Dergiler / Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi / 2023 / Cilt: 43 Sayı: 3
Comparative acute toxicity study of Syringodium isoetifolium on aquatic and rodent experimental animals
- Sayfa
- 221–231
- DOI
- —
Özet
This study was designed to explore toxic potential associated with hydroalcoholic extract of Syringodium isoetifolium seagrass (HAESI) on brine shrimp, zebrafish and Wistar albino rats according to the OECD guidelines. The different concentrations of extract were applied to brine shrimp (0.01-1 mg/ml), zebrafish (12.5, 25, 50, 100, 200, 400 mg/ml) and Wistar albino rat (500, 1000, 2000, 2500 and 5000 mg/kg). The control group received distilled water and the studies were carried out as per the OECD guidelines [203 and 423]. The experimental subjects were observed individually for the first 24 hours, with special attention given during the first four hours, thereafter for a prescribed duration. The results of brine shrimp exhibited increased mortality with the increasing concentrations of the extract. Maximum mortality occurred at 1000 Microgram per milliliter (μg/ml) and least mortality happened at 1 μg/ml concentration. The toxic effect on brine shrimp due to the poor elimination of cytotoxic substances from the body at high concentrations and elimination freely occur at low concentrations. Whereas no mortality and behavioural change were observed in the zebrafish and Wistar albino rats irrespective of the concentration.
Abstract
This study was designed to explore toxic potential associated with hydroalcoholic extract of Syringodium isoetifolium seagrass (HAESI) on brine shrimp, zebrafish and Wistar albino rats according to the OECD guidelines. The different concentrations of extract were applied to brine shrimp (0.01-1 mg/ml), zebrafish (12.5, 25, 50, 100, 200, 400 mg/ml) and Wistar albino rat (500, 1000, 2000, 2500 and 5000 mg/kg). The control group received distilled water and the studies were carried out as per the OECD guidelines [203 and 423]. The experimental subjects were observed individually for the first 24 hours, with special attention given during the first four hours, thereafter for a prescribed duration. The results of brine shrimp exhibited increased mortality with the increasing concentrations of the extract. Maximum mortality occurred at 1000 Microgram per milliliter (μg/ml) and least mortality happened at 1 μg/ml concentration. The toxic effect on brine shrimp due to the poor elimination of cytotoxic substances from the body at high concentrations and elimination freely occur at low concentrations. Whereas no mortality and behavioural change were observed in the zebrafish and Wistar albino rats irrespective of the concentration.