Journals / Gıda / 2026 / Cilt: 51 Sayı: 4

EFFECTS OF GRAPEFRUIT PEEL (Citrus paradisi) EXTRACT ADDITION ON THE OXIDATIVE STABILITY, PHYSICOCHEMICAL AND TEXTURAL PROPERTIES OF RAW STORED BEEF HAMBURGER PATTIES

GREYFURT KABUĞU (Citrus paradisi) EKSTRAKTI İLAVESİNİN ÇİĞ DEPOLANMIŞ DANA HAMBURGER KÖFTELERİN OKSİDATİF STABİLİTESİ, FİZİKOKİMYASAL VE TEKSTÜREL ÖZELLİKLERİ ÜZERİNE ETKİLERİ

Azim Şimşek(ISPARTA UYGULAMALI BİLİMLER ÜNİVERSİTESİ)0000-0002-7570-0832
Journal
Gıda
Pages
771–788
Publication Date
2026-07-31
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results demonstrated that GKE possesses natural antioxidant potential for improving oxidative stability, with its protective effects being further enhanced with increasing concentrations of GKE.

📌 3 Key Takeaways

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Objective & Hypothesis:This study investigated the effects of lyophilized grapefruit peel extract (GKE) and BHT on the oxidative stability, physicochemical, and textural characteristics of raw beef patties during refrigerated storage for 6 days.
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Key Findings & Data:A total of four experimental groups were established within the scope of the research: a control group containing no additives, a group supplemented with 0.01% BHT as a synthetic antioxidant, and two groups treated with 0.5% and 1.0% GKE to evaluate the efficacy of the natural antioxidant at different concentrations.
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Conclusion & Impact:The results demonstrated that GKE possesses natural antioxidant potential for improving oxidative stability, with its protective effects being further enhanced with increasing concentrations of GKE.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Hamburger köfte · bütillenmiş hidroksitoluen · greyfurt kabuğu

Abstract

This study investigated the effects of lyophilized grapefruit peel extract (GKE) and BHT on the oxidative stability, physicochemical, and textural characteristics of raw beef patties during refrigerated storage for 6 days. A total of four experimental groups were established within the scope of the research: a control group containing no additives, a group supplemented with 0.01% BHT as a synthetic antioxidant, and two groups treated with 0.5% and 1.0% GKE to evaluate the efficacy of the natural antioxidant at different concentrations. The incorporation of GKE significantly limited both protein and lipid oxidation by reducing lipid hydroperoxide (LPO), thiobarbituric acid reactive substances (TBARS), and carbonyl contents compared to control and BHT (P<0.05). Furthermore, the use of GKE led to a decrease in pH, hardness, and gumminess values, while it resulted in an increase in L*, a*, and b* color values (P<0.05). In contrast, no significant changes were generally observed in the chemical composition or other textural characteristics (P>0.05). The results demonstrated that GKE possesses natural antioxidant potential for improving oxidative stability, with its protective effects being further enhanced with increasing concentrations of GKE.

Özet

Bu çalışmada, çiğ dana hamburger köftelerinde liyofilize greyfurt kabuğu ekstraktı (GKE) ve bütillenmiş hidroksitoluen (BHT) kullanımının oksidatif stabilite, fizikokimyasal ve tekstürel özellikler üzerindeki etkileri 6 günlük soğuk depolama (4℃) süresince incelenmiştir. Araştırma kapsamında; herhangi bir katkı içermeyen kontrol grubu, sentetik antioksidan içeren %0.01 BHT grubu ve doğal antioksidan etkisini test etmek amacıyla iki farklı konsantrasyonda hazırlanan %0.5 GKE ile %1.0 GKE grupları olmak üzere toplam dört farklı deneme grubu oluşturulmuştur. GKE ilavesi, kontrol ve BHT gruplarına kıyasla lipit hidroperoksit (LPO), tiyobarbitürik asit reaktif maddeler (TBARS) ve karbonil değerlerini önemli ölçüde düşürerek (P<0.05) protein ve lipit oksidasyonunu sınırlandırmıştır. Ayrıca GKE kullanımı pH, sertlik ve sakızımsılık değerlerinde düşüşe; L*, a* ve b* renk değerlerinde ise artışa neden olmuştur (P<0.05). Kimyasal kompozisyon ve diğer tekstürel özellikler üzerinde ise genel olarak belirgin bir değişim gözlenmemiştir (P>0.05). Sonuçlar, GKE’nin oksidatif stabiliteyi iyileştirmede etkili bir doğal antioksidan potansiyeline sahip olduğunu ve GKE konsantrasyonu arttıkça antioksidan etkinin artış gösterdiğini ortaya koymuştur.

