According to the results obtained, no polymorphism was detected among the micropropagated plants.
📌 3 Key Takeaways
🎯
Objective & Hypothesis:Determination of the genetic characters of the plants obtained using plant tissue culture methods is important.
📊
Key Findings & Data:In this study, the genetic fidelity of the plants, which are obtained by the micropropagation of the shoot tip explants of the Origanum majorana L. plant of medicinal and economical value in Murashige and Skoog (MS) medium which contains 1.0 mg/L Benzil Amino Purin (BAP), 0.1 mg/L naphthaleneacetic acid (NAA), 3% sucrose and 0.7% agar, has been investigated by using random amplified polymorphic DNA (RAPD-PCR) technique.
💡
Conclusion & Impact:According to the results obtained, no polymorphism was detected among the micropropagated plants.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Genetic fidelity · Medicinal herb · Micropropagation
Abstract
Determination of the genetic characters of the plants obtained using plant tissue culture methods is important. In this study, the genetic fidelity of the plants, which are obtained by the micropropagation of the shoot tip explants of the Origanum majorana L. plant of medicinal and economical value in Murashige and Skoog (MS) medium which contains 1.0 mg/L Benzil Amino Purin (BAP), 0.1 mg/L naphthaleneacetic acid (NAA), 3% sucrose and 0.7% agar, has been investigated by using random amplified polymorphic DNA (RAPD-PCR) technique. Monomorphic bands were obtained as a result of all of the RAPD-PCR analyses performed. According to the results obtained, no polymorphism was detected among the micropropagated plants.
ÇETİN, B. (2018). Evaluation of the Genetic Fidelity of in vitro Raised Plants of Origanum majorana L. Using Random Amplified Polymorphic DNA. Celal Bayar Üniversitesi Fen Bilimleri Dergisi, Cilt: 14 - Sayı: 2, 237–239. https://ajindex.com/articles/109860
Avcı, B, Bayram, E, Geliştirilmiş İzmir Kekiği (Origanum Onites L.) klonlarının farklı ekolojik koşullarda bazı agronomik ve teknolojik özelliklerinin belirlenmesi, Journal of Agriculture Faculty of Ege University, 2013, 50(1), 13-20
Hajlaoui, H, Mighri, H, Aouni, M, Gharsallah, N, Kadri, A, Chemical composition and in vitro evaluation of antioxidant, antimicrobial, cytotoxicity and anti-acetylcholinesterase prop-erties of Tunisian Origanum majorana L. essential oil, Microbial Pathogenesis, 2016, 95, 86-94
Hafez, A. A, Physico-chemical and sensory properties of cakes supplemented with different concentration of marjoram, Australian Journal of Basic and Applied Sciences, 2012, 6(13), 463-470
Tripathy, B, Satyanarayana, S, Khan, K. A, Raja, K, Mohanty, C, Phytochemical screening and antifungal activity of ethanol and petroleum-ether leaf extracts of Origanum majorana, International Journal of Pharmaceutical Research and Health Sciences, 2016, 4(4), 1320-1323
Örçen, N, In vitro organogenesis and regeneration of Alfalfa (Medicago sativa L.) Domestic Cultivar cv. Kayseri. Fresenius Environmental Bulletin, 2013, 22(9a), 2770-2774
Bayraktar, M, Hayta, S, Parlak, S, Gurel, A, Micropropagation of centennial tertiary relict trees of Liquidambar orientalis Miller through meristematic nodules produced by cultures of primordial shoots, Trees, 2015, 29(4), 999-1009
Krishna, H, Alizadeh, M, Singh, D, Singh, U, Chauhan, N, Eftekhari, M, Sadh, R. K, Somaclonal variations and their applications in horticultural crops improvement, 3 Biotech, 2016, 6(54), 2-18
Singh, S.R, Dalal, S, Singh, R, Dhawan, A.K, Kalia, R.K, Evaluation of genetic fidelity of in vitro raised plants of Dendrocalamus asper (Schult. & Schult. F.) Backer ex K. Heyne using DNA-based markers, Acta Physiologiae Plantarum, 2013, 35, 419-430
Cheruvathur, M.K, Thomas, D. T, Shoot organogenesis from root-drived callus of Rhinacanthus nasutus (L) Kurz. and assessment of clonal fidelity of micropropagated plants using RAPD analysis, Applied Biochemistry and Biotechnology, 2014, 172, 1172-1182
Filiz, E, Koç, İ, Bitki biyoteknolojisinde moleküler markörler, Journal of Agriculture Faculty of Gaziosmanpaşa, 2011, 28(2), 207-214
Dam, A, Paul, S, Bhattacharya, C, Bandyopadhyay, T. K, Effects of culture conditions on multiple shoot induction from inflorescence and RAPD analysis of cloned plants in Limonium sinensis (Girard) Kuntze, var. Golden Diamond, Journal of Plant Biochemis