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Journals / Anestezi Dergisi / 2023 / Cilt: 31 - Sayı: 1

Investigation of Apoptotic Effect of Propofol, Dexmedetomidine and Medetomidine on Oocyte Cumulus Granulosa Cells in Rats

Propofol, Deksmedetomidin ve Medetomidinin Sıçanlarda Oosit Kümülüs Granulosa Hücreleri Üzerine Apoptotik Etkilerinin Araştırılması

Mehmet Sühha BOSTANCI
Gürkan DEMİR
Ayça Taş TUNA
Özcan BUDAK
Havva KOÇYİĞİT
Berrin GÜNAYDIN
Hüseyin ÇAKIROĞLU
Pages
50–54
Publication Date
2023-11-27
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The mean caspase-3 H-score was lower in Group D than that of Group P and M (p

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: We aimed to evaluate the potential apoptotic effects of propofol, dexmedetomidine and medetomidine that were used during oocyte retrieval on cumulus cells in rat ovulation induction model.
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Key Findings & Data:Oocytes collection was performed 10 minutes after the administration of study drugs.
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Conclusion & Impact:The mean caspase-3 H-score was lower in Group D than that of Group P and M (p
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: We aimed to evaluate the potential apoptotic effects of propofol, dexmedetomidine and medetomidine that were used during oocyte retrieval on cumulus cells in rat ovulation induction model. Methods: After the ovulation induction model was created, rats were received dexmedetomidine in Group D, propofol in Group P, medetomidine in Group M. Oocytes collection was performed 10 minutes after the administration of study drugs. For Caspase-3 immunohistochemical evaluation, the staining level was scored in five randomly selected areas and the areas with the highest score were determined. Immunohistochemical staining scoring for each section was performed using a scoring algorithm called H-score. Results: Caspase-3 expression in cumulus cells was found to be lowest in Group D and highest in Group P. The mean caspase-3 H-score was lower in Group D than that of Group P and M (p

Özet

Amaç: Oosit toplama işlemi sırasında kullanılan propofol, deks- medetomidin ve medetomidinin kümülüs hücreleri üzerindeki po- tansiyel apoptotik etkilerini rat ovulasyon indüksiyon modelinde değerlendirmeyi amaçladık. Yöntem: Ovulasyon indüksiyon modeli oluşturulduktan sonra, sı- çanlara Grup D’de deksmedetomidin, Grup P’de propofol, Grup M’de medetomidin verildi. Çalışma ilaçlarının uygulanmasından 10 dakika sonra oosit toplama işlemi yapıldı. Kaspaz-3 immunohis- tokimyasal değerlendirmede rastgele seçilen beş alanda boyanma düzeyi skorlandı ve en yüksek skora sahip alanlar belirlendi. Her bölüm için immünohistokimyasal boyama puanlaması, H-skoru adı verilen bir puanlama algoritması kullanılarak yapıldı. Bulgular: Kümülüs hücrelerinde kaspaz-3 ekspresyonu Grup D’de en düşük, Grup P’de en yüksek bulundu. Ortalama kaspaz-3 H-sko- ru Grup D’de Grup P ve M’ye göre daha düşüktü (p

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Cite This Article

BOSTANCI, M. S., DEMİR, G., TUNA, A. T., BUDAK, Ö., KOÇYİĞİT, H., GÜNAYDIN, B., ÇAKIROĞLU, H. (2023). Investigation of Apoptotic Effect of Propofol, Dexmedetomidine and Medetomidine on Oocyte Cumulus Granulosa Cells in Rats. Anestezi Dergisi, Cilt: 31 - Sayı: 1, 50–54. https://doi.org/10.54875/jarss.2023.88942

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