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Cite This Article

Şimşek, A. (2026). EFFECTS OF GRAPEFRUIT PEEL (Citrus paradisi) EXTRACT ADDITION ON THE OXIDATIVE STABILITY, PHYSICOCHEMICAL AND TEXTURAL PROPERTIES OF RAW STORED BEEF HAMBURGER PATTIES. Gıda, Cilt: 51 Sayı: 4, 771–788. https://doi.org/10.15237/gida.GD26042

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References (49)

  1. Abd El-Khalek, H. H. & Zahran, D. A. (2013). Utilization of fruit by-product in ground meat preservation. Food Science and Quality Management, 11, 49-60.
  2. Abdel-Naeem, H. H., Elshebrawy, H. A., Imre, K., Morar, A., Herman, V., Pa℃calău, R. & Sallam, K. I. (2022). Antioxidant and antibacterial effect of fruit peel powders in chicken patties. Foods, 11(3), 301.
  3. AOAC (1990). Official Methods of Analyses. Association of Official Analytical Chemist. IAC, Arlington, VA, USA. AOAC. (1997). Association of official analytical chemists. In W. Horwitz(Ed.), Official methods of analysis (15th ed.). AOAC International.
  4. Amaral, A. B., Silva, M. V. D. & Lannes, S. C. D. S. (2018). Lipid oxidation in meat: mechanisms and protective factors–a review. Food Science and Technology, 38(Suppl. 1), 1-15.
  5. Ashour, M. M. S., Moawad, R. K. & Bareh, G. F. (2013). Quality enhancement and shelf-life extension of raw beef patties formulated with lactate/thyme essential oil during refrigerated storage. Journal of Applied Sciences Research, 9(13), 6699-6709.
  6. Ashraf, H., Iahtisham-Ul-Haq, Butt, M. S., Nayik, G. A., Ramniwas, S., Damto, T. & Ansari, M. J. (2024). Phytochemical and antioxidant profile of citrus peel extracts in relation to different extraction parameters. International Journal of Food Properties, 27(1), 286-299.
  7. Azmir, J., Zaidul, I. S. M., Rahman, M. M., Sharif, K. M., Mohamed, A., Sahena, F. & Omar, A. K. (2013). Techniques for extraction of bioactive compounds from plant materials: A review. Journal of Food Engineering, 117(4), 426-436.
  8. Babaoğlu, A. S., Ainiwaer, T., Özkan, H. & Karakaya, M. (2022). Grapefruit and pomelo peel extracts as natural antioxidants for improved storage stability of Turkey patties during refrigerated storage. Journal of Food Science and Technology, 59(10), 4067-4074.
  9. Bae, S. M., Yoo, Y., Park, J., Kim, M. & Jeong, J. Y. (2025). Citrus peel extract powders as reducing agents for naturally cured pork sausages: Effects on cured color development. Foods, 14(8), 1397.
  10. Baltar, J. D., Baptista, R. F., de Alcântara Salim, A. P. A., de Oliveira, J. M. G. & Monteiro, M. L. G. (2019). Oxidative stability of lamb meat patties from Longissimus dorsi muscle stored under refrigeration. Journal of Food Studies, 8(1), 2166-1073.
  11. Bambeni, T., Tayengwa, T., Chikwanha, O. C., Manley, M., Gouws, P. A., Marais, J. & Mapiye, C. (2021). Biopreservative efficacy of grape (Vitis vinifera) and clementine mandarin orange (Citrus reticulata) by-product extracts in raw ground beef patties. Meat Science, 181, 108609.
  12. Barriuso, B., Astiasarán, I. & Ansorena, D. (2013). A review of analytical methods measuring lipid oxidation status in foods: a challenging task. European Food Research and Technology, 236(1), 1-15.
  13. Bonny, D. S. S., Li, X., Li, Z., Li, M., Du, M., Gao, L. & Zhang, D. (2017). Colour stability and lipid oxidation of beef Longissimus lumborum under different packaging conditions. Polish Journal of Food and Nutrition Sciences, 67(4).
  14. Caine, W. R., Aalhus, J. L., Best, D. R., Dugan, M. E. R. & Jeremiah, L. E. (2003). Relationship of texture profile analysis and Warner- Bratzler shear force with sensory characteristics of beef rib steaks. Meat Science, 64(4), 333–339.
  15. Castro-Vazquez, L., Alañón, M. E., Rodríguez-Robledo, V., Pérez-Coello, M. S., Hermosín-Gutierrez, I., Díaz-Maroto, M. C. & Arroyo-Jimenez, M. D. M. (2016). Bioactive flavonoids, antioxidant behaviour, and cytoprotective effects of dried grapefruit peels (Citrus paradisi Macf.). Oxidative Medicine and Cellular Longevity, 2016(1), 8915729.
