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Journals / Bitki Koruma Bülteni / 2026 / Cilt: 66 Sayı: 3

Identification of soil-borne fungi in wheat rotation fields and biocontrol potential of Clonostachys rosea

Buğday münavebe alanlarındaki toprak kökenli fungusların tanımlanması ve Clonostachys rosea’ nın biyolojik mücadele potansiyeli

Durmuş Erdurmuş(Ankara Plant Protection Central Research Institute)0000-0001-6571-6913
Zeynep Yumlu(SIVAS SCIENCE AND TECHNOLOGY UNIVERSITY)0009-0005-8525-5962
Bayram Kansu(ONDOKUZ MAYIS UNIVERSITY, SAMSUN VOCATIONAL SCHOOL)0000-0001-5663-0528
Emre Evlice(SIVAS SCIENCE AND TECHNOLOGY UNIVERSITY)0000-0001-6402-0287
Pages
232–244
Publication Date
2026-09-30
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These findings suggest that wheat ecosystems may host beneficial antagonists, such as C. rosea, which could contribute to the development of biologically based integrated disease management strategies.

📌 3 Key Takeaways

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Objective & Hypothesis:Fungal diseases represent a major constraint on global wheat production, necessitating the development of sustainable management strategies that integrate biological control agents.
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Key Findings & Data:A total of 19 fungal isolates were recovered from 25 soil samples and identified via ITS rDNA sequencing as C. rosea (6), Fusarium equiseti (6), Fusarium oxysporum (4), Fusarium redolens (1), Alternaria alternata (1), and Alternaria atra (1).
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Conclusion & Impact:These findings suggest that wheat ecosystems may host beneficial antagonists, such as C. rosea, which could contribute to the development of biologically based integrated disease management strategies.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Entegre hastalık yönetimi · ikili kültür · Fusarium culmorum

Abstract

Fungal diseases represent a major constraint on global wheat production, necessitating the development of sustainable management strategies that integrate biological control agents. This study aimed to characterize the culturable fungal community in wheat rotation systems (wheat, barley, safflower, chickpea, lentil, and vetch) in Haymana (Ankara, Türkiye) and to evaluate the antagonistic potential of indigenous Clonostachys rosea isolates. A total of 19 fungal isolates were recovered from 25 soil samples and identified via ITS rDNA sequencing as C. rosea (6), Fusarium equiseti (6), Fusarium oxysporum (4), Fusarium redolens (1), Alternaria alternata (1), and Alternaria atra (1). To assess biocontrol efficacy, three representative C. rosea isolates were screened against the virulent pathogen Fusarium culmorum using dual-culture assays. All tested C. rosea isolates significantly inhibited the radial growth of F. culmorum. The highest inhibition rate was achieved by isolate CR11 (32.19%), followed by CR14 (26.82%) and CR15 (15.94%). These findings suggest that wheat ecosystems may host beneficial antagonists, such as C. rosea, which could contribute to the development of biologically based integrated disease management strategies.

Özet

Fungal patojenler, buğday üretiminde verim ve kaliteyi kısıtlayan en önemli faktörler arasında yer almaktadır. Sürdürülebilir tarım ilkeleri doğrultusunda, kimyasal girdilerin azaltılması için yerel biyolojik mücadele ajanlarının belirlenmesi büyük önem taşımaktadır. Bu çalışmada, Ankara (Haymana) lokasyonundaki buğday ekim nöbeti (buğday, arpa, aspir, nohut, mercimek ve fiğ) deneme alanlarından toplanan toprak örneklerindeki fungal flora karakterize edilmiş ve Clonostachys rosea izolatlarının biyokontrol potansiyeli in vitro koşullarda test edilmiştir. Toplam 25 toprak örneğinden 19 fungal izolat elde edilmiştir; ITS rDNA dizi analizi sonucunda izolatlar C. rosea (6), Fusarium equiseti (6), Fusarium oxysporum (4), Fusarium redolens (1), Alternaria alternata (1) ve Alternaria atra (1) olarak teşhis edilmiştir. Biyolojik mücadele çalışmaları kapsamında, üç farklı C. rosea izolatının önemli bir buğday patojeni olan Fusarium culmorum üzerindeki antagonistik aktivitesi ikili kültür testleriyle değerlendirilmiştir. Deneme sonucunda tüm C. rosea izolatlarının patojenin misel gelişimini farklı oranlarda inhibe ettiği belirlenmiştir. En yüksek engelleme oranı %32,19 ile CR11 izolatında saptanırken, bunu %26,82 ile CR14 ve %15,94 ile CR15 izolatları izlemiştir. Bu bulgular, buğday ekosistemlerinde C. rosea gibi potansiyel olarak faydalı antagonistlere ev sahipliği yapabileceğini ve bunun da gelecekte biyolojik tabanlı entegre hastalık yönetimi stratejilerinin geliştirilmesine katkıda bulunabileceğini göstermektedir.

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Cite This Article

Erdurmuş, D., Yumlu, Z., Kansu, B., Evlice, E. (2026). Identification of soil-borne fungi in wheat rotation fields and biocontrol potential of Clonostachys rosea. Bitki Koruma Bülteni, Cilt: 66 Sayı: 3, 232–244. https://doi.org/10.16955/bitkorb.1914955

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