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Journals / Celal Bayar Üniversitesi Fen Bilimleri Dergisi / 2026 / Cilt: 22 Sayı: 3

Impact of Extraction Solvent on Phenolic Diversity, Protein Content, and Antioxidant Efficiency of Carthamus tinctorius L.

Hafize Dilek Tepe(Manisa Celal Bayar Üniversitesi)0000-0002-6035-6901
Muammer Tepe(MANISA CELAL BAYAR UNIVERSITY, AKHİSAR VOCATIONAL SCHOOL, DEPARTMENT OF CHEMISTRY AND CHEMICAL PROCESS TECHNOLOGIES)0000-0002-1071-0060
Pages
465–474
Publication Date
2026-09-30
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Employing a combination of solvents and antioxidant assays is essential for comprehensive evaluation of plant-based bioactive compounds and their functional potential in food and pharmaceutical applications.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study explored the antioxidant capacity, protein content, and phenolic profile of Carthamus tinctorius L. (safflower) using ethanol (ETOH) and water (AQUA) as extraction solvents.
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Key Findings & Data:The ethanol extract exhibited significantly higher ABTS (1126 μM TE/gDW) and FRAP (1128.69 μM TE/gDW) activities, alongside greater protein content (58.55 µg/gDW), suggesting that ethanol is more effective in extracting lipophilic antioxidants and proteins.
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Conclusion & Impact:Employing a combination of solvents and antioxidant assays is essential for comprehensive evaluation of plant-based bioactive compounds and their functional potential in food and pharmaceutical applications.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Carthamus tinctorius L. · safflower · antioxidant activity

Abstract

This study explored the antioxidant capacity, protein content, and phenolic profile of Carthamus tinctorius L. (safflower) using ethanol (ETOH) and water (AQUA) as extraction solvents. The antioxidant activity was evaluated using ABTS, DPPH, and FRAP assays. The ethanol extract exhibited significantly higher ABTS (1126 μM TE/gDW) and FRAP (1128.69 μM TE/gDW) activities, alongside greater protein content (58.55 µg/gDW), suggesting that ethanol is more effective in extracting lipophilic antioxidants and proteins. Conversely, the aqueous extract demonstrated markedly higher DPPH activity (2500 μM TE/gDW), indicative of abundant hydrophilic compounds with strong radical scavenging properties. The water extract was particularly rich in chlorogenic acid (79.65 µg/g), hesperidin (52.46 µg/g), hyperoside (31.38 µg/g), quercetin, and unique compounds such as ellagic acid and pinoresinol, detected exclusively in this fraction. Employing a combination of solvents and antioxidant assays is essential for comprehensive evaluation of plant-based bioactive compounds and their functional potential in food and pharmaceutical applications.

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Cite This Article

Tepe, H. D., Tepe, M. (2026). Impact of Extraction Solvent on Phenolic Diversity, Protein Content, and Antioxidant Efficiency of Carthamus tinctorius L.. Celal Bayar Üniversitesi Fen Bilimleri Dergisi, Cilt: 22 Sayı: 3, 465–474. https://doi.org/10.18466/cbayarfbe.1826749

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References (32)