  16. Chappalwar, A. M., Pathak, V., Goswami, M., Verma, A. K. & Rajkumar, V. (2021). Efficacy of lemon albedo as fat replacer for development of ultra℃low℃fat chicken patties. Journal of Food Processing and Preservation, 45(7), e15587.
  17. Disha, M. N. A., Hossain, M. A., Kamal, M. T., Rahman, M. M. & Hashem, M. A. (2020). Effect of different level of lemon extract on quality and shelf life of chicken meatballs during frozen storage. SAARC Journal of Agriculture, 18(2), 139-156.
  18. Domínguez, R., Pateiro, M., Gagaoua, M., Barba, F. J., Zhang, W. & Lorenzo, J. M. (2019). A comprehensive review on lipid oxidation in meat and meat products. Antioxidants, 8(10), 429.
  19. Dua, S., Bhat, Z. F. & Kumar, S. (2015). Effect of lemon peel extract on the oxidative stability and storage quality of Tabak-Maz, traditional fried mutton ribs. Nutrition & Food Science, 45(5), 662-676.
  20. Estévez, M. (2011). Protein carbonyls in meat systems: A review. Meat Science, 89(3), 259-279.
  21. Fernandez-Lopez, J., Fernández-Ginés, J. M., Aleson-Carbonell, L., Sendra, E., Sayas-Barberá, E. & Pérez-Alvarez, J. A. (2004). Application of functional citrus by-products to meat products. Trends in Food Science & Technology, 15(3-4), 176-185.
  22. Garcia-Castello, E. M., Rodriguez-Lopez, A. D., Mayor, L., Ballesteros, R., Conidi, C. & Cassano, A. (2015). Optimization of conventional and ultrasound assisted extraction of flavonoids from grapefruit (Citrus paradisi L.) solid wastes. LWT-Food Science and Technology, 64(2), 1114-1122.
  23. Gulcin, İ. (2025). Antioxidants: a comprehensive review. Archives of Toxicology, 99(5), 1893-1997.
  24. Haque, F., Rahman, M. H., Habib, M., Alam, M. S., Monir, M. M. & Hossain, M. M. (2020). Effect of different levels of orange peel extract on the quality and shelf life of beef muscle during frozen storage. IOSR Journal of Agriculture and Veterinary Science, 13, 43-56.
  25. Huang, R., Zhang, Y., Shen, S., Zhi, Z., Cheng, H., Chen, S. & Ye, X. (2020). Antioxidant and pancreatic lipase inhibitory effects of flavonoids from different citrus peel extracts: An in vitro study. Food Chemistry, 326, 126785.
  26. Ibrahim, H. M., Hassan, I. M. & Hamed, A. A. (2018). Application of lemon and orange peels in meat products: Quality and safety. International Journal of Current Microbiology and Applied Sciences, 7(4), 2703-2723.
  27. Islam, M., Malakar, S., Dwivedi, U., Kumar, N., Prabakar, P. K., Kishore, A. & Kumar, A. (2023). Impact of different drying techniques on grapefruit peels and subsequent optimization of ultrasonic extraction conditions for bioactive compounds. Journal of Food Process Engineering, 46(6), e14331.
  28. Jo, C., Kang, H. J., Lee, M., Lee, N. Y. & Byun, M. W. (2004). The antioxidative potential of lyophilized citrus peel extract in different meat model systems during storage at 20C. Journal of Muscle Foods, 15(2), 95-107.
  29. Jung, Y., Oh, S., Lee, S., Lee, H. J., Choo, H. J., Jo, C., Nam, K., Lee, J. & Jang, A. (2025). Characterization of meat quality, storage stability, flavor-related compounds, and their relationship in Korean Woorimatdag No. 2 chicken breast meat during cold storage. Poultry Science, 104(1), 104566.
  30. Kılıç, B., Şimşek, A., Claus, J. R. & Atılgan, E. (2014). Encapsulated phosphates reduce lipid oxidation in both ground chicken and ground beef during raw and cooked meat storage with some influence on color, pH, and cooking loss. Meat Science, 97(1), 93-103.
  31. Kılıç, B., Şimşek, A., Claus, J. R., Karaca, E. & Bilecen, D. (2018). Improving lipid oxidation inhibition in cooked beef hamburger patties during refrigerated storage with encapsulated polyphosphate incorporation. LWT-Food Science and Technology, 92, 290-296.
