 The effect of indole-3-acetic acid on some metabolic enzymes in kidney of the second cross maternal mice and their offsprings

Abstract

Objective: Indole-3-acetic acid (IAA) generates reactiveoxygen species (ROS) and excited oxygenintermediates. IAA causes renal dysfunction,hypoglycemia, and myotonia. The first aim of the studyis to investigate the possible indirect effect of the plantgrowth hormone IAA on some renal enzyme activitiessuch as hexokinase (HK), glucose-6-phosphatedehydrogenase (G6PD), 6-phosphogluconatedehydrogenase (6PGD), lactate dehydrogenase (LDH),and malate dehydrogenase (MDH) of second crossoffsprings of the second cross maternal mice (Musmusculus) that are given IAA. The second aim was toinvestigate the possible direct effect of IAA on the samerenal enzymes in second cross maternal mice.Method: Female mice was divided into two groups: IAAadministered group and ethanol control group. Twochemicals used were applied intraperitoneally. IAA wasadministrated to maternal mice as a 1/40 dilution of300 mg/kg body weights in 3-day intervals. Ethanol wasadministrated in the controls. Spectrophotometricmethods were used to determine the activities ofenzymes in the kidney of the second cross-maternalmice and their offsprings.Results: In the second cross-maternal mice, theactivities of LDH, MDH, and G6PD were higher in IAAadministrated animals than in controls. 6PGD and HKshowed decreases in IAA study group compared tocontrol group. In the offsprings of the second crossmaternal mice, the activities of G6PD, HK, and MDHenzymes were decreased, and 6PGD and LDH enzymeswere increased in IAA group compared to controlgroup. Even so differences were not statisticallysignificant.Conclusion: The results obtained suggested thatmetabolic enzymes studied were not affected from IAAtoxicity. This may be due to low dose of IAA.Key words: IAA, metabolic enzymes, mus musculus,kidney.