  1. . Arslan, D, Özcan, MM. 2011. Evaluation of antioxidant activities of various solvent extracts from Carthamus tinctorius L. (safflower) petals. Food and Bioproducts Processing; 89(2): 170-174.
  2. . Karimi, E, Oskoueian, E, et al. 2012. Phenolic compounds characterization and biological activities of Carthamus tinctorius L. leaves. Molecules; 17(5): 4981-4991.
  3. . Ding, M, Lei, C, et al. 2016. Advances in the chemical constituents of Carthamus tinctorius L. Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology; 18(3): 503-509.
  4. . Liu, CT, Lin, YT, et al. 2014. Total phenolic and flavonoid content and antioxidant activity of extracts from the stems and leaves of Carthamus tinctorius L. Journal of Food and Drug Analysis; 22(3): 386-393.
  5. . Anwar, F, Latif, S, et al. 2007. Moringa oleifera: A food plant with multiple medicinal uses. Phytotherapy Research; 21(1): 17-25. https://doi.org/10.1002/ptr.2023
  6. . Zhang, X, Xie, Y, et al. 2010. Chemical constituents and bioactivities of Carthamus tinctorius L. International Journal of Molecular Sciences; 11(2): 670-683.
  7. . Gao, S, Yu, Y, et al. 2012. Antioxidant and hepatoprotective effects of safflower injection on D-galactosamine-induced liver injury in rats. Journal of Ethnopharmacology; 139(2): 558-564.
  8. . Ghosh, A, Chandra, G, et al. 2014. Bioactive compounds and pharmacological properties of Carthamus tinctorius L.: A review. International Journal of Pharmacy and Pharmaceutical Sciences; 6(4): 9-13.
  9. . Jadhav, HR, Pawar, D. 2018. Pharmacognostic and phytochemical studies of Carthamus tinctorius L. Journal of Pharmacognosy and Phytochemistry; 7(5): 2607-2611.
  10. . Morikawa, T, Xu, F, et al. 2010. Bioactive constituents from Chinese natural medicines. XXI. Triterpene glycosides and acetylenic compounds from the flowers of Carthamus tinctorius L. Chemical and Pharmaceutical Bulletin; 58(7): 882-887.
  11. . Rashed, KN, Butnariu, M. 2014. Isolation and identification of flavonoid compound from safflower (Carthamus tinctorius L.) flowers. Journal of Bioequivalence & Bioavailability; 6(3): 105-108.
  12. . Zhang, H, Wang, Y, et al. 2009. Chemical constituents and pharmacological activities of Carthamus tinctorius L. Journal of Chinese Medicinal Materials; 32(8): 1235-1238.
  13. . Riaz, N, Khan, RA, et al. 2017. Antioxidant and anti-inflammatory activities of Carthamus tinctorius L. extracts. BMC Complementary and Alternative Medicine; 17(1): 10-15.
  14. . Zang, Y, Zhang, X, et al. 2011. Flavonoid glycosides from the flowers of Carthamus tinctorius L. Fitoterapia; 82(4): 568-572.
  15. . Wang, X, Zhang, W, et al. 2015. Protective effect of safflower extract on myocardial ischemia-reperfusion injury in rats. Journal of Ethnopharmacology; 165: 173-180.
  16. . Zhang, Y, Zhang, H, et al. 2007. Anti-inflammatory activity of flavonoids from safflower. Natural Product Research and Development; 19(4): 507-510.
  17. . Li, Y, Xu, Q, et al. 2012. Antioxidant activities of safflower pigments and their protective effect on DNA. Food Chemistry; 135(3): 1865-1870.
  18. . Brand-Williams, W, Cuvelier, ME, et al. 1995. Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology; 28(1): 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
  19. . Velioglu, YS, Mazza, G, et al. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of Agricultural and Food Chemistry; 46(10): 4113-4117. https://doi.org/10.1021/jf9801973
  20. . Benzie, IFF, Strain, JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry; 239(1): 70-76. https://doi.org/10.1006/abio.1996.0292
  21. . Arnao, MB, Cano, A, et al. 2001. The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chemistry; 73(2): 239-244. https://doi.org/10.1016/S0308-8146(00)00324-1
  22. . Warburg, O, Christian, W. 1941. Isolierung und Kristallisation des Gärungsferments Enolase. Biochemische Zeitschrift; 310: 384-421.
  23. . Gören, AC, Çıkrıkçı, S, et al. 2009. Rapid quantitation of curcumin in turmeric via NMR and LC-tandem mass spectrometry. Food Chemistry; 113(4): 1239-1242. https://doi.org/10.1016/j.foodchem.2008.08.014
  24. . Re, R, Pellegrini, N, et al. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine; 26(9-10): 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  25. . Prior, RL, Wu, X, et al. 2005. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry; 53(10): 4290-4302. https://doi.org/10.1021/jf0502698
  26. . Siddhuraju, P, Becker, K. 2003. Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. Journal of Agricultural and Food Chemistry; 51(8): 2144-2155. https://doi.org/10.1021/jf020444+
  27. . Alara, OR, Abdurahman, NH, et al. 2021. Extraction of phenolic compounds: A review. Current Research in Food Science; 4: 200-214. https://doi.org/10.1016/j.crfs.2021.03.011
  28. . Do, QD, Angkawijaya, AE, et al. 2014. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis; 22(3): 296-302. https://doi.org/10.1016/j.jfda.2013.11.001
  29. . Dai, J, Mumper, RJ. 2010. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules; 15(10): 7313-7352. https://doi.org/10.3390/molecules15107313
  30. . Chew, KK, Khoo, MZ, et al. 2011. Effect of ethanol concentration, extraction time and extraction temperature on the recovery of phenolic compounds and antioxidant capacity of Orthosiphon stamineus extracts. International Food Research Journal; 18(4): 1427-1435.
  31. . Rice-Evans, CA, Miller, NJ, et al. 1997. Antioxidant properties of phenolic compounds. Trends in Plant Science; 2(4): 152-159. https://doi.org/10.1016/S1360-1385(97)01018-2
  32. . Apak, R, Gorinstein, S, et al. 2013. Methods of measurement and evaluation of natural antioxidant capacity/activity (IUPAC Technical Report). Pure and Applied Chemistry; 85(5): 957-998. https://doi.org/10.1351/PAC-REP-12-07-15