. Nutrients, 13(7), 2274.
  83. Wu, H., Gu, J., Amrit, B.K., Nawaz, M.A., Barrow, C.J., Dunshea, F.R., Suleria, H.A. (2022). Effect of processing on bioaccessibility and bioavailability of bioactive compounds in coffee beans. Food Bioscience, 46, 101373.
  84. Xu, B.J. Chang, S.K., 2007, A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents, J Food Sci, 72:S159–166 pp.
  85. Yang, Y., Liu, Q., Yue, F. (2024). Glycemic Response in Nonhuman Primates Fed Gluten- Free Rice Cakes Enriched with Soy, Pea, or Rice Protein and Its Correlation with Nutrient Composition. Nutrients, 16(2), 234.
  86. Yusufoğlu, B., Kezer, G., Wang, Y., Ziora, Z.M., Esatbeyoglu, T. (2024). Bio-recycling of spent coffee grounds: Recent advances and potential applications. Current Opinion in Food Science, 55, 101111.
  87. Zabaniotou, A., Kamaterou, P. (2019). Food waste valorization advocating Circular Bioeconomy-A critical review of potentialities and perspectives of spent coffee grounds biorefinery. Journal of cleaner production, 211, 1553-1566.
  88. Zhao, N., Liu, Z., Yu, T., Yan, F. (2024). Spent coffee grounds: Present and future of environmentally friendly applications on industries-A review. Trends in Food Science & Technology, 143, 104312.
  89. Zhuang, J., Zhu, J., Cheung, P.C., Li, C. (2024). The physical and chemical interactions between starch and dietary fiber: Their impact on the physicochemical and nutritional properties of starch. Trends in Food Science & Technology, 104566.
  90. Zuorro, A., Lavecchia, R. (2012). Spent coffee grounds as a valuable source of phenolic compounds and bioenergy. Journal of Cleaner Production, 34, 49–56.
  91. Agustini, S., Yusya, M.K. (2020). The effect of packaging materials on the physicochemical stability of ground roasted coffee. Current Research on Biosciences and Biotechnology, 1(2), 66-70.
  92. European Commission (2024). Regulation (EU) 2024/2470 of the European Parliament and of the Council of 1 May 2024 on microbiological criteria for foodstuffs. Official Journal of the European U n i o n. https://eur-lex.europa.eu/legal-content/EN/TXT/HTML/?uri=CELLAR%3A3a3a8edd-0314- 11ef-a251-01aa75ed71a1 (Accessed: 13 July 2025).