1984A/G adrenomedullin (rs3814700) gene polymorphism: can it be responsible for unexplained recurrent early pregnancy loss?

Abstract

Objective: The etiology of recurrent miscarriage (RM) is heterogeneous and the current data cannot be able to cover all the aspects of RM. Adrenomedulline (ADM) has been very popular with the discovery of vital functions in maintaining an uneventful pregnancy. Idiopathic RM may result due to defective placentation and implantation process associated with altered ADM function and levels. So, we hypothesized that increased ADM gene polymorphism could play a role in idiopathic RM casesMethods: This prospective case-control study consisted of 60 women; 30 of whom had three consecutive pregnancy losses, who admitted to the outpatient clinic of department of obstetrics and gynecology or department of genetic at our tertiary center. Genomic DNA was extracted from peripheral blood and the frequency of genotypes and alleles of –1984A>G ADM (rs3814700) gene polymorphism was examined by polymerase chain reaction and restriction fragment length polymorphism (PCR/RFLP) methodResults: The mean ages were 29.36±6.22 and 32.15±5.43 in RM and control group, respectively (p=0.314). For -1984A>G polymorphism, the frequency of A allele was 91.7% and 93.3% in RM and control group, respectively (p=0.72). The frequency of G allele was 8.3% and 6.7% in RM and control group, respectively (p=0.72). Regarding the incidence of genotype; AA genotype was 83.3% and 86.7% in RM and control group, respectively (p=0.71). AG genotype was 16.7% and 13.3% in RM and control group, respectively (p=0.71).Conclusions: -1984A>G ADM gene polymorphism does not seem to be associated with idiopathic RM