  32. Klangpetch, W., Phromsurin, K., Hannarong, K., Wichaphon, J. & Rungchang, S. (2016). Antibacterial and antioxidant effects of tropical citrus peel extracts to improve the shelf life of raw chicken drumettes. International Food Research Journal, 23(2), 700.
  33. Koutsopoulos, D. A., Koutsimanis, G. E. & Bloukas, J. G. (2008). Effect of carrageenan level and packaging during ripening on processing and quality characteristics of low-fat fermented sausages produced with olive oil. Meat Science, 79(1), 188-197.
  34. Liu, F., Xu, Q., Dai, R. & Ni, Y. (2015). Effects of natural antioxidants on colour stability, lipid oxidation and metmyoglobin reducing activity in raw beef patties. Acta Scientiarum Polonorum Technologia Alimentaria, 14(1), 37-44.
  35. Liu, C., Xu, S., Liu, X., Wang, W., Liao, W., Yang, X. & He, Q. (2025). Preservation of beef with limonene-rich citrus peel extracts: Antioxidant, antimicrobial and textural benefits. Foods, 14(20), 3506.
  36. Lund, M. N., Heinonen, M., Baron, C. P. & Estévez, M. (2011). Protein oxidation in muscle foods: A review. Molecular Nutrition & Food Research, 55(1), 83-95.
  37. Min, B. & Ahn, D. U. (2005). Mechanism of lipid peroxidation in meat and meat products-A Review. Food Science and Biotechnology, 14(1): 152-163.
  38. Nishad, J., Koley, T. K., Varghese, E. & Kaur, C. (2018). Synergistic effects of nutmeg and citrus peel extracts in imparting oxidative stability in meat balls. Food Research International, 106, 1026-1036.
  39. Nupur, A. H., Mazumder, M. A. R., Karmoker, P. & Alim, M. A. (2022). Effectiveness of orange peel extract on the quality of minced beef during frozen storage. Journal of Food Processing and Preservation, 46(7), e16659.
  40. Sayari, N., Sila, A., Balti, R., Abid, E., Hajlaoui, K., Nasri, M. & Bougatef, A. (2015). Antioxidant and antibacterial properties of Citrus paradisi barks extracts during turkey sausage formulation and storage. Biocatalysis and Agricultural Biotechnology, 4(4), 616-623.
  41. Shantha, N. C. & Decker E. A. (1994). Rapid, sensitive, iron-based spectrophotometric methods for determination of lipid hydroperoxides of food lipids. Journal of AOAC International, 77, 421-424.
  42. Sharedeh, D., Gatellier, P., Astruc, T. & Daudin, J. D. (2015). Effects of pH and NaCl levels in a beef marinade on physicochemical states of lipids and proteins and on tissue microstructure. Meat Science, 110, 24-31.
  43. Soladoye, O. P., Juárez, M. L., Aalhus, J. L., Shand, P. & Estévez, M. (2015). Protein oxidation in processed meat: Mechanisms and potential implications on human health. Comprehensive Reviews in Food Science and Food Safety, 14(2), 106-122.
  44. Şimşek, A. ve Kılıç, B. (2012). Et ve et ürünlerinde antioksidan kullanımındaki güncel gelişmeler. Akademik Gıda, 10(2), 75-83.
  45. Tayengwa, T., Chikwanha, O. C., Gouws, P., Dugan, M. E., Mutsvangwa, T. & Mapiye, C. (2020). Dietary citrus pulp and grape pomace as potential natural preservatives for extending beef shelf life. Meat Science, 162, 108029.
  46. Trindade, R. A., Mancini-Filho, J. & Villavicencio, A. L. C. H. (2010). Natural antioxidants protecting irradiated beef burgers from lipid oxidation. LWT-Food Science and Technology, 43(1), 98-104.
  47. Xu, Y., Li, G., Mo, L., Li, M., Luo, J., Shen, Q. & Quan, W. (2024). Citrus peel extracts: Effective inhibitors of heterocyclic amines and advanced glycation end products in grilled pork meat patties. Foods, 13 (1), 114.
  48. Wazir, H., Chay, S. Y., Ibadullah, W. Z. W., Zarei, M., Mustapha, N. A. & Saari, N. (2021). Lipid oxidation and protein co-oxidation in ready-to-eat meat products as affected by temperature, antioxidant, and packaging material during 6 months of storage. RSC Advances, 11(61), 38565-38577.
  49. Wedamulla, N. E., Fan, M., Choi, Y. J. & Kim, E. K. (2022). Citrus peel as a renewable bioresource: Transforming waste to food additives. Journal of Functional Foods, 95, 